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Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses

Salt stress affects the metabolic homeostasis of medicinal plants. However, medicinal plants are sessile organisms that cannot escape from salt stress. They acclimatize themselves to the stress by reprogramming their metabolic pathways. Lonicerae Japonicae Flos (LJF) with strong antioxidant activity...

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Autores principales: Cai, Zhichen, Chen, Huan, Chen, Jiajia, Yang, Rong, Zou, Lisi, Wang, Chengcheng, Chen, Jiali, Tan, Mengxia, Mei, Yuqi, Wei, Lifang, Yin, Shengxin, Liu, Xunhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707481/
https://www.ncbi.nlm.nih.gov/pubmed/33259548
http://dx.doi.org/10.1371/journal.pone.0243111
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author Cai, Zhichen
Chen, Huan
Chen, Jiajia
Yang, Rong
Zou, Lisi
Wang, Chengcheng
Chen, Jiali
Tan, Mengxia
Mei, Yuqi
Wei, Lifang
Yin, Shengxin
Liu, Xunhong
author_facet Cai, Zhichen
Chen, Huan
Chen, Jiajia
Yang, Rong
Zou, Lisi
Wang, Chengcheng
Chen, Jiali
Tan, Mengxia
Mei, Yuqi
Wei, Lifang
Yin, Shengxin
Liu, Xunhong
author_sort Cai, Zhichen
collection PubMed
description Salt stress affects the metabolic homeostasis of medicinal plants. However, medicinal plants are sessile organisms that cannot escape from salt stress. They acclimatize themselves to the stress by reprogramming their metabolic pathways. Lonicerae Japonicae Flos (LJF) with strong antioxidant activity is commonly used in traditional Chinese medicine, tea, and beverage. Nevertheless, the variation of integrated metabolites in LJF under different salt stresses remains unclear. In this study, High Performance Liquid Chromatography tandem triple time-of-flight mass spectrometry (HPLC- triple TOF-MS/MS) coupled with multivariate statistical analysis was applied to comparatively investigate the metabolites changes in LJF under different salt stress (0, 100, 200, 300 mM NaCl). Total 47 differential metabolites were screened from 79 metabolites identified in LJF under different salt stress. Low salt-treated group (100 mM NaCl) appeared to be the best group in terms of relative contents (peak areas) of the wide variety in bioactive components. Additionally, the phenylpropanoid pathway, monoterpenoid biosynthesis, glycolysis, TCA cycle, and alkaloid biosynthesis were disturbed in all salt-stress LJF. The results showed that LJF metabolisms were dramatically induced under salt stress and the quality of LJF was better under low salt stress. The study provides novel insights into the quality assessment of LJF under salt stress and a beneficial framework of knowledge applied to improvement the medicinal value of LJF.
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spelling pubmed-77074812020-12-08 Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses Cai, Zhichen Chen, Huan Chen, Jiajia Yang, Rong Zou, Lisi Wang, Chengcheng Chen, Jiali Tan, Mengxia Mei, Yuqi Wei, Lifang Yin, Shengxin Liu, Xunhong PLoS One Research Article Salt stress affects the metabolic homeostasis of medicinal plants. However, medicinal plants are sessile organisms that cannot escape from salt stress. They acclimatize themselves to the stress by reprogramming their metabolic pathways. Lonicerae Japonicae Flos (LJF) with strong antioxidant activity is commonly used in traditional Chinese medicine, tea, and beverage. Nevertheless, the variation of integrated metabolites in LJF under different salt stresses remains unclear. In this study, High Performance Liquid Chromatography tandem triple time-of-flight mass spectrometry (HPLC- triple TOF-MS/MS) coupled with multivariate statistical analysis was applied to comparatively investigate the metabolites changes in LJF under different salt stress (0, 100, 200, 300 mM NaCl). Total 47 differential metabolites were screened from 79 metabolites identified in LJF under different salt stress. Low salt-treated group (100 mM NaCl) appeared to be the best group in terms of relative contents (peak areas) of the wide variety in bioactive components. Additionally, the phenylpropanoid pathway, monoterpenoid biosynthesis, glycolysis, TCA cycle, and alkaloid biosynthesis were disturbed in all salt-stress LJF. The results showed that LJF metabolisms were dramatically induced under salt stress and the quality of LJF was better under low salt stress. The study provides novel insights into the quality assessment of LJF under salt stress and a beneficial framework of knowledge applied to improvement the medicinal value of LJF. Public Library of Science 2020-12-01 /pmc/articles/PMC7707481/ /pubmed/33259548 http://dx.doi.org/10.1371/journal.pone.0243111 Text en © 2020 Cai et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cai, Zhichen
Chen, Huan
Chen, Jiajia
Yang, Rong
Zou, Lisi
Wang, Chengcheng
Chen, Jiali
Tan, Mengxia
Mei, Yuqi
Wei, Lifang
Yin, Shengxin
Liu, Xunhong
Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses
title Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses
title_full Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses
title_fullStr Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses
title_full_unstemmed Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses
title_short Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses
title_sort metabolomics characterizes the metabolic changes of lonicerae japonicae flos under different salt stresses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707481/
https://www.ncbi.nlm.nih.gov/pubmed/33259548
http://dx.doi.org/10.1371/journal.pone.0243111
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