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Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients

Vitamin B12 has been widely related to methionine metabolism, which is an essential component for biological methylation reactions, including DNA methylation. However, the relationship between vitamin B12 and DNA methylation is still controversial. In addition, there is increasing evidence for the a...

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Autores principales: Boughanem, Hatim, Hernandez-Alonso, Pablo, Tinahones, Alberto, Babio, Nancy, Salas-Salvadó, Jordi, Tinahones, Francisco J., Macias-Gonzalez, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709022/
https://www.ncbi.nlm.nih.gov/pubmed/33233812
http://dx.doi.org/10.3390/nu12113567
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author Boughanem, Hatim
Hernandez-Alonso, Pablo
Tinahones, Alberto
Babio, Nancy
Salas-Salvadó, Jordi
Tinahones, Francisco J.
Macias-Gonzalez, Manuel
author_facet Boughanem, Hatim
Hernandez-Alonso, Pablo
Tinahones, Alberto
Babio, Nancy
Salas-Salvadó, Jordi
Tinahones, Francisco J.
Macias-Gonzalez, Manuel
author_sort Boughanem, Hatim
collection PubMed
description Vitamin B12 has been widely related to methionine metabolism, which is an essential component for biological methylation reactions, including DNA methylation. However, the relationship between vitamin B12 and DNA methylation is still controversial. In addition, there is increasing evidence for the association between vitamin B12 and the risk of colorectal cancer (CRC), although results of this association need to be assessed with caution. For this purpose, we hypothesized that serum vitamin B12 could be associated with global DNA methylation in the CRC context. To test this hypothesis, we studied the association between global DNA methylation through long interspersed nuclear element-1 (LINE1) in CRC patients under the 25th percentile of serum vitamin B12. We found that the high vitamin B12 group had low LINE1 methylation in both tumor area and peripheral blood mononuclear cells (PBMCs) than the low serum vitamin B12 group. LINE1 methylation levels were significantly lower in tumor area compared to the adjacent tumor-free area, only in the high vitamin B12 group. LINE1 methylation in visceral adipose tissue (VAT) and PBMCs were correlated with tumoral, inflammatory, and insulin metabolism markers. However, the interaction between LINE1 methylation and vitamin B12 levels was associated with neoadjuvant therapy in the regression analysis only in men, suggesting a beneficial relationship. In conclusion, our results reported an inverse association between DNA methylation and vitamin B12 in the CRC context, which suggests that vitamin B12 may be implicated in an epigenetic state or mediation in CRC.
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spelling pubmed-77090222020-12-03 Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients Boughanem, Hatim Hernandez-Alonso, Pablo Tinahones, Alberto Babio, Nancy Salas-Salvadó, Jordi Tinahones, Francisco J. Macias-Gonzalez, Manuel Nutrients Article Vitamin B12 has been widely related to methionine metabolism, which is an essential component for biological methylation reactions, including DNA methylation. However, the relationship between vitamin B12 and DNA methylation is still controversial. In addition, there is increasing evidence for the association between vitamin B12 and the risk of colorectal cancer (CRC), although results of this association need to be assessed with caution. For this purpose, we hypothesized that serum vitamin B12 could be associated with global DNA methylation in the CRC context. To test this hypothesis, we studied the association between global DNA methylation through long interspersed nuclear element-1 (LINE1) in CRC patients under the 25th percentile of serum vitamin B12. We found that the high vitamin B12 group had low LINE1 methylation in both tumor area and peripheral blood mononuclear cells (PBMCs) than the low serum vitamin B12 group. LINE1 methylation levels were significantly lower in tumor area compared to the adjacent tumor-free area, only in the high vitamin B12 group. LINE1 methylation in visceral adipose tissue (VAT) and PBMCs were correlated with tumoral, inflammatory, and insulin metabolism markers. However, the interaction between LINE1 methylation and vitamin B12 levels was associated with neoadjuvant therapy in the regression analysis only in men, suggesting a beneficial relationship. In conclusion, our results reported an inverse association between DNA methylation and vitamin B12 in the CRC context, which suggests that vitamin B12 may be implicated in an epigenetic state or mediation in CRC. MDPI 2020-11-20 /pmc/articles/PMC7709022/ /pubmed/33233812 http://dx.doi.org/10.3390/nu12113567 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boughanem, Hatim
Hernandez-Alonso, Pablo
Tinahones, Alberto
Babio, Nancy
Salas-Salvadó, Jordi
Tinahones, Francisco J.
Macias-Gonzalez, Manuel
Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients
title Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients
title_full Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients
title_fullStr Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients
title_full_unstemmed Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients
title_short Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients
title_sort association between serum vitamin b12 and global dna methylation in colorectal cancer patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709022/
https://www.ncbi.nlm.nih.gov/pubmed/33233812
http://dx.doi.org/10.3390/nu12113567
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