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Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS
Lung transplantation is a potentially life-saving therapy for patients with terminal respiratory illnesses. Long-term survival is limited by the development of a variety of opportunistic infections and rejection. Optimal means of differential diagnosis of infection and rejection have not been establ...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706120/ https://www.ncbi.nlm.nih.gov/pubmed/33313416 http://dx.doi.org/10.1515/med-2020-0232 |
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author | Wang, Qing Feng, Jing Zhang, Ji Shi, Lingzhi Jin, Zhixian Liu, Dong Wu, Bo Chen, Jingyu |
author_facet | Wang, Qing Feng, Jing Zhang, Ji Shi, Lingzhi Jin, Zhixian Liu, Dong Wu, Bo Chen, Jingyu |
author_sort | Wang, Qing |
collection | PubMed |
description | Lung transplantation is a potentially life-saving therapy for patients with terminal respiratory illnesses. Long-term survival is limited by the development of a variety of opportunistic infections and rejection. Optimal means of differential diagnosis of infection and rejection have not been established. With these challenges in mind, we tried to use transbronchial lung biopsy (TBLB) rapid on-site cytological evaluation (ROSE), metagenomic next-generation sequencing (mNGS), and routine histologic examination to timely distinguish infection and rejection, and accurately detect etiologic pathogens. We reviewed the medical records of all patients diagnosed with infection or rejection by these means from December 2017 to September 2018 in our center. We identified seven recipients whose clinical course was complicated by infection or rejection. Three patients were diagnosed with acute rejection, organizing pneumonia, and acute fibrinoid organizing pneumonia, respectively. Four of the seven patients were diagnosed with infections, including Pneumocystis carinii pneumonia, cytomegalovirus, Aspergillus, and bacterial pneumonia. These patients recovered after proper treatment. TBLB + ROSE + mNGS might be a good method to accurately detect etiologic pathogens, which may help us to facilitate the use of targeted and precision medicine therapy in postoperative complications and avoid unnecessary potential adverse effects of drugs. |
format | Online Article Text |
id | pubmed-7706120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-77061202020-12-10 Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS Wang, Qing Feng, Jing Zhang, Ji Shi, Lingzhi Jin, Zhixian Liu, Dong Wu, Bo Chen, Jingyu Open Med (Wars) Research Article Lung transplantation is a potentially life-saving therapy for patients with terminal respiratory illnesses. Long-term survival is limited by the development of a variety of opportunistic infections and rejection. Optimal means of differential diagnosis of infection and rejection have not been established. With these challenges in mind, we tried to use transbronchial lung biopsy (TBLB) rapid on-site cytological evaluation (ROSE), metagenomic next-generation sequencing (mNGS), and routine histologic examination to timely distinguish infection and rejection, and accurately detect etiologic pathogens. We reviewed the medical records of all patients diagnosed with infection or rejection by these means from December 2017 to September 2018 in our center. We identified seven recipients whose clinical course was complicated by infection or rejection. Three patients were diagnosed with acute rejection, organizing pneumonia, and acute fibrinoid organizing pneumonia, respectively. Four of the seven patients were diagnosed with infections, including Pneumocystis carinii pneumonia, cytomegalovirus, Aspergillus, and bacterial pneumonia. These patients recovered after proper treatment. TBLB + ROSE + mNGS might be a good method to accurately detect etiologic pathogens, which may help us to facilitate the use of targeted and precision medicine therapy in postoperative complications and avoid unnecessary potential adverse effects of drugs. De Gruyter 2020-10-01 /pmc/articles/PMC7706120/ /pubmed/33313416 http://dx.doi.org/10.1515/med-2020-0232 Text en © 2020 Qing Wang et al., published by De Gruyter http://creativecommons.org/licenses/by/4.0 This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Article Wang, Qing Feng, Jing Zhang, Ji Shi, Lingzhi Jin, Zhixian Liu, Dong Wu, Bo Chen, Jingyu Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS |
title | Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS |
title_full | Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS |
title_fullStr | Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS |
title_full_unstemmed | Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS |
title_short | Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS |
title_sort | diagnosis of complication in lung transplantation by tblb + rose + mngs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706120/ https://www.ncbi.nlm.nih.gov/pubmed/33313416 http://dx.doi.org/10.1515/med-2020-0232 |
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