Bioinformatics analysis of long non-coding RNAs involved in nerve regeneration following sciatic nerve injury

Little is known about the role of epigenetic modification in axon regeneration following peripheral nerve injury. The purpose of the present study was to investigate the role of long non-coding RNAs (lncRNAs) in the regulation of axon regeneration. We used bioinformatics to perform microarray analys...

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Autores principales: Jia, Yuanyuan, Zhang, Ming, Li, Pei, Tang, Wenbo, Liu, Yao, Hu, Yiwen, Cui, Qingjun, Liu, Ming, Jiang, Jingjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705388/
https://www.ncbi.nlm.nih.gov/pubmed/33241745
http://dx.doi.org/10.1177/1744806920971918
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author Jia, Yuanyuan
Zhang, Ming
Li, Pei
Tang, Wenbo
Liu, Yao
Hu, Yiwen
Cui, Qingjun
Liu, Ming
Jiang, Jingjing
author_facet Jia, Yuanyuan
Zhang, Ming
Li, Pei
Tang, Wenbo
Liu, Yao
Hu, Yiwen
Cui, Qingjun
Liu, Ming
Jiang, Jingjing
author_sort Jia, Yuanyuan
collection PubMed
description Little is known about the role of epigenetic modification in axon regeneration following peripheral nerve injury. The purpose of the present study was to investigate the role of long non-coding RNAs (lncRNAs) in the regulation of axon regeneration. We used bioinformatics to perform microarray analysis and screened total 476 lncRNAs and 129 microRNAs (miRNAs) of differentially expressed genes after sciatic nerve injury in mice. lncRNA-GM4208 and lncRNA-GM30085 were examined, and the changes in lncRNA expression in the L4–L6 dorsal root ganglia (DRG) following sciatic nerve crush injury were analyzed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression of lncRNAs in the DRG changed, indicating that they might be related to nerve regeneration in the DRG following peripheral nerve injury.
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spelling pubmed-77053882020-12-07 Bioinformatics analysis of long non-coding RNAs involved in nerve regeneration following sciatic nerve injury Jia, Yuanyuan Zhang, Ming Li, Pei Tang, Wenbo Liu, Yao Hu, Yiwen Cui, Qingjun Liu, Ming Jiang, Jingjing Mol Pain Research Article Little is known about the role of epigenetic modification in axon regeneration following peripheral nerve injury. The purpose of the present study was to investigate the role of long non-coding RNAs (lncRNAs) in the regulation of axon regeneration. We used bioinformatics to perform microarray analysis and screened total 476 lncRNAs and 129 microRNAs (miRNAs) of differentially expressed genes after sciatic nerve injury in mice. lncRNA-GM4208 and lncRNA-GM30085 were examined, and the changes in lncRNA expression in the L4–L6 dorsal root ganglia (DRG) following sciatic nerve crush injury were analyzed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression of lncRNAs in the DRG changed, indicating that they might be related to nerve regeneration in the DRG following peripheral nerve injury. SAGE Publications 2020-11-26 /pmc/articles/PMC7705388/ /pubmed/33241745 http://dx.doi.org/10.1177/1744806920971918 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Article
Jia, Yuanyuan
Zhang, Ming
Li, Pei
Tang, Wenbo
Liu, Yao
Hu, Yiwen
Cui, Qingjun
Liu, Ming
Jiang, Jingjing
Bioinformatics analysis of long non-coding RNAs involved in nerve regeneration following sciatic nerve injury
title Bioinformatics analysis of long non-coding RNAs involved in nerve regeneration following sciatic nerve injury
title_full Bioinformatics analysis of long non-coding RNAs involved in nerve regeneration following sciatic nerve injury
title_fullStr Bioinformatics analysis of long non-coding RNAs involved in nerve regeneration following sciatic nerve injury
title_full_unstemmed Bioinformatics analysis of long non-coding RNAs involved in nerve regeneration following sciatic nerve injury
title_short Bioinformatics analysis of long non-coding RNAs involved in nerve regeneration following sciatic nerve injury
title_sort bioinformatics analysis of long non-coding rnas involved in nerve regeneration following sciatic nerve injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705388/
https://www.ncbi.nlm.nih.gov/pubmed/33241745
http://dx.doi.org/10.1177/1744806920971918
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