Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity

BACKGROUND: One ongoing concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-target activity is challenging. Here, we present SMRT-OTS and Nano-OTS, two novel, amplification-free, long-re...

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Detalles Bibliográficos
Autores principales: Höijer, Ida, Johansson, Josefin, Gudmundsson, Sanna, Chin, Chen-Shan, Bunikis, Ignas, Häggqvist, Susana, Emmanouilidou, Anastasia, Wilbe, Maria, den Hoed, Marcel, Bondeson, Marie-Louise, Feuk, Lars, Gyllensten, Ulf, Ameur, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706270/
https://www.ncbi.nlm.nih.gov/pubmed/33261648
http://dx.doi.org/10.1186/s13059-020-02206-w

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