ELISA detection of SARS-CoV-2 antibodies in saliva

To facilitate containment of the COVID-19 pandemic currently active in the United States and across the world, options for easy, non-invasive antibody testing are required. Here we have adapted a commercially available, serum-based enzyme-linked immunosorbent assay (ELISA) for use with saliva sample...

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Autores principales: MacMullan, Melanie A., Ibrayeva, Albina, Trettner, Kylie, Deming, Laura, Das, Sudipta, Tran, Frances, Moreno, Jose Ricardo, Casian, Joseph G., Chellamuthu, Prithivi, Kraft, Jeffrey, Kozak, Kenneth, Turner, Fred E., Slepnev, Vladimir I., Le Page, Lydia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705674/
https://www.ncbi.nlm.nih.gov/pubmed/33257702
http://dx.doi.org/10.1038/s41598-020-77555-4
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author MacMullan, Melanie A.
Ibrayeva, Albina
Trettner, Kylie
Deming, Laura
Das, Sudipta
Tran, Frances
Moreno, Jose Ricardo
Casian, Joseph G.
Chellamuthu, Prithivi
Kraft, Jeffrey
Kozak, Kenneth
Turner, Fred E.
Slepnev, Vladimir I.
Le Page, Lydia M.
author_facet MacMullan, Melanie A.
Ibrayeva, Albina
Trettner, Kylie
Deming, Laura
Das, Sudipta
Tran, Frances
Moreno, Jose Ricardo
Casian, Joseph G.
Chellamuthu, Prithivi
Kraft, Jeffrey
Kozak, Kenneth
Turner, Fred E.
Slepnev, Vladimir I.
Le Page, Lydia M.
author_sort MacMullan, Melanie A.
collection PubMed
description To facilitate containment of the COVID-19 pandemic currently active in the United States and across the world, options for easy, non-invasive antibody testing are required. Here we have adapted a commercially available, serum-based enzyme-linked immunosorbent assay (ELISA) for use with saliva samples, achieving 84.2% sensitivity and 100% specificity in a set of 149 clinical samples. This strategy will enable widespread, affordable testing for patients who experienced this disease, whilst minimizing exposure risk for healthcare workers.
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spelling pubmed-77056742020-12-02 ELISA detection of SARS-CoV-2 antibodies in saliva MacMullan, Melanie A. Ibrayeva, Albina Trettner, Kylie Deming, Laura Das, Sudipta Tran, Frances Moreno, Jose Ricardo Casian, Joseph G. Chellamuthu, Prithivi Kraft, Jeffrey Kozak, Kenneth Turner, Fred E. Slepnev, Vladimir I. Le Page, Lydia M. Sci Rep Article To facilitate containment of the COVID-19 pandemic currently active in the United States and across the world, options for easy, non-invasive antibody testing are required. Here we have adapted a commercially available, serum-based enzyme-linked immunosorbent assay (ELISA) for use with saliva samples, achieving 84.2% sensitivity and 100% specificity in a set of 149 clinical samples. This strategy will enable widespread, affordable testing for patients who experienced this disease, whilst minimizing exposure risk for healthcare workers. Nature Publishing Group UK 2020-11-30 /pmc/articles/PMC7705674/ /pubmed/33257702 http://dx.doi.org/10.1038/s41598-020-77555-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
MacMullan, Melanie A.
Ibrayeva, Albina
Trettner, Kylie
Deming, Laura
Das, Sudipta
Tran, Frances
Moreno, Jose Ricardo
Casian, Joseph G.
Chellamuthu, Prithivi
Kraft, Jeffrey
Kozak, Kenneth
Turner, Fred E.
Slepnev, Vladimir I.
Le Page, Lydia M.
ELISA detection of SARS-CoV-2 antibodies in saliva
title ELISA detection of SARS-CoV-2 antibodies in saliva
title_full ELISA detection of SARS-CoV-2 antibodies in saliva
title_fullStr ELISA detection of SARS-CoV-2 antibodies in saliva
title_full_unstemmed ELISA detection of SARS-CoV-2 antibodies in saliva
title_short ELISA detection of SARS-CoV-2 antibodies in saliva
title_sort elisa detection of sars-cov-2 antibodies in saliva
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705674/
https://www.ncbi.nlm.nih.gov/pubmed/33257702
http://dx.doi.org/10.1038/s41598-020-77555-4
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