Cargando…

Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M)

The oxidation of ascorbic acid by air oxygen and hydrogen peroxide in the presence of the copper-binding compound (cbc) from bacteria Methylococcus capsulatus (M) was studied. The rate constant of ascorbic acid oxidation by air oxygen in the presence of the copper complex with cbc from M. capsulatus...

Descripción completa

Detalles Bibliográficos
Autores principales: Avdeeva, Lidia V., Gvozdev, Rudolf I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709488/
https://www.ncbi.nlm.nih.gov/pubmed/33049942
http://dx.doi.org/10.3390/biomimetics5040048
_version_ 1783617761108295680
author Avdeeva, Lidia V.
Gvozdev, Rudolf I.
author_facet Avdeeva, Lidia V.
Gvozdev, Rudolf I.
author_sort Avdeeva, Lidia V.
collection PubMed
description The oxidation of ascorbic acid by air oxygen and hydrogen peroxide in the presence of the copper-binding compound (cbc) from bacteria Methylococcus capsulatus (M) was studied. The rate constant of ascorbic acid oxidation by air oxygen in the presence of the copper complex with cbc from M. capsulatus (M) was shown to be 1.5 times higher than that of the noncatalytic reaction. The rate constant of ascorbic acid oxidation by hydrogen peroxide in the presence of the copper complex with cbc from M. capsulatus (M) decreased by almost one-third compared to the reaction in the absence of the copper complex with cbc. It was assumed that cbc can be involved in a multilevel system of antioxidant protection and can protect a bacterial cell from oxidation stress. Thus, the cbc is mimetic ascorbate oxidase in the oxidation of ascorbic acid by molecular oxygen.
format Online
Article
Text
id pubmed-7709488
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77094882020-12-03 Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M) Avdeeva, Lidia V. Gvozdev, Rudolf I. Biomimetics (Basel) Article The oxidation of ascorbic acid by air oxygen and hydrogen peroxide in the presence of the copper-binding compound (cbc) from bacteria Methylococcus capsulatus (M) was studied. The rate constant of ascorbic acid oxidation by air oxygen in the presence of the copper complex with cbc from M. capsulatus (M) was shown to be 1.5 times higher than that of the noncatalytic reaction. The rate constant of ascorbic acid oxidation by hydrogen peroxide in the presence of the copper complex with cbc from M. capsulatus (M) decreased by almost one-third compared to the reaction in the absence of the copper complex with cbc. It was assumed that cbc can be involved in a multilevel system of antioxidant protection and can protect a bacterial cell from oxidation stress. Thus, the cbc is mimetic ascorbate oxidase in the oxidation of ascorbic acid by molecular oxygen. MDPI 2020-10-08 /pmc/articles/PMC7709488/ /pubmed/33049942 http://dx.doi.org/10.3390/biomimetics5040048 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Avdeeva, Lidia V.
Gvozdev, Rudolf I.
Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M)
title Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M)
title_full Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M)
title_fullStr Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M)
title_full_unstemmed Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M)
title_short Oxidation of L-Ascorbic Acid in the Presence of the Copper-Binding Compound from Methanotrophic Bacteria Methylococcus capsulatus (M)
title_sort oxidation of l-ascorbic acid in the presence of the copper-binding compound from methanotrophic bacteria methylococcus capsulatus (m)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709488/
https://www.ncbi.nlm.nih.gov/pubmed/33049942
http://dx.doi.org/10.3390/biomimetics5040048
work_keys_str_mv AT avdeevalidiav oxidationoflascorbicacidinthepresenceofthecopperbindingcompoundfrommethanotrophicbacteriamethylococcuscapsulatusm
AT gvozdevrudolfi oxidationoflascorbicacidinthepresenceofthecopperbindingcompoundfrommethanotrophicbacteriamethylococcuscapsulatusm