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The local concentration of Ca(2+) correlates with BMP7 expression and osseointegration in patients with total hip arthroplasty
BACKGROUND: A successful osseointegration of total hip arthroplasty (THA) relies on the interplay of implant surface and bone marrow microenvironment. This study was undertaken to investigate the impact of perioperative biochemical molecules (Ca(2+), Mg(2+), Zn(2+), VD, PTH) on the bone marrow osteo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706042/ https://www.ncbi.nlm.nih.gov/pubmed/33256843 http://dx.doi.org/10.1186/s13018-020-02090-x |
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author | Fu, Xiaodong Wang, Weili Li, Xiaomiao Gao, Yingjian Li, Hao Shen, Yi |
author_facet | Fu, Xiaodong Wang, Weili Li, Xiaomiao Gao, Yingjian Li, Hao Shen, Yi |
author_sort | Fu, Xiaodong |
collection | PubMed |
description | BACKGROUND: A successful osseointegration of total hip arthroplasty (THA) relies on the interplay of implant surface and bone marrow microenvironment. This study was undertaken to investigate the impact of perioperative biochemical molecules (Ca(2+), Mg(2+), Zn(2+), VD, PTH) on the bone marrow osteogenetic factors (BMP2, BMP7, Stro-1(+) cells) in the metaphyseal region of the femoral head, and further on the bone mineral density (BMD) of Gruen R3. METHODS: Bone marrow aspirates were obtained from the discarded metaphysis region of the femoral head in 51 patients with THA. Flow cytometry was used to measure the Stro-1(+) expressing cells. ELISA was used to measure the concentrations of bone morphologic proteins (BMP2 and BMP7) and the content of TRACP5b in serum. TRAP staining was used to detect the osteoclast activity in the hip joint. The perioperative concentrations of the biochemical molecules above were measured by radioimmunoassay. The BMD of Gruen zone R3 was examined at 6 months after THA, using dual-energy X-ray absorptiometry (DEXA). RESULTS: Our data demonstrated that the concentration of Ca(2+) was positively correlated with BMP7 expression, and with the postoperative BMD of Gruen zone R3. However, the concentration of Mg(2+) had little impact on the R3 BMD, although it was negatively correlated with the expression of BMP7. Osteoclast activity in hip joint tissue of patients with femoral neck fractures was increased. Compared with the patients before THA, the levels of TRACP5b in serum of patients after THA were decreased. The data also suggested that the other biochemical molecules, such as Zn(2+), VD, and PTH, were not significantly correlated with any bone marrow osteogenetic factors (BMP2, BMP7, Stro-1(+) cells). The postoperative R3 BMD of patients of different gender and age had no significant difference. CONCLUSIONS: These results indicate the local concentration of Ca(2+) may be an indicator for the prognosis of THA patients. |
format | Online Article Text |
id | pubmed-7706042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-77060422020-12-01 The local concentration of Ca(2+) correlates with BMP7 expression and osseointegration in patients with total hip arthroplasty Fu, Xiaodong Wang, Weili Li, Xiaomiao Gao, Yingjian Li, Hao Shen, Yi J Orthop Surg Res Research Article BACKGROUND: A successful osseointegration of total hip arthroplasty (THA) relies on the interplay of implant surface and bone marrow microenvironment. This study was undertaken to investigate the impact of perioperative biochemical molecules (Ca(2+), Mg(2+), Zn(2+), VD, PTH) on the bone marrow osteogenetic factors (BMP2, BMP7, Stro-1(+) cells) in the metaphyseal region of the femoral head, and further on the bone mineral density (BMD) of Gruen R3. METHODS: Bone marrow aspirates were obtained from the discarded metaphysis region of the femoral head in 51 patients with THA. Flow cytometry was used to measure the Stro-1(+) expressing cells. ELISA was used to measure the concentrations of bone morphologic proteins (BMP2 and BMP7) and the content of TRACP5b in serum. TRAP staining was used to detect the osteoclast activity in the hip joint. The perioperative concentrations of the biochemical molecules above were measured by radioimmunoassay. The BMD of Gruen zone R3 was examined at 6 months after THA, using dual-energy X-ray absorptiometry (DEXA). RESULTS: Our data demonstrated that the concentration of Ca(2+) was positively correlated with BMP7 expression, and with the postoperative BMD of Gruen zone R3. However, the concentration of Mg(2+) had little impact on the R3 BMD, although it was negatively correlated with the expression of BMP7. Osteoclast activity in hip joint tissue of patients with femoral neck fractures was increased. Compared with the patients before THA, the levels of TRACP5b in serum of patients after THA were decreased. The data also suggested that the other biochemical molecules, such as Zn(2+), VD, and PTH, were not significantly correlated with any bone marrow osteogenetic factors (BMP2, BMP7, Stro-1(+) cells). The postoperative R3 BMD of patients of different gender and age had no significant difference. CONCLUSIONS: These results indicate the local concentration of Ca(2+) may be an indicator for the prognosis of THA patients. BioMed Central 2020-11-30 /pmc/articles/PMC7706042/ /pubmed/33256843 http://dx.doi.org/10.1186/s13018-020-02090-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Fu, Xiaodong Wang, Weili Li, Xiaomiao Gao, Yingjian Li, Hao Shen, Yi The local concentration of Ca(2+) correlates with BMP7 expression and osseointegration in patients with total hip arthroplasty |
title | The local concentration of Ca(2+) correlates with BMP7 expression and osseointegration in patients with total hip arthroplasty |
title_full | The local concentration of Ca(2+) correlates with BMP7 expression and osseointegration in patients with total hip arthroplasty |
title_fullStr | The local concentration of Ca(2+) correlates with BMP7 expression and osseointegration in patients with total hip arthroplasty |
title_full_unstemmed | The local concentration of Ca(2+) correlates with BMP7 expression and osseointegration in patients with total hip arthroplasty |
title_short | The local concentration of Ca(2+) correlates with BMP7 expression and osseointegration in patients with total hip arthroplasty |
title_sort | local concentration of ca(2+) correlates with bmp7 expression and osseointegration in patients with total hip arthroplasty |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706042/ https://www.ncbi.nlm.nih.gov/pubmed/33256843 http://dx.doi.org/10.1186/s13018-020-02090-x |
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