Cargando…
Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M)
The oxidation of ascorbic acid by air oxygen and hydrogen peroxide in the presence of the copper-binding compound (cbc) from bacteria Methylococcus capsulatus (M) was studied. The rate constant of ascorbic acid oxidation by air oxygen in the presence of the copper complex with cbc from M. capsulatus...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709488/ https://www.ncbi.nlm.nih.gov/pubmed/33049942 http://dx.doi.org/10.3390/biomimetics5040048 |
_version_ | 1783617761108295680 |
---|---|
author | Avdeeva, Lidia V. Gvozdev, Rudolf I. |
author_facet | Avdeeva, Lidia V. Gvozdev, Rudolf I. |
author_sort | Avdeeva, Lidia V. |
collection | PubMed |
description | The oxidation of ascorbic acid by air oxygen and hydrogen peroxide in the presence of the copper-binding compound (cbc) from bacteria Methylococcus capsulatus (M) was studied. The rate constant of ascorbic acid oxidation by air oxygen in the presence of the copper complex with cbc from M. capsulatus (M) was shown to be 1.5 times higher than that of the noncatalytic reaction. The rate constant of ascorbic acid oxidation by hydrogen peroxide in the presence of the copper complex with cbc from M. capsulatus (M) decreased by almost one-third compared to the reaction in the absence of the copper complex with cbc. It was assumed that cbc can be involved in a multilevel system of antioxidant protection and can protect a bacterial cell from oxidation stress. Thus, the cbc is mimetic ascorbate oxidase in the oxidation of ascorbic acid by molecular oxygen. |
format | Online Article Text |
id | pubmed-7709488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77094882020-12-03 Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M) Avdeeva, Lidia V. Gvozdev, Rudolf I. Biomimetics (Basel) Article The oxidation of ascorbic acid by air oxygen and hydrogen peroxide in the presence of the copper-binding compound (cbc) from bacteria Methylococcus capsulatus (M) was studied. The rate constant of ascorbic acid oxidation by air oxygen in the presence of the copper complex with cbc from M. capsulatus (M) was shown to be 1.5 times higher than that of the noncatalytic reaction. The rate constant of ascorbic acid oxidation by hydrogen peroxide in the presence of the copper complex with cbc from M. capsulatus (M) decreased by almost one-third compared to the reaction in the absence of the copper complex with cbc. It was assumed that cbc can be involved in a multilevel system of antioxidant protection and can protect a bacterial cell from oxidation stress. Thus, the cbc is mimetic ascorbate oxidase in the oxidation of ascorbic acid by molecular oxygen. MDPI 2020-10-08 /pmc/articles/PMC7709488/ /pubmed/33049942 http://dx.doi.org/10.3390/biomimetics5040048 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Avdeeva, Lidia V. Gvozdev, Rudolf I. Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M) |
title | Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M) |
title_full | Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M) |
title_fullStr | Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M) |
title_full_unstemmed | Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M) |
title_short | Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M) |
title_sort | oxidation of l-ascorbic acid in the presence of the copper-binding compound from methanotrophic bacteria methylococcus capsulatus (m) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709488/ https://www.ncbi.nlm.nih.gov/pubmed/33049942 http://dx.doi.org/10.3390/biomimetics5040048 |
work_keys_str_mv | AT avdeevalidiav oxidationoflascorbicacidinthepresenceofthecopperbindingcompoundfrommethanotrophicbacteriamethylococcuscapsulatusm AT gvozdevrudolfi oxidationoflascorbicacidinthepresenceofthecopperbindingcompoundfrommethanotrophicbacteriamethylococcuscapsulatusm |