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QTL Mapping for Resistance to Cankers Induced by Pseudomonas syringae pv. actinidiae (Psa) in a Tetraploid Actinidia chinensis Kiwifruit Population
Polyploidy is a key driver of significant evolutionary changes in plant species. The genus Actinidia (kiwifruit) exhibits multiple ploidy levels, which contribute to novel fruit traits, high yields and resistance to the canker-causing dieback disease incited by Pseudomonas syringae pv. actinidiae (P...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709049/ https://www.ncbi.nlm.nih.gov/pubmed/33233616 http://dx.doi.org/10.3390/pathogens9110967 |
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author | Tahir, Jibran Brendolise, Cyril Hoyte, Stephen Lucas, Marielle Thomson, Susan Hoeata, Kirsten McKenzie, Catherine Wotton, Andrew Funnell, Keith Morgan, Ed Hedderley, Duncan Chagné, David Bourke, Peter M. McCallum, John Gardiner, Susan E. Gea, Luis |
author_facet | Tahir, Jibran Brendolise, Cyril Hoyte, Stephen Lucas, Marielle Thomson, Susan Hoeata, Kirsten McKenzie, Catherine Wotton, Andrew Funnell, Keith Morgan, Ed Hedderley, Duncan Chagné, David Bourke, Peter M. McCallum, John Gardiner, Susan E. Gea, Luis |
author_sort | Tahir, Jibran |
collection | PubMed |
description | Polyploidy is a key driver of significant evolutionary changes in plant species. The genus Actinidia (kiwifruit) exhibits multiple ploidy levels, which contribute to novel fruit traits, high yields and resistance to the canker-causing dieback disease incited by Pseudomonas syringae pv. actinidiae (Psa) biovar 3. However, the genetic mechanism for resistance to Psa observed in polyploid kiwifruit is not yet known. In this study we performed detailed genetic analysis of a tetraploid Actinidia chinensis var. chinensis population derived from a cross between a female parent that exhibits weak tolerance to Psa and a highly Psa-resistant male parent. We used the capture-sequencing approach across the whole kiwifruit genome and generated the first ultra-dense maps in a tetraploid kiwifruit population. We located quantitative trait loci (QTLs) for Psa resistance on these maps. Our approach to QTL mapping is based on the use of identity-by-descent trait mapping, which allowed us to relate the contribution of specific alleles from their respective homologues in the male and female parent, to the control of Psa resistance in the progeny. We identified genes in the diploid reference genome whose function is suggested to be involved in plant defense, which underly the QTLs, including receptor-like kinases. Our study is the first to cast light on the genetics of a polyploid kiwifruit and suggest a plausible mechanism for Psa resistance in this species. |
format | Online Article Text |
id | pubmed-7709049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77090492020-12-03 QTL Mapping for Resistance to Cankers Induced by Pseudomonas syringae pv. actinidiae (Psa) in a Tetraploid Actinidia chinensis Kiwifruit Population Tahir, Jibran Brendolise, Cyril Hoyte, Stephen Lucas, Marielle Thomson, Susan Hoeata, Kirsten McKenzie, Catherine Wotton, Andrew Funnell, Keith Morgan, Ed Hedderley, Duncan Chagné, David Bourke, Peter M. McCallum, John Gardiner, Susan E. Gea, Luis Pathogens Article Polyploidy is a key driver of significant evolutionary changes in plant species. The genus Actinidia (kiwifruit) exhibits multiple ploidy levels, which contribute to novel fruit traits, high yields and resistance to the canker-causing dieback disease incited by Pseudomonas syringae pv. actinidiae (Psa) biovar 3. However, the genetic mechanism for resistance to Psa observed in polyploid kiwifruit is not yet known. In this study we performed detailed genetic analysis of a tetraploid Actinidia chinensis var. chinensis population derived from a cross between a female parent that exhibits weak tolerance to Psa and a highly Psa-resistant male parent. We used the capture-sequencing approach across the whole kiwifruit genome and generated the first ultra-dense maps in a tetraploid kiwifruit population. We located quantitative trait loci (QTLs) for Psa resistance on these maps. Our approach to QTL mapping is based on the use of identity-by-descent trait mapping, which allowed us to relate the contribution of specific alleles from their respective homologues in the male and female parent, to the control of Psa resistance in the progeny. We identified genes in the diploid reference genome whose function is suggested to be involved in plant defense, which underly the QTLs, including receptor-like kinases. Our study is the first to cast light on the genetics of a polyploid kiwifruit and suggest a plausible mechanism for Psa resistance in this species. MDPI 2020-11-20 /pmc/articles/PMC7709049/ /pubmed/33233616 http://dx.doi.org/10.3390/pathogens9110967 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tahir, Jibran Brendolise, Cyril Hoyte, Stephen Lucas, Marielle Thomson, Susan Hoeata, Kirsten McKenzie, Catherine Wotton, Andrew Funnell, Keith Morgan, Ed Hedderley, Duncan Chagné, David Bourke, Peter M. McCallum, John Gardiner, Susan E. Gea, Luis QTL Mapping for Resistance to Cankers Induced by Pseudomonas syringae pv. actinidiae (Psa) in a Tetraploid Actinidia chinensis Kiwifruit Population |
title | QTL Mapping for Resistance to Cankers Induced by Pseudomonas syringae pv. actinidiae (Psa) in a Tetraploid Actinidia chinensis Kiwifruit Population |
title_full | QTL Mapping for Resistance to Cankers Induced by Pseudomonas syringae pv. actinidiae (Psa) in a Tetraploid Actinidia chinensis Kiwifruit Population |
title_fullStr | QTL Mapping for Resistance to Cankers Induced by Pseudomonas syringae pv. actinidiae (Psa) in a Tetraploid Actinidia chinensis Kiwifruit Population |
title_full_unstemmed | QTL Mapping for Resistance to Cankers Induced by Pseudomonas syringae pv. actinidiae (Psa) in a Tetraploid Actinidia chinensis Kiwifruit Population |
title_short | QTL Mapping for Resistance to Cankers Induced by Pseudomonas syringae pv. actinidiae (Psa) in a Tetraploid Actinidia chinensis Kiwifruit Population |
title_sort | qtl mapping for resistance to cankers induced by pseudomonas syringae pv. actinidiae (psa) in a tetraploid actinidia chinensis kiwifruit population |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709049/ https://www.ncbi.nlm.nih.gov/pubmed/33233616 http://dx.doi.org/10.3390/pathogens9110967 |
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