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A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells

Lipid catabolism and anabolism changes play a role in stemness acquisition by cancer cells, and cancer stem cells (CSCs) are particularly dependent on the activity of the enzymes involved in these processes. Lipidomic changes could play a role in CSCs’ ability to cause disease relapse and chemoresis...

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Autores principales: Rivas Serna, Irma Magaly, Romito, Ilaria, Maugeri, Andrea, Lo Re, Oriana, Giallongo, Sebastiano, Mazzoccoli, Gianluigi, Oben, Jude A., Li Volti, Giovanni, Mazza, Tommaso, Alisi, Anna, Vinciguerra, Manlio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709039/
https://www.ncbi.nlm.nih.gov/pubmed/33182805
http://dx.doi.org/10.3390/ijms21228452
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author Rivas Serna, Irma Magaly
Romito, Ilaria
Maugeri, Andrea
Lo Re, Oriana
Giallongo, Sebastiano
Mazzoccoli, Gianluigi
Oben, Jude A.
Li Volti, Giovanni
Mazza, Tommaso
Alisi, Anna
Vinciguerra, Manlio
author_facet Rivas Serna, Irma Magaly
Romito, Ilaria
Maugeri, Andrea
Lo Re, Oriana
Giallongo, Sebastiano
Mazzoccoli, Gianluigi
Oben, Jude A.
Li Volti, Giovanni
Mazza, Tommaso
Alisi, Anna
Vinciguerra, Manlio
author_sort Rivas Serna, Irma Magaly
collection PubMed
description Lipid catabolism and anabolism changes play a role in stemness acquisition by cancer cells, and cancer stem cells (CSCs) are particularly dependent on the activity of the enzymes involved in these processes. Lipidomic changes could play a role in CSCs’ ability to cause disease relapse and chemoresistance. The exploration of lipid composition and metabolism changes in CSCs in the context of hepatocellular cancer (HCC) is still incomplete and their lipidomic scenario continues to be elusive. We aimed to evaluate through high-throughput mass spectrometry (MS)-based lipidomics the levels of the members of the six major classes of sphingolipids and phospholipids in two HCC cell lines (HepG2 and Huh-7) silenced for the expression of histone variant macroH2A1 (favoring stemness acquisition), or silenced for the expression of focal adhesion tyrosine kinase (FAK) (hindering aggressiveness and stemness). Transcriptomic changes were evaluated by RNA sequencing as well. We found definite lipidomic and transcriptomic changes in the HCC lines upon knockdown (KD) of macroH2A1 or FAK, in line with the acquisition or loss of stemness features. In particular, macroH2A1 KD increased total sphingomyelin (SM) levels and decreased total lysophosphatidylcholine (LPC) levels, while FAK KD decreased total phosphatidylcholine (PC) levels. In conclusion, in HCC cell lines knocked down for specific signaling/epigenetic processes driving opposite stemness potential, we defined a lipidomic signature that hallmarks hepatic CSCs to be exploited for therapeutic strategies.
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spelling pubmed-77090392020-12-03 A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells Rivas Serna, Irma Magaly Romito, Ilaria Maugeri, Andrea Lo Re, Oriana Giallongo, Sebastiano Mazzoccoli, Gianluigi Oben, Jude A. Li Volti, Giovanni Mazza, Tommaso Alisi, Anna Vinciguerra, Manlio Int J Mol Sci Article Lipid catabolism and anabolism changes play a role in stemness acquisition by cancer cells, and cancer stem cells (CSCs) are particularly dependent on the activity of the enzymes involved in these processes. Lipidomic changes could play a role in CSCs’ ability to cause disease relapse and chemoresistance. The exploration of lipid composition and metabolism changes in CSCs in the context of hepatocellular cancer (HCC) is still incomplete and their lipidomic scenario continues to be elusive. We aimed to evaluate through high-throughput mass spectrometry (MS)-based lipidomics the levels of the members of the six major classes of sphingolipids and phospholipids in two HCC cell lines (HepG2 and Huh-7) silenced for the expression of histone variant macroH2A1 (favoring stemness acquisition), or silenced for the expression of focal adhesion tyrosine kinase (FAK) (hindering aggressiveness and stemness). Transcriptomic changes were evaluated by RNA sequencing as well. We found definite lipidomic and transcriptomic changes in the HCC lines upon knockdown (KD) of macroH2A1 or FAK, in line with the acquisition or loss of stemness features. In particular, macroH2A1 KD increased total sphingomyelin (SM) levels and decreased total lysophosphatidylcholine (LPC) levels, while FAK KD decreased total phosphatidylcholine (PC) levels. In conclusion, in HCC cell lines knocked down for specific signaling/epigenetic processes driving opposite stemness potential, we defined a lipidomic signature that hallmarks hepatic CSCs to be exploited for therapeutic strategies. MDPI 2020-11-10 /pmc/articles/PMC7709039/ /pubmed/33182805 http://dx.doi.org/10.3390/ijms21228452 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rivas Serna, Irma Magaly
Romito, Ilaria
Maugeri, Andrea
Lo Re, Oriana
Giallongo, Sebastiano
Mazzoccoli, Gianluigi
Oben, Jude A.
Li Volti, Giovanni
Mazza, Tommaso
Alisi, Anna
Vinciguerra, Manlio
A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells
title A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells
title_full A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells
title_fullStr A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells
title_full_unstemmed A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells
title_short A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells
title_sort lipidomic signature complements stemness features acquisition in liver cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709039/
https://www.ncbi.nlm.nih.gov/pubmed/33182805
http://dx.doi.org/10.3390/ijms21228452
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