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Sequencing and analysis of the complete mitochondrial genome of Crocidura tanakae from China and its phylogenetic analysis
The complete mitogenome sequence of Crocidura tanakae was determined using long PCR. The genome was 16,969 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heav...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706458/ https://www.ncbi.nlm.nih.gov/pubmed/33365730 http://dx.doi.org/10.1080/23802359.2019.1659118 |
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author | Zhi-Min, Jin Ke-Song, Qin Bo-Qi, Li Jun-Sheng, Zhang He, Cai Jin-Xun, Li Zhu, Wang Zhu, Liu |
author_facet | Zhi-Min, Jin Ke-Song, Qin Bo-Qi, Li Jun-Sheng, Zhang He, Cai Jin-Xun, Li Zhu, Wang Zhu, Liu |
author_sort | Zhi-Min, Jin |
collection | PubMed |
description | The complete mitogenome sequence of Crocidura tanakae was determined using long PCR. The genome was 16,969 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (32.5%), C (22.3%), T (31.9%), and G (13.3%). The base compositions present clearly the A–T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. The five Crocidura species formed a monophyletic group with the high bootstrap value (100%) in all examinations. This study verifies the evolutionary status of C. tanakae in Soricidae at the molecular level. The mitochondrial genome would be a significant supplement for the C. tanakae genetic background. |
format | Online Article Text |
id | pubmed-7706458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-77064582020-12-22 Sequencing and analysis of the complete mitochondrial genome of Crocidura tanakae from China and its phylogenetic analysis Zhi-Min, Jin Ke-Song, Qin Bo-Qi, Li Jun-Sheng, Zhang He, Cai Jin-Xun, Li Zhu, Wang Zhu, Liu Mitochondrial DNA B Resour Mitogenome Announcement The complete mitogenome sequence of Crocidura tanakae was determined using long PCR. The genome was 16,969 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (32.5%), C (22.3%), T (31.9%), and G (13.3%). The base compositions present clearly the A–T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. The five Crocidura species formed a monophyletic group with the high bootstrap value (100%) in all examinations. This study verifies the evolutionary status of C. tanakae in Soricidae at the molecular level. The mitochondrial genome would be a significant supplement for the C. tanakae genetic background. Taylor & Francis 2019-08-29 /pmc/articles/PMC7706458/ /pubmed/33365730 http://dx.doi.org/10.1080/23802359.2019.1659118 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Mitogenome Announcement Zhi-Min, Jin Ke-Song, Qin Bo-Qi, Li Jun-Sheng, Zhang He, Cai Jin-Xun, Li Zhu, Wang Zhu, Liu Sequencing and analysis of the complete mitochondrial genome of Crocidura tanakae from China and its phylogenetic analysis |
title | Sequencing and analysis of the complete mitochondrial genome of Crocidura tanakae from China and its phylogenetic analysis |
title_full | Sequencing and analysis of the complete mitochondrial genome of Crocidura tanakae from China and its phylogenetic analysis |
title_fullStr | Sequencing and analysis of the complete mitochondrial genome of Crocidura tanakae from China and its phylogenetic analysis |
title_full_unstemmed | Sequencing and analysis of the complete mitochondrial genome of Crocidura tanakae from China and its phylogenetic analysis |
title_short | Sequencing and analysis of the complete mitochondrial genome of Crocidura tanakae from China and its phylogenetic analysis |
title_sort | sequencing and analysis of the complete mitochondrial genome of crocidura tanakae from china and its phylogenetic analysis |
topic | Mitogenome Announcement |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706458/ https://www.ncbi.nlm.nih.gov/pubmed/33365730 http://dx.doi.org/10.1080/23802359.2019.1659118 |
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