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Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model

Since Marine sponge Dysidea avara is regarded as a source of anti-inflammatory compounds, we decided to evaluate its potential anti-psoriatic activity in a psoriasis Imiquimod-induced in the mouse model. Psoriatic mice were treated with three different methanolic extracts of Dysidea avara compared w...

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Autores principales: Khaledi, Mostafa, Sharif Makhmal Zadeh, Behzad, Rezaie, Annahita, Nazemi, Melika, Safdarian, Mehdi, Nabavi, Mohammad Bagher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7703918/
https://www.ncbi.nlm.nih.gov/pubmed/33253155
http://dx.doi.org/10.1371/journal.pone.0241582
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author Khaledi, Mostafa
Sharif Makhmal Zadeh, Behzad
Rezaie, Annahita
Nazemi, Melika
Safdarian, Mehdi
Nabavi, Mohammad Bagher
author_facet Khaledi, Mostafa
Sharif Makhmal Zadeh, Behzad
Rezaie, Annahita
Nazemi, Melika
Safdarian, Mehdi
Nabavi, Mohammad Bagher
author_sort Khaledi, Mostafa
collection PubMed
description Since Marine sponge Dysidea avara is regarded as a source of anti-inflammatory compounds, we decided to evaluate its potential anti-psoriatic activity in a psoriasis Imiquimod-induced in the mouse model. Psoriatic mice were treated with three different methanolic extracts of Dysidea avara compared with betamethasone-treated mice in in- vivo studies. Clinical skin severity was assessed with the psoriasis area index (PASI), whilst ELISA detected the expression of TNF-α, IL-17A, and IL-22. Dysidea avara activity was studied by employing GC-MS (to distinguish compounds), HPTLC (for skin permeation and accumulation), and SEA DOCK to predict single compound potential anti-inflammatory activity. After 7 days of treatment, mice treated with Dysidea avara displayed a dose-dependent, statistically significant improvement compared to controls (p< 0.001). In line with the clinical results, ELISA revealed a statistically significant decrease in IL-22, IL-17A, and TNF-α after treatment; the same SEA DOCK analysis suggests a possible anti-psoriatic activity of the extracts.
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spelling pubmed-77039182020-12-03 Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model Khaledi, Mostafa Sharif Makhmal Zadeh, Behzad Rezaie, Annahita Nazemi, Melika Safdarian, Mehdi Nabavi, Mohammad Bagher PLoS One Research Article Since Marine sponge Dysidea avara is regarded as a source of anti-inflammatory compounds, we decided to evaluate its potential anti-psoriatic activity in a psoriasis Imiquimod-induced in the mouse model. Psoriatic mice were treated with three different methanolic extracts of Dysidea avara compared with betamethasone-treated mice in in- vivo studies. Clinical skin severity was assessed with the psoriasis area index (PASI), whilst ELISA detected the expression of TNF-α, IL-17A, and IL-22. Dysidea avara activity was studied by employing GC-MS (to distinguish compounds), HPTLC (for skin permeation and accumulation), and SEA DOCK to predict single compound potential anti-inflammatory activity. After 7 days of treatment, mice treated with Dysidea avara displayed a dose-dependent, statistically significant improvement compared to controls (p< 0.001). In line with the clinical results, ELISA revealed a statistically significant decrease in IL-22, IL-17A, and TNF-α after treatment; the same SEA DOCK analysis suggests a possible anti-psoriatic activity of the extracts. Public Library of Science 2020-11-30 /pmc/articles/PMC7703918/ /pubmed/33253155 http://dx.doi.org/10.1371/journal.pone.0241582 Text en © 2020 Khaledi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Khaledi, Mostafa
Sharif Makhmal Zadeh, Behzad
Rezaie, Annahita
Nazemi, Melika
Safdarian, Mehdi
Nabavi, Mohammad Bagher
Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model
title Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model
title_full Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model
title_fullStr Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model
title_full_unstemmed Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model
title_short Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model
title_sort chemical profiling and anti-psoriatic activity of marine sponge (dysidea avara) in induced imiquimod-psoriasis-skin model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7703918/
https://www.ncbi.nlm.nih.gov/pubmed/33253155
http://dx.doi.org/10.1371/journal.pone.0241582
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