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Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses
Salt stress affects the metabolic homeostasis of medicinal plants. However, medicinal plants are sessile organisms that cannot escape from salt stress. They acclimatize themselves to the stress by reprogramming their metabolic pathways. Lonicerae Japonicae Flos (LJF) with strong antioxidant activity...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707481/ https://www.ncbi.nlm.nih.gov/pubmed/33259548 http://dx.doi.org/10.1371/journal.pone.0243111 |
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author | Cai, Zhichen Chen, Huan Chen, Jiajia Yang, Rong Zou, Lisi Wang, Chengcheng Chen, Jiali Tan, Mengxia Mei, Yuqi Wei, Lifang Yin, Shengxin Liu, Xunhong |
author_facet | Cai, Zhichen Chen, Huan Chen, Jiajia Yang, Rong Zou, Lisi Wang, Chengcheng Chen, Jiali Tan, Mengxia Mei, Yuqi Wei, Lifang Yin, Shengxin Liu, Xunhong |
author_sort | Cai, Zhichen |
collection | PubMed |
description | Salt stress affects the metabolic homeostasis of medicinal plants. However, medicinal plants are sessile organisms that cannot escape from salt stress. They acclimatize themselves to the stress by reprogramming their metabolic pathways. Lonicerae Japonicae Flos (LJF) with strong antioxidant activity is commonly used in traditional Chinese medicine, tea, and beverage. Nevertheless, the variation of integrated metabolites in LJF under different salt stresses remains unclear. In this study, High Performance Liquid Chromatography tandem triple time-of-flight mass spectrometry (HPLC- triple TOF-MS/MS) coupled with multivariate statistical analysis was applied to comparatively investigate the metabolites changes in LJF under different salt stress (0, 100, 200, 300 mM NaCl). Total 47 differential metabolites were screened from 79 metabolites identified in LJF under different salt stress. Low salt-treated group (100 mM NaCl) appeared to be the best group in terms of relative contents (peak areas) of the wide variety in bioactive components. Additionally, the phenylpropanoid pathway, monoterpenoid biosynthesis, glycolysis, TCA cycle, and alkaloid biosynthesis were disturbed in all salt-stress LJF. The results showed that LJF metabolisms were dramatically induced under salt stress and the quality of LJF was better under low salt stress. The study provides novel insights into the quality assessment of LJF under salt stress and a beneficial framework of knowledge applied to improvement the medicinal value of LJF. |
format | Online Article Text |
id | pubmed-7707481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-77074812020-12-08 Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses Cai, Zhichen Chen, Huan Chen, Jiajia Yang, Rong Zou, Lisi Wang, Chengcheng Chen, Jiali Tan, Mengxia Mei, Yuqi Wei, Lifang Yin, Shengxin Liu, Xunhong PLoS One Research Article Salt stress affects the metabolic homeostasis of medicinal plants. However, medicinal plants are sessile organisms that cannot escape from salt stress. They acclimatize themselves to the stress by reprogramming their metabolic pathways. Lonicerae Japonicae Flos (LJF) with strong antioxidant activity is commonly used in traditional Chinese medicine, tea, and beverage. Nevertheless, the variation of integrated metabolites in LJF under different salt stresses remains unclear. In this study, High Performance Liquid Chromatography tandem triple time-of-flight mass spectrometry (HPLC- triple TOF-MS/MS) coupled with multivariate statistical analysis was applied to comparatively investigate the metabolites changes in LJF under different salt stress (0, 100, 200, 300 mM NaCl). Total 47 differential metabolites were screened from 79 metabolites identified in LJF under different salt stress. Low salt-treated group (100 mM NaCl) appeared to be the best group in terms of relative contents (peak areas) of the wide variety in bioactive components. Additionally, the phenylpropanoid pathway, monoterpenoid biosynthesis, glycolysis, TCA cycle, and alkaloid biosynthesis were disturbed in all salt-stress LJF. The results showed that LJF metabolisms were dramatically induced under salt stress and the quality of LJF was better under low salt stress. The study provides novel insights into the quality assessment of LJF under salt stress and a beneficial framework of knowledge applied to improvement the medicinal value of LJF. Public Library of Science 2020-12-01 /pmc/articles/PMC7707481/ /pubmed/33259548 http://dx.doi.org/10.1371/journal.pone.0243111 Text en © 2020 Cai et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Cai, Zhichen Chen, Huan Chen, Jiajia Yang, Rong Zou, Lisi Wang, Chengcheng Chen, Jiali Tan, Mengxia Mei, Yuqi Wei, Lifang Yin, Shengxin Liu, Xunhong Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses |
title | Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses |
title_full | Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses |
title_fullStr | Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses |
title_full_unstemmed | Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses |
title_short | Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses |
title_sort | metabolomics characterizes the metabolic changes of lonicerae japonicae flos under different salt stresses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707481/ https://www.ncbi.nlm.nih.gov/pubmed/33259548 http://dx.doi.org/10.1371/journal.pone.0243111 |
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