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Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model
Since Marine sponge Dysidea avara is regarded as a source of anti-inflammatory compounds, we decided to evaluate its potential anti-psoriatic activity in a psoriasis Imiquimod-induced in the mouse model. Psoriatic mice were treated with three different methanolic extracts of Dysidea avara compared w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7703918/ https://www.ncbi.nlm.nih.gov/pubmed/33253155 http://dx.doi.org/10.1371/journal.pone.0241582 |
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author | Khaledi, Mostafa Sharif Makhmal Zadeh, Behzad Rezaie, Annahita Nazemi, Melika Safdarian, Mehdi Nabavi, Mohammad Bagher |
author_facet | Khaledi, Mostafa Sharif Makhmal Zadeh, Behzad Rezaie, Annahita Nazemi, Melika Safdarian, Mehdi Nabavi, Mohammad Bagher |
author_sort | Khaledi, Mostafa |
collection | PubMed |
description | Since Marine sponge Dysidea avara is regarded as a source of anti-inflammatory compounds, we decided to evaluate its potential anti-psoriatic activity in a psoriasis Imiquimod-induced in the mouse model. Psoriatic mice were treated with three different methanolic extracts of Dysidea avara compared with betamethasone-treated mice in in- vivo studies. Clinical skin severity was assessed with the psoriasis area index (PASI), whilst ELISA detected the expression of TNF-α, IL-17A, and IL-22. Dysidea avara activity was studied by employing GC-MS (to distinguish compounds), HPTLC (for skin permeation and accumulation), and SEA DOCK to predict single compound potential anti-inflammatory activity. After 7 days of treatment, mice treated with Dysidea avara displayed a dose-dependent, statistically significant improvement compared to controls (p< 0.001). In line with the clinical results, ELISA revealed a statistically significant decrease in IL-22, IL-17A, and TNF-α after treatment; the same SEA DOCK analysis suggests a possible anti-psoriatic activity of the extracts. |
format | Online Article Text |
id | pubmed-7703918 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-77039182020-12-03 Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model Khaledi, Mostafa Sharif Makhmal Zadeh, Behzad Rezaie, Annahita Nazemi, Melika Safdarian, Mehdi Nabavi, Mohammad Bagher PLoS One Research Article Since Marine sponge Dysidea avara is regarded as a source of anti-inflammatory compounds, we decided to evaluate its potential anti-psoriatic activity in a psoriasis Imiquimod-induced in the mouse model. Psoriatic mice were treated with three different methanolic extracts of Dysidea avara compared with betamethasone-treated mice in in- vivo studies. Clinical skin severity was assessed with the psoriasis area index (PASI), whilst ELISA detected the expression of TNF-α, IL-17A, and IL-22. Dysidea avara activity was studied by employing GC-MS (to distinguish compounds), HPTLC (for skin permeation and accumulation), and SEA DOCK to predict single compound potential anti-inflammatory activity. After 7 days of treatment, mice treated with Dysidea avara displayed a dose-dependent, statistically significant improvement compared to controls (p< 0.001). In line with the clinical results, ELISA revealed a statistically significant decrease in IL-22, IL-17A, and TNF-α after treatment; the same SEA DOCK analysis suggests a possible anti-psoriatic activity of the extracts. Public Library of Science 2020-11-30 /pmc/articles/PMC7703918/ /pubmed/33253155 http://dx.doi.org/10.1371/journal.pone.0241582 Text en © 2020 Khaledi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Khaledi, Mostafa Sharif Makhmal Zadeh, Behzad Rezaie, Annahita Nazemi, Melika Safdarian, Mehdi Nabavi, Mohammad Bagher Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model |
title | Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model |
title_full | Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model |
title_fullStr | Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model |
title_full_unstemmed | Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model |
title_short | Chemical profiling and anti-psoriatic activity of marine sponge (Dysidea avara) in induced imiquimod-psoriasis-skin model |
title_sort | chemical profiling and anti-psoriatic activity of marine sponge (dysidea avara) in induced imiquimod-psoriasis-skin model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7703918/ https://www.ncbi.nlm.nih.gov/pubmed/33253155 http://dx.doi.org/10.1371/journal.pone.0241582 |
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