Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae

A multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in Cornu Saigae Tataricae. Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes...

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Autores principales: Chen, Yufei, Yang, Yaya, Qian, Yinhuan, Gblinwon, Roselyn Tehzee, Jiao, Zhaoqun, Chen, Liqun, Lin, Ling, Zheng, Yang, Yang, Huan, Shen, Yuping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Mito Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707849/
https://www.ncbi.nlm.nih.gov/pubmed/33365929
http://dx.doi.org/10.1080/23802359.2019.1666667
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author Chen, Yufei
Yang, Yaya
Qian, Yinhuan
Gblinwon, Roselyn Tehzee
Jiao, Zhaoqun
Chen, Liqun
Lin, Ling
Zheng, Yang
Yang, Huan
Shen, Yuping
author_facet Chen, Yufei
Yang, Yaya
Qian, Yinhuan
Gblinwon, Roselyn Tehzee
Jiao, Zhaoqun
Chen, Liqun
Lin, Ling
Zheng, Yang
Yang, Huan
Shen, Yuping
author_sort Chen, Yufei
collection PubMed
description A multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in Cornu Saigae Tataricae. Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes. When a mixture of antelope hornand fake species was assayed, it exhibited powerful differentiation capability. 5 out of 12 batches of commercialproducts were identified to be counterfeited or adulterated with Ovis aries Linnaeus and/or Capra hircus Linnaeus. It is readily applicable in routine analysis for identification of sham or adulterants of Cornu Saigae Tataricae.
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spelling pubmed-77078492020-12-22 Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae Chen, Yufei Yang, Yaya Qian, Yinhuan Gblinwon, Roselyn Tehzee Jiao, Zhaoqun Chen, Liqun Lin, Ling Zheng, Yang Yang, Huan Shen, Yuping Mitochondrial DNA B Resour Mito Communication A multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in Cornu Saigae Tataricae. Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes. When a mixture of antelope hornand fake species was assayed, it exhibited powerful differentiation capability. 5 out of 12 batches of commercialproducts were identified to be counterfeited or adulterated with Ovis aries Linnaeus and/or Capra hircus Linnaeus. It is readily applicable in routine analysis for identification of sham or adulterants of Cornu Saigae Tataricae. Taylor & Francis 2019-09-25 /pmc/articles/PMC7707849/ /pubmed/33365929 http://dx.doi.org/10.1080/23802359.2019.1666667 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Mito Communication
Chen, Yufei
Yang, Yaya
Qian, Yinhuan
Gblinwon, Roselyn Tehzee
Jiao, Zhaoqun
Chen, Liqun
Lin, Ling
Zheng, Yang
Yang, Huan
Shen, Yuping
Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae
title Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae
title_full Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae
title_fullStr Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae
title_full_unstemmed Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae
title_short Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae
title_sort harnessing multiplex pcr assay targeting specific mitochondrial dna elements for simultaneous identification of antelope species in cornu saigae tataricae
topic Mito Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707849/
https://www.ncbi.nlm.nih.gov/pubmed/33365929
http://dx.doi.org/10.1080/23802359.2019.1666667
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