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Mild heat stress changes the microbiota diversity in the respiratory tract and the cecum of layer-type pullets
The present study aimed to research the effects of cyclic heat environment on the microbial diversity and structure of respiratory tract and cecum of chicken. A total of 360 layer-type pullets at 11 wk of age were subjected to different temperature treatments for 10 wk: constant 22°C; cyclic tempera...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7704960/ https://www.ncbi.nlm.nih.gov/pubmed/33248618 http://dx.doi.org/10.1016/j.psj.2020.09.024 |
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author | Wang, Minghui Lin, Xiaoyan Jiao, Hongchao Uyanga, Victoria Zhao, Jingpeng Wang, Xiaojuan Li, Haifang Zhou, Yunlei Sun, Shuhong Lin, Hai |
author_facet | Wang, Minghui Lin, Xiaoyan Jiao, Hongchao Uyanga, Victoria Zhao, Jingpeng Wang, Xiaojuan Li, Haifang Zhou, Yunlei Sun, Shuhong Lin, Hai |
author_sort | Wang, Minghui |
collection | PubMed |
description | The present study aimed to research the effects of cyclic heat environment on the microbial diversity and structure of respiratory tract and cecum of chicken. A total of 360 layer-type pullets at 11 wk of age were subjected to different temperature treatments for 10 wk: constant 22°C; cyclic temperature 22°C to 24°C, 22°C to 26°C, 22°C to 28°C, 22°C to 30°C; the ambient temperature increased from 10:00, reached the set point within 1 h, and maintained until 18:00, thereafter the temperature was restored to 22°C; and the relative humidity was maintained at 60%. The result showed that feed intake of the chickens on ambient temperature 30°C group was significantly lower than that of the chickens on ambient temperature 24°C. The white blood cell, red blood cell, lymphocyte, hemoglobin, and pecked-cell volume content were highest at 24°C on 14, 16, and 18 wk. The ratio of CD3(+)CD4(+)/CD3(+)CD8(+) T cells was lowest at 30°C. Meanwhile, the abundance of cecum bacteria in chickens at 30°C was lower than that at 24°C. Cyclic heat environment temperature treatment did not significantly affect the concentration of secretory immunoglobulin A in chicken bronchoalveolar lavage fluid (BALF) levels during 10 wk of trial. The diversity index analysis showed that the effect of 24°C on the cecum flora of chickens was optimal. Abundance of Firmicutes bacteria in the lung flora and cecum flora was lower at 30°C than at 24°C group. Similarly, the microorganism, Brevibacillus in the BALF was also significantly lower at 24°C. In conclusion, cyclic 24°C treatment was beneficial for the feed intake, blood routine indexes, microflora structure of the cecum, and respiratory tract in laying pullets. |
format | Online Article Text |
id | pubmed-7704960 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77049602020-12-08 Mild heat stress changes the microbiota diversity in the respiratory tract and the cecum of layer-type pullets Wang, Minghui Lin, Xiaoyan Jiao, Hongchao Uyanga, Victoria Zhao, Jingpeng Wang, Xiaojuan Li, Haifang Zhou, Yunlei Sun, Shuhong Lin, Hai Poult Sci Microbiology and Food Safety The present study aimed to research the effects of cyclic heat environment on the microbial diversity and structure of respiratory tract and cecum of chicken. A total of 360 layer-type pullets at 11 wk of age were subjected to different temperature treatments for 10 wk: constant 22°C; cyclic temperature 22°C to 24°C, 22°C to 26°C, 22°C to 28°C, 22°C to 30°C; the ambient temperature increased from 10:00, reached the set point within 1 h, and maintained until 18:00, thereafter the temperature was restored to 22°C; and the relative humidity was maintained at 60%. The result showed that feed intake of the chickens on ambient temperature 30°C group was significantly lower than that of the chickens on ambient temperature 24°C. The white blood cell, red blood cell, lymphocyte, hemoglobin, and pecked-cell volume content were highest at 24°C on 14, 16, and 18 wk. The ratio of CD3(+)CD4(+)/CD3(+)CD8(+) T cells was lowest at 30°C. Meanwhile, the abundance of cecum bacteria in chickens at 30°C was lower than that at 24°C. Cyclic heat environment temperature treatment did not significantly affect the concentration of secretory immunoglobulin A in chicken bronchoalveolar lavage fluid (BALF) levels during 10 wk of trial. The diversity index analysis showed that the effect of 24°C on the cecum flora of chickens was optimal. Abundance of Firmicutes bacteria in the lung flora and cecum flora was lower at 30°C than at 24°C group. Similarly, the microorganism, Brevibacillus in the BALF was also significantly lower at 24°C. In conclusion, cyclic 24°C treatment was beneficial for the feed intake, blood routine indexes, microflora structure of the cecum, and respiratory tract in laying pullets. Elsevier 2020-09-18 /pmc/articles/PMC7704960/ /pubmed/33248618 http://dx.doi.org/10.1016/j.psj.2020.09.024 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Microbiology and Food Safety Wang, Minghui Lin, Xiaoyan Jiao, Hongchao Uyanga, Victoria Zhao, Jingpeng Wang, Xiaojuan Li, Haifang Zhou, Yunlei Sun, Shuhong Lin, Hai Mild heat stress changes the microbiota diversity in the respiratory tract and the cecum of layer-type pullets |
title | Mild heat stress changes the microbiota diversity in the respiratory tract and the cecum of layer-type pullets |
title_full | Mild heat stress changes the microbiota diversity in the respiratory tract and the cecum of layer-type pullets |
title_fullStr | Mild heat stress changes the microbiota diversity in the respiratory tract and the cecum of layer-type pullets |
title_full_unstemmed | Mild heat stress changes the microbiota diversity in the respiratory tract and the cecum of layer-type pullets |
title_short | Mild heat stress changes the microbiota diversity in the respiratory tract and the cecum of layer-type pullets |
title_sort | mild heat stress changes the microbiota diversity in the respiratory tract and the cecum of layer-type pullets |
topic | Microbiology and Food Safety |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7704960/ https://www.ncbi.nlm.nih.gov/pubmed/33248618 http://dx.doi.org/10.1016/j.psj.2020.09.024 |
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