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DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencing

DNA aptamers generated by cell-SELEX against bacterial cells have gained increased interest as novel and cost-effective affinity reagents for cell labelling, imaging and biosensing. Here we describe the selection and identification of DNA aptamers for bacterial cells using a combined approach based...

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Detalles Bibliográficos
Autores principales: Kolm, Claudia, Cervenka, Isabella, Aschl, Ulrich J., Baumann, Niklas, Jakwerth, Stefan, Krska, Rudolf, Mach, Robert L., Sommer, Regina, DeRosa, Maria C., Kirschner, Alexander K. T., Farnleitner, Andreas H., Reischer, Georg H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708460/
https://www.ncbi.nlm.nih.gov/pubmed/33262379
http://dx.doi.org/10.1038/s41598-020-77221-9