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Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets

Cell and tissue engineering approaches for articular cartilage regeneration increasingly focus on mesenchymal stem cells (MSCs) as allogeneic cell sources, based on availability and innate chondrogenic potential. Many MSCs exhibit chondrogenic potential as three-dimensional (3D) cultures (i.e. pelle...

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Autores principales: Thorp, Hallie, Kim, Kyungsook, Kondo, Makoto, Grainger, David W., Okano, Teruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705723/
https://www.ncbi.nlm.nih.gov/pubmed/33257787
http://dx.doi.org/10.1038/s41598-020-77842-0
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author Thorp, Hallie
Kim, Kyungsook
Kondo, Makoto
Grainger, David W.
Okano, Teruo
author_facet Thorp, Hallie
Kim, Kyungsook
Kondo, Makoto
Grainger, David W.
Okano, Teruo
author_sort Thorp, Hallie
collection PubMed
description Cell and tissue engineering approaches for articular cartilage regeneration increasingly focus on mesenchymal stem cells (MSCs) as allogeneic cell sources, based on availability and innate chondrogenic potential. Many MSCs exhibit chondrogenic potential as three-dimensional (3D) cultures (i.e. pellets and seeded biomaterial scaffolds) in vitro; however, these constructs present engraftment, biocompatibility, and cell functionality limitations in vivo. Cell sheet technology maintains cell functionality as scaffold-free constructs while enabling direct cell transplantation from in vitro culture to targeted sites in vivo. The present study aims to develop transplantable hyaline-like cartilage constructs by stimulating MSC chondrogenic differentiation as cell sheets. To achieve this goal, 3D MSC sheets are prepared, exploiting spontaneous post-detachment cell sheet contraction, and chondrogenically induced. Results support 3D MSC sheets’ chondrogenic differentiation to hyaline cartilage in vitro via post-contraction cytoskeletal reorganization and structural transformations. These 3D cell sheets’ initial thickness and cellular densities may also modulate MSC-derived chondrocyte hypertrophy in vitro. Furthermore, chondrogenically differentiated cell sheets adhere directly to cartilage surfaces via retention of adhesion molecules while maintaining the cell sheets’ characteristics. Together, these data support the utility of cell sheet technology for fabricating scaffold-free, hyaline-like cartilage constructs from MSCs for future transplantable articular cartilage regeneration therapies.
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spelling pubmed-77057232020-12-02 Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets Thorp, Hallie Kim, Kyungsook Kondo, Makoto Grainger, David W. Okano, Teruo Sci Rep Article Cell and tissue engineering approaches for articular cartilage regeneration increasingly focus on mesenchymal stem cells (MSCs) as allogeneic cell sources, based on availability and innate chondrogenic potential. Many MSCs exhibit chondrogenic potential as three-dimensional (3D) cultures (i.e. pellets and seeded biomaterial scaffolds) in vitro; however, these constructs present engraftment, biocompatibility, and cell functionality limitations in vivo. Cell sheet technology maintains cell functionality as scaffold-free constructs while enabling direct cell transplantation from in vitro culture to targeted sites in vivo. The present study aims to develop transplantable hyaline-like cartilage constructs by stimulating MSC chondrogenic differentiation as cell sheets. To achieve this goal, 3D MSC sheets are prepared, exploiting spontaneous post-detachment cell sheet contraction, and chondrogenically induced. Results support 3D MSC sheets’ chondrogenic differentiation to hyaline cartilage in vitro via post-contraction cytoskeletal reorganization and structural transformations. These 3D cell sheets’ initial thickness and cellular densities may also modulate MSC-derived chondrocyte hypertrophy in vitro. Furthermore, chondrogenically differentiated cell sheets adhere directly to cartilage surfaces via retention of adhesion molecules while maintaining the cell sheets’ characteristics. Together, these data support the utility of cell sheet technology for fabricating scaffold-free, hyaline-like cartilage constructs from MSCs for future transplantable articular cartilage regeneration therapies. Nature Publishing Group UK 2020-11-30 /pmc/articles/PMC7705723/ /pubmed/33257787 http://dx.doi.org/10.1038/s41598-020-77842-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Thorp, Hallie
Kim, Kyungsook
Kondo, Makoto
Grainger, David W.
Okano, Teruo
Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets
title Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets
title_full Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets
title_fullStr Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets
title_full_unstemmed Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets
title_short Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets
title_sort fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705723/
https://www.ncbi.nlm.nih.gov/pubmed/33257787
http://dx.doi.org/10.1038/s41598-020-77842-0
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