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Polydopamine Antioxidant Hydrogels for Wound Healing Applications

Antioxidants are known to improve the wound healing process and are researched as a therapeutic strategy to treat chronic wounds. Dopamine is a known neurotransmitter with antioxidant properties that can be polymerized to form polydopamine (PDA). Herein, polydopamine is demonstrated as an antioxidan...

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Autores principales: O’Connor, Naphtali A., Syed, Abdulhaq, Wong, Madeline, Hicks, Josiah, Nunez, Greisly, Jitianu, Andrei, Siler, Zach, Peterson, Marnie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709666/
https://www.ncbi.nlm.nih.gov/pubmed/33142677
http://dx.doi.org/10.3390/gels6040039
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author O’Connor, Naphtali A.
Syed, Abdulhaq
Wong, Madeline
Hicks, Josiah
Nunez, Greisly
Jitianu, Andrei
Siler, Zach
Peterson, Marnie
author_facet O’Connor, Naphtali A.
Syed, Abdulhaq
Wong, Madeline
Hicks, Josiah
Nunez, Greisly
Jitianu, Andrei
Siler, Zach
Peterson, Marnie
author_sort O’Connor, Naphtali A.
collection PubMed
description Antioxidants are known to improve the wound healing process and are researched as a therapeutic strategy to treat chronic wounds. Dopamine is a known neurotransmitter with antioxidant properties that can be polymerized to form polydopamine (PDA). Herein, polydopamine is demonstrated as an antioxidant biomaterial. In prior work, we developed methodology to prepare hydrogels by crosslinking polysaccharides with polyamines via epichlorohydrin and NaOH. Using this previously developed methodology, dextran hydrogels crosslinked with polydopamine were prepared. Darkening of the gels indicated the increasing incorporation of polydopamine within the hydrogels. In addition to basic pH, polydopamine can be formed by reaction with polyethylene imine (PEI), which results in PEI-PDA copolymer. Dextran was similarly crosslinked with the PEI-PDA copolymer and resulted in sturdier, darker gels, which had more polydopamine incorporated. Hydrogel morphology and strength were dependent on the feed ratios of dopamine. Antioxidant activity of polydopamine containing hydrogel was confirmed and shown to be dependent on the amount of dopamine used in hydrogel synthesis. Hydrogels with 0.5 dopamine to dextran feed ratio scavenged 78.8% of radicals in a 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant assay while gels with no dopamine scavenged only 1.4% of radicals. An ex vivo wound healing assay showed considerable cell migration with the PEI-PDA containing hydrogel.
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spelling pubmed-77096662020-12-03 Polydopamine Antioxidant Hydrogels for Wound Healing Applications O’Connor, Naphtali A. Syed, Abdulhaq Wong, Madeline Hicks, Josiah Nunez, Greisly Jitianu, Andrei Siler, Zach Peterson, Marnie Gels Article Antioxidants are known to improve the wound healing process and are researched as a therapeutic strategy to treat chronic wounds. Dopamine is a known neurotransmitter with antioxidant properties that can be polymerized to form polydopamine (PDA). Herein, polydopamine is demonstrated as an antioxidant biomaterial. In prior work, we developed methodology to prepare hydrogels by crosslinking polysaccharides with polyamines via epichlorohydrin and NaOH. Using this previously developed methodology, dextran hydrogels crosslinked with polydopamine were prepared. Darkening of the gels indicated the increasing incorporation of polydopamine within the hydrogels. In addition to basic pH, polydopamine can be formed by reaction with polyethylene imine (PEI), which results in PEI-PDA copolymer. Dextran was similarly crosslinked with the PEI-PDA copolymer and resulted in sturdier, darker gels, which had more polydopamine incorporated. Hydrogel morphology and strength were dependent on the feed ratios of dopamine. Antioxidant activity of polydopamine containing hydrogel was confirmed and shown to be dependent on the amount of dopamine used in hydrogel synthesis. Hydrogels with 0.5 dopamine to dextran feed ratio scavenged 78.8% of radicals in a 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant assay while gels with no dopamine scavenged only 1.4% of radicals. An ex vivo wound healing assay showed considerable cell migration with the PEI-PDA containing hydrogel. MDPI 2020-10-31 /pmc/articles/PMC7709666/ /pubmed/33142677 http://dx.doi.org/10.3390/gels6040039 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
O’Connor, Naphtali A.
Syed, Abdulhaq
Wong, Madeline
Hicks, Josiah
Nunez, Greisly
Jitianu, Andrei
Siler, Zach
Peterson, Marnie
Polydopamine Antioxidant Hydrogels for Wound Healing Applications
title Polydopamine Antioxidant Hydrogels for Wound Healing Applications
title_full Polydopamine Antioxidant Hydrogels for Wound Healing Applications
title_fullStr Polydopamine Antioxidant Hydrogels for Wound Healing Applications
title_full_unstemmed Polydopamine Antioxidant Hydrogels for Wound Healing Applications
title_short Polydopamine Antioxidant Hydrogels for Wound Healing Applications
title_sort polydopamine antioxidant hydrogels for wound healing applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709666/
https://www.ncbi.nlm.nih.gov/pubmed/33142677
http://dx.doi.org/10.3390/gels6040039
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