Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity

Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based a...

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Autores principales: Gorbachev, Dmitry A., Staroverov, Dmitry B., Lukyanov, Konstantin A., Sarkisyan, Karen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709005/
https://www.ncbi.nlm.nih.gov/pubmed/33233801
http://dx.doi.org/10.3390/ijms21228800
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author Gorbachev, Dmitry A.
Staroverov, Dmitry B.
Lukyanov, Konstantin A.
Sarkisyan, Karen S.
author_facet Gorbachev, Dmitry A.
Staroverov, Dmitry B.
Lukyanov, Konstantin A.
Sarkisyan, Karen S.
author_sort Gorbachev, Dmitry A.
collection PubMed
description Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.
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spelling pubmed-77090052020-12-03 Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity Gorbachev, Dmitry A. Staroverov, Dmitry B. Lukyanov, Konstantin A. Sarkisyan, Karen S. Int J Mol Sci Article Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures. MDPI 2020-11-20 /pmc/articles/PMC7709005/ /pubmed/33233801 http://dx.doi.org/10.3390/ijms21228800 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gorbachev, Dmitry A.
Staroverov, Dmitry B.
Lukyanov, Konstantin A.
Sarkisyan, Karen S.
Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity
title Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity
title_full Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity
title_fullStr Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity
title_full_unstemmed Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity
title_short Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity
title_sort genetically encoded red photosensitizers with enhanced phototoxicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709005/
https://www.ncbi.nlm.nih.gov/pubmed/33233801
http://dx.doi.org/10.3390/ijms21228800
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