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Role of Scx(+)/Sox9(+) cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice

A multipotent cell population co-expressing a basic-helix-loop-helix transcription factor scleraxis (Scx) and SRY-box 9 (Sox9) has been shown to contribute to the establishment of entheses (tendon attachment sites) during mouse embryonic development. The present study aimed to investigate the involv...

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Autores principales: Ideo, Katsumasa, Tokunaga, Takuya, Shukunami, Chisa, Takimoto, Aki, Yoshimoto, Yuki, Yonemitsu, Ryuji, Karasugi, Tatsuki, Mizuta, Hiroshi, Hiraki, Yuji, Miyamoto, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707462/
https://www.ncbi.nlm.nih.gov/pubmed/33259516
http://dx.doi.org/10.1371/journal.pone.0242286
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author Ideo, Katsumasa
Tokunaga, Takuya
Shukunami, Chisa
Takimoto, Aki
Yoshimoto, Yuki
Yonemitsu, Ryuji
Karasugi, Tatsuki
Mizuta, Hiroshi
Hiraki, Yuji
Miyamoto, Takeshi
author_facet Ideo, Katsumasa
Tokunaga, Takuya
Shukunami, Chisa
Takimoto, Aki
Yoshimoto, Yuki
Yonemitsu, Ryuji
Karasugi, Tatsuki
Mizuta, Hiroshi
Hiraki, Yuji
Miyamoto, Takeshi
author_sort Ideo, Katsumasa
collection PubMed
description A multipotent cell population co-expressing a basic-helix-loop-helix transcription factor scleraxis (Scx) and SRY-box 9 (Sox9) has been shown to contribute to the establishment of entheses (tendon attachment sites) during mouse embryonic development. The present study aimed to investigate the involvement of Scx(+)/Sox9(+) cells in the postnatal formation of fibrocartilaginous entheses and in the healing process after injury, using ScxGFP transgenic mice. We demonstrate that Scx(+)/Sox9(+) cells are localized in layers at the insertion site during the postnatal formation of fibrocartilaginous entheses of supraspinatus tendon until postnatal 3 weeks. Further, these cells were rarely seen at postnatal 6 weeks, when mature fibrocartilaginous entheses were formed. Furthermore, we investigated the involvement of Scx(+)/Sox9(+) cells in the healing process after supraspinatus tendon enthesis injury, comparing the responses of 20- and 3-week-old mice. In the healing process of 20-week-old mice with disorganized fibrovascular tissue in response to injury, a small number of Scx(+)/Sox9(+) cells transiently appeared from 1 week after injury, but they were rarely seen at 4 weeks after injury. Meanwhile, in 3-week-old mice, a thin layer of fibrocartilaginous tissue with calcification was formed at healing enthesis at 4 weeks after injury. From 1 to 2 weeks after injury, more Scx(+)/Sox9(+) cells, widely distributed at the injured site, were seen compared with the 20-week-old mice. At 4 weeks after injury, these cells were located near the surface of the recreated fibrocartilaginous layer. This spatiotemporal localization pattern of Scx(+)/Sox9(+) cells at the injured enthesis in our 3-week-old mouse model was similar to that in postnatal fibrocartilaginous enthesis formation. These findings indicate that Scx(+)/Sox9(+) cells may have a role as entheseal progenitor-like cells during postnatal maturation of fibrocartilaginous entheses and healing after injury in a manner similar to that seen in embryonic development.
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spelling pubmed-77074622020-12-08 Role of Scx(+)/Sox9(+) cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice Ideo, Katsumasa Tokunaga, Takuya Shukunami, Chisa Takimoto, Aki Yoshimoto, Yuki Yonemitsu, Ryuji Karasugi, Tatsuki Mizuta, Hiroshi Hiraki, Yuji Miyamoto, Takeshi PLoS One Research Article A multipotent cell population co-expressing a basic-helix-loop-helix transcription factor scleraxis (Scx) and SRY-box 9 (Sox9) has been shown to contribute to the establishment of entheses (tendon attachment sites) during mouse embryonic development. The present study aimed to investigate the involvement of Scx(+)/Sox9(+) cells in the postnatal formation of fibrocartilaginous entheses and in the healing process after injury, using ScxGFP transgenic mice. We demonstrate that Scx(+)/Sox9(+) cells are localized in layers at the insertion site during the postnatal formation of fibrocartilaginous entheses of supraspinatus tendon until postnatal 3 weeks. Further, these cells were rarely seen at postnatal 6 weeks, when mature fibrocartilaginous entheses were formed. Furthermore, we investigated the involvement of Scx(+)/Sox9(+) cells in the healing process after supraspinatus tendon enthesis injury, comparing the responses of 20- and 3-week-old mice. In the healing process of 20-week-old mice with disorganized fibrovascular tissue in response to injury, a small number of Scx(+)/Sox9(+) cells transiently appeared from 1 week after injury, but they were rarely seen at 4 weeks after injury. Meanwhile, in 3-week-old mice, a thin layer of fibrocartilaginous tissue with calcification was formed at healing enthesis at 4 weeks after injury. From 1 to 2 weeks after injury, more Scx(+)/Sox9(+) cells, widely distributed at the injured site, were seen compared with the 20-week-old mice. At 4 weeks after injury, these cells were located near the surface of the recreated fibrocartilaginous layer. This spatiotemporal localization pattern of Scx(+)/Sox9(+) cells at the injured enthesis in our 3-week-old mouse model was similar to that in postnatal fibrocartilaginous enthesis formation. These findings indicate that Scx(+)/Sox9(+) cells may have a role as entheseal progenitor-like cells during postnatal maturation of fibrocartilaginous entheses and healing after injury in a manner similar to that seen in embryonic development. Public Library of Science 2020-12-01 /pmc/articles/PMC7707462/ /pubmed/33259516 http://dx.doi.org/10.1371/journal.pone.0242286 Text en © 2020 Ideo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ideo, Katsumasa
Tokunaga, Takuya
Shukunami, Chisa
Takimoto, Aki
Yoshimoto, Yuki
Yonemitsu, Ryuji
Karasugi, Tatsuki
Mizuta, Hiroshi
Hiraki, Yuji
Miyamoto, Takeshi
Role of Scx(+)/Sox9(+) cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice
title Role of Scx(+)/Sox9(+) cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice
title_full Role of Scx(+)/Sox9(+) cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice
title_fullStr Role of Scx(+)/Sox9(+) cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice
title_full_unstemmed Role of Scx(+)/Sox9(+) cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice
title_short Role of Scx(+)/Sox9(+) cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice
title_sort role of scx(+)/sox9(+) cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707462/
https://www.ncbi.nlm.nih.gov/pubmed/33259516
http://dx.doi.org/10.1371/journal.pone.0242286
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