A Novel Proximity Biotinylation Assay Based on the Self-Associating Split GFP1–10/11

Proximity biotinylation was developed to detect physiologically relevant protein–protein interactions in living cells. In this method, the protein of interest is tagged with a promiscuous biotin ligase, such as BioID or BioID2, which produces activated biotin that reacts with nearby proteins; these...

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Detalles Bibliográficos
Autores principales: Kesari, Aditi S., Aryal, Uma K., LaCount, Douglas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709110/
https://www.ncbi.nlm.nih.gov/pubmed/33276494
http://dx.doi.org/10.3390/proteomes8040037

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709110/
https://www.ncbi.nlm.nih.gov/pubmed/33276494
http://dx.doi.org/10.3390/proteomes8040037

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