Cargando…

Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS

BACKGROUND: We previously identified the urinary biomarkers to diagnose calcium deficiency and nutritional rickets by ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF MS/MS). To find biomarkers of calcium deficiency and further confirm these biom...

Descripción completa

Detalles Bibliográficos
Autores principales: Meng, Fanyu, Fan, Lina, Sun, Lin, Yu, Qingli, Wang, Maoqing, Sun, Changhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708254/
https://www.ncbi.nlm.nih.gov/pubmed/33292300
http://dx.doi.org/10.1186/s12986-020-00507-2
_version_ 1783617526940303360
author Meng, Fanyu
Fan, Lina
Sun, Lin
Yu, Qingli
Wang, Maoqing
Sun, Changhao
author_facet Meng, Fanyu
Fan, Lina
Sun, Lin
Yu, Qingli
Wang, Maoqing
Sun, Changhao
author_sort Meng, Fanyu
collection PubMed
description BACKGROUND: We previously identified the urinary biomarkers to diagnose calcium deficiency and nutritional rickets by ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF MS/MS). To find biomarkers of calcium deficiency and further confirm these biomarkers in serum, we performed serum metabolomics analysis of calcium-deficient rats. METHODS: A calcium-deficient rat model was established with a low-calcium diet for 12 weeks. Serum metabolomics based UPLC/Q-TOF MS/MS and multivariate statistical analysis was performed to identify the alterations in metabolites associated with calcium deficiency in rats. RESULTS: Bone mineral density, serum parathyroid hormone and alkaline phosphatase were significantly decreased in the low-calcium diet group (LCG) compared to the normal calcium diet group (NCG). Serum metabolic-profiling analysis could definitively distinguish between the LCG and NCG and identified 24 calcium-deficient biomarkers. Three metabolites (indoxyl sulfate, phosphate, and taurine) of the 24 biomarkers were found in our previous urinary metabolomics study of rats with a calcium deficiency and nutritional rickets. The areas under the curve (AUCs) of these three biomarkers were greater than 0.8, and the combination of any two biomarkers was higher than 0.95. CONCLUSION: Dietary calcium deficiency induced the alterations of metabolites in the serum of rats, and the three identified biomarkers had relatively high diagnostic values for calcium deficiency in rats.
format Online
Article
Text
id pubmed-7708254
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-77082542020-12-02 Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS Meng, Fanyu Fan, Lina Sun, Lin Yu, Qingli Wang, Maoqing Sun, Changhao Nutr Metab (Lond) Research BACKGROUND: We previously identified the urinary biomarkers to diagnose calcium deficiency and nutritional rickets by ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF MS/MS). To find biomarkers of calcium deficiency and further confirm these biomarkers in serum, we performed serum metabolomics analysis of calcium-deficient rats. METHODS: A calcium-deficient rat model was established with a low-calcium diet for 12 weeks. Serum metabolomics based UPLC/Q-TOF MS/MS and multivariate statistical analysis was performed to identify the alterations in metabolites associated with calcium deficiency in rats. RESULTS: Bone mineral density, serum parathyroid hormone and alkaline phosphatase were significantly decreased in the low-calcium diet group (LCG) compared to the normal calcium diet group (NCG). Serum metabolic-profiling analysis could definitively distinguish between the LCG and NCG and identified 24 calcium-deficient biomarkers. Three metabolites (indoxyl sulfate, phosphate, and taurine) of the 24 biomarkers were found in our previous urinary metabolomics study of rats with a calcium deficiency and nutritional rickets. The areas under the curve (AUCs) of these three biomarkers were greater than 0.8, and the combination of any two biomarkers was higher than 0.95. CONCLUSION: Dietary calcium deficiency induced the alterations of metabolites in the serum of rats, and the three identified biomarkers had relatively high diagnostic values for calcium deficiency in rats. BioMed Central 2020-11-23 /pmc/articles/PMC7708254/ /pubmed/33292300 http://dx.doi.org/10.1186/s12986-020-00507-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Meng, Fanyu
Fan, Lina
Sun, Lin
Yu, Qingli
Wang, Maoqing
Sun, Changhao
Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS
title Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS
title_full Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS
title_fullStr Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS
title_full_unstemmed Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS
title_short Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS
title_sort serum biomarkers of the calcium-deficient rats identified by metabolomics based on uplc/q-tof ms/ms
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708254/
https://www.ncbi.nlm.nih.gov/pubmed/33292300
http://dx.doi.org/10.1186/s12986-020-00507-2
work_keys_str_mv AT mengfanyu serumbiomarkersofthecalciumdeficientratsidentifiedbymetabolomicsbasedonuplcqtofmsms
AT fanlina serumbiomarkersofthecalciumdeficientratsidentifiedbymetabolomicsbasedonuplcqtofmsms
AT sunlin serumbiomarkersofthecalciumdeficientratsidentifiedbymetabolomicsbasedonuplcqtofmsms
AT yuqingli serumbiomarkersofthecalciumdeficientratsidentifiedbymetabolomicsbasedonuplcqtofmsms
AT wangmaoqing serumbiomarkersofthecalciumdeficientratsidentifiedbymetabolomicsbasedonuplcqtofmsms
AT sunchanghao serumbiomarkersofthecalciumdeficientratsidentifiedbymetabolomicsbasedonuplcqtofmsms