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Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like Lesions

Yaws is a skin debilitating disease caused by Treponema pallidum subspecies pertenue with most cases reported in children. World Health Organization (WHO) aims at total eradication of this disease through mass treatment of suspected cases followed by an intensive follow-up program. However, effectiv...

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Autores principales: Frimpong, Michael, Simpson, Shirley Victoria, Ahor, Hubert Senanu, Agbanyo, Abigail, Gyabaah, Solomon, Agbavor, Bernadette, Amanor, Ivy Brago, Addo, Kennedy Kwasi, Böhlken-Fascher, Susanne, Kissenkötter, Jonas, Wahed, Ahmed Abd El, Phillips, Richard Odame
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709673/
https://www.ncbi.nlm.nih.gov/pubmed/33036234
http://dx.doi.org/10.3390/tropicalmed5040157
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author Frimpong, Michael
Simpson, Shirley Victoria
Ahor, Hubert Senanu
Agbanyo, Abigail
Gyabaah, Solomon
Agbavor, Bernadette
Amanor, Ivy Brago
Addo, Kennedy Kwasi
Böhlken-Fascher, Susanne
Kissenkötter, Jonas
Wahed, Ahmed Abd El
Phillips, Richard Odame
author_facet Frimpong, Michael
Simpson, Shirley Victoria
Ahor, Hubert Senanu
Agbanyo, Abigail
Gyabaah, Solomon
Agbavor, Bernadette
Amanor, Ivy Brago
Addo, Kennedy Kwasi
Böhlken-Fascher, Susanne
Kissenkötter, Jonas
Wahed, Ahmed Abd El
Phillips, Richard Odame
author_sort Frimpong, Michael
collection PubMed
description Yaws is a skin debilitating disease caused by Treponema pallidum subspecies pertenue with most cases reported in children. World Health Organization (WHO) aims at total eradication of this disease through mass treatment of suspected cases followed by an intensive follow-up program. However, effective diagnosis is pivotal in the successful implementation of this control program. Recombinase polymerase amplification (RPA), an isothermal nucleic acid amplification technique offers a wider range of differentiation of pathogens including those isolated from chronic skin ulcers with similar characteristics such as Haemophilus ducreyi (H. ducreyi). We have developed a RPA assay for the simultaneous detection of Treponema pallidum (T. pallidum) and H. ducreyi (TPHD-RPA). The assay demonstrated no cross-reaction with other pathogens and enable detection of T. pallidum and H. ducreyi within 15 min at 42 °C. The RPA assay was validated with 49 clinical samples from individuals confirmed to have yaws by serological tests. Comparing the developed assay with commercial multiplex real-time PCR, the assay demonstrated 94% and 95% sensitivity for T. pallidum and H. ducreyi, respectively and 100% specificity. This simple novel TPHD-RPA assay enables the rapid detection of both T. pallidum and H. ducreyi in yaws-like lesions. This test could support the yaws eradication efforts by ensuring reliable diagnosis, to enable monitoring of program success and planning of follow-up interventions at the community level.
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spelling pubmed-77096732020-12-03 Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like Lesions Frimpong, Michael Simpson, Shirley Victoria Ahor, Hubert Senanu Agbanyo, Abigail Gyabaah, Solomon Agbavor, Bernadette Amanor, Ivy Brago Addo, Kennedy Kwasi Böhlken-Fascher, Susanne Kissenkötter, Jonas Wahed, Ahmed Abd El Phillips, Richard Odame Trop Med Infect Dis Article Yaws is a skin debilitating disease caused by Treponema pallidum subspecies pertenue with most cases reported in children. World Health Organization (WHO) aims at total eradication of this disease through mass treatment of suspected cases followed by an intensive follow-up program. However, effective diagnosis is pivotal in the successful implementation of this control program. Recombinase polymerase amplification (RPA), an isothermal nucleic acid amplification technique offers a wider range of differentiation of pathogens including those isolated from chronic skin ulcers with similar characteristics such as Haemophilus ducreyi (H. ducreyi). We have developed a RPA assay for the simultaneous detection of Treponema pallidum (T. pallidum) and H. ducreyi (TPHD-RPA). The assay demonstrated no cross-reaction with other pathogens and enable detection of T. pallidum and H. ducreyi within 15 min at 42 °C. The RPA assay was validated with 49 clinical samples from individuals confirmed to have yaws by serological tests. Comparing the developed assay with commercial multiplex real-time PCR, the assay demonstrated 94% and 95% sensitivity for T. pallidum and H. ducreyi, respectively and 100% specificity. This simple novel TPHD-RPA assay enables the rapid detection of both T. pallidum and H. ducreyi in yaws-like lesions. This test could support the yaws eradication efforts by ensuring reliable diagnosis, to enable monitoring of program success and planning of follow-up interventions at the community level. MDPI 2020-10-06 /pmc/articles/PMC7709673/ /pubmed/33036234 http://dx.doi.org/10.3390/tropicalmed5040157 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Frimpong, Michael
Simpson, Shirley Victoria
Ahor, Hubert Senanu
Agbanyo, Abigail
Gyabaah, Solomon
Agbavor, Bernadette
Amanor, Ivy Brago
Addo, Kennedy Kwasi
Böhlken-Fascher, Susanne
Kissenkötter, Jonas
Wahed, Ahmed Abd El
Phillips, Richard Odame
Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like Lesions
title Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like Lesions
title_full Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like Lesions
title_fullStr Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like Lesions
title_full_unstemmed Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like Lesions
title_short Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like Lesions
title_sort multiplex recombinase polymerase amplification assay for simultaneous detection of treponema pallidum and haemophilus ducreyi in yaws-like lesions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709673/
https://www.ncbi.nlm.nih.gov/pubmed/33036234
http://dx.doi.org/10.3390/tropicalmed5040157
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