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microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9
Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are critical processes that are involved in atherosclerosis. The aim of this study was to explore the role of microRNA-491-5p (miR-491-5p) in the progression of atherosclerosis by regulating the growth and migration of VSMC...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706122/ https://www.ncbi.nlm.nih.gov/pubmed/33313408 http://dx.doi.org/10.1515/med-2020-0047 |
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author | He, Zhonghan Wang, Yayun He, Qin Chen, Manhua |
author_facet | He, Zhonghan Wang, Yayun He, Qin Chen, Manhua |
author_sort | He, Zhonghan |
collection | PubMed |
description | Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are critical processes that are involved in atherosclerosis. The aim of this study was to explore the role of microRNA-491-5p (miR-491-5p) in the progression of atherosclerosis by regulating the growth and migration of VSMCs. In this study, we showed that the expression of miR-491-5p was downregulated in the atherosclerotic plaque tissues and plasma samples of the patients with atherosclerosis. The bioinformatic analysis and dual-luciferase reporter assay identified that matrix metallopeptidase-9 (MMP-9) was a target gene of miR-491-5p. The results showed a significant upregulation of MMP-9 in the atherosclerotic plaque tissues and plasma samples. Subsequently, the results also showed that downregulation of miR-491-5p significantly promoted the proliferation and migration of VSMCs and inhibited the apoptosis in VSMCs. Furthermore, we detected the effects of miR-491-5p mimic on the growth and migration of VSMCs, and the results illustrated that miR-491-5p mimic could inhibit the proliferation and migration of VSMCs and promote the apoptosis of VSMCs. Notably, MMP-9 plasmid could reverse all the effects of miR-491-5p mimic on VSMCs. Collectively, our study provides the first evidence that miR-491-5p inhibited the growth and migration of VSMCs by targeting MMP-9, which might provide new biomarkers and potential therapeutic targets for atherosclerosis treatment. |
format | Online Article Text |
id | pubmed-7706122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-77061222020-12-10 microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9 He, Zhonghan Wang, Yayun He, Qin Chen, Manhua Open Med (Wars) Research Article Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are critical processes that are involved in atherosclerosis. The aim of this study was to explore the role of microRNA-491-5p (miR-491-5p) in the progression of atherosclerosis by regulating the growth and migration of VSMCs. In this study, we showed that the expression of miR-491-5p was downregulated in the atherosclerotic plaque tissues and plasma samples of the patients with atherosclerosis. The bioinformatic analysis and dual-luciferase reporter assay identified that matrix metallopeptidase-9 (MMP-9) was a target gene of miR-491-5p. The results showed a significant upregulation of MMP-9 in the atherosclerotic plaque tissues and plasma samples. Subsequently, the results also showed that downregulation of miR-491-5p significantly promoted the proliferation and migration of VSMCs and inhibited the apoptosis in VSMCs. Furthermore, we detected the effects of miR-491-5p mimic on the growth and migration of VSMCs, and the results illustrated that miR-491-5p mimic could inhibit the proliferation and migration of VSMCs and promote the apoptosis of VSMCs. Notably, MMP-9 plasmid could reverse all the effects of miR-491-5p mimic on VSMCs. Collectively, our study provides the first evidence that miR-491-5p inhibited the growth and migration of VSMCs by targeting MMP-9, which might provide new biomarkers and potential therapeutic targets for atherosclerosis treatment. De Gruyter 2020-06-02 /pmc/articles/PMC7706122/ /pubmed/33313408 http://dx.doi.org/10.1515/med-2020-0047 Text en © 2020 Zhonghan He et al., published by De Gruyter http://creativecommons.org/licenses/by/4.0 This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Article He, Zhonghan Wang, Yayun He, Qin Chen, Manhua microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9 |
title | microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9 |
title_full | microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9 |
title_fullStr | microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9 |
title_full_unstemmed | microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9 |
title_short | microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9 |
title_sort | microrna-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706122/ https://www.ncbi.nlm.nih.gov/pubmed/33313408 http://dx.doi.org/10.1515/med-2020-0047 |
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