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circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells

Accumulating evidence has indicated that circular RNAs (circRNAs) serve crucial roles in the progression of a diverse range of different types of cancer, including osteosarcoma (OS). The present study determined the expression pattern and function of circRNA homeodomain interacting protein kinase 3...

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Autores principales: Wen, Yu, Li, Bin, He, Ming, Teng, Songling, Sun, Yuxiu, Wang, Guangbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709833/
https://www.ncbi.nlm.nih.gov/pubmed/33416147
http://dx.doi.org/10.3892/or.2020.7833
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author Wen, Yu
Li, Bin
He, Ming
Teng, Songling
Sun, Yuxiu
Wang, Guangbin
author_facet Wen, Yu
Li, Bin
He, Ming
Teng, Songling
Sun, Yuxiu
Wang, Guangbin
author_sort Wen, Yu
collection PubMed
description Accumulating evidence has indicated that circular RNAs (circRNAs) serve crucial roles in the progression of a diverse range of different types of cancer, including osteosarcoma (OS). The present study determined the expression pattern and function of circRNA homeodomain interacting protein kinase 3 (circHIPK3), a novel circular RNA, in OS. It was revealed that circHIPK3 expression was upregulated in OS tissue samples and OS cell lines. A localization assay revealed that circHIPK3 was primarily located in the cytoplasm. Using loss-of-function proliferation and Transwell assays, the present study revealed that circHIPK3-knockdown suppressed OS cell proliferation, migration and invasion. Furthermore, the present study screened potential microRNAs that may interact with circHIPK3. It was revealed that microRNA-637 (miR-637) expression was downregulated in OS according to a Gene Expression Omnibus data analysis. In addition, the present study demonstrated that miR-637 expression was downregulated in OS cell lines. A fluorescence in situ hybridization assay revealed that both miR-637 and circHIPK3 were located in the cytoplasm. An in-depth mechanism investigation demonstrated that circHIPK3 expression was inversely correlated with miR-637 expression, and that circHIPK3 was a target of miR-637. In addition, it was revealed that histone deacetylase 4 (HDAC4) was another downstream target gene of miR-637, as demonstrated using a luciferase assay. It was revealed that miR-637 suppressed OS cell proliferation, migration and invasion via targeting of HDAC4. Finally, the present study demonstrated that circHIPK3 sponged miR-637 to promote HDAC4 expression and OS cell proliferation, migration and invasion. In conclusion, the present study uncovered the role of the circHIPK3/miR-637/HDAC4 axis in OS cell proliferation, migration and invasion. It was demonstrated that circHIPK3 promoted OS cell proliferation, migration and invasion by modulating miR-637/HDAC4 signaling.
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spelling pubmed-77098332020-12-03 circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells Wen, Yu Li, Bin He, Ming Teng, Songling Sun, Yuxiu Wang, Guangbin Oncol Rep Articles Accumulating evidence has indicated that circular RNAs (circRNAs) serve crucial roles in the progression of a diverse range of different types of cancer, including osteosarcoma (OS). The present study determined the expression pattern and function of circRNA homeodomain interacting protein kinase 3 (circHIPK3), a novel circular RNA, in OS. It was revealed that circHIPK3 expression was upregulated in OS tissue samples and OS cell lines. A localization assay revealed that circHIPK3 was primarily located in the cytoplasm. Using loss-of-function proliferation and Transwell assays, the present study revealed that circHIPK3-knockdown suppressed OS cell proliferation, migration and invasion. Furthermore, the present study screened potential microRNAs that may interact with circHIPK3. It was revealed that microRNA-637 (miR-637) expression was downregulated in OS according to a Gene Expression Omnibus data analysis. In addition, the present study demonstrated that miR-637 expression was downregulated in OS cell lines. A fluorescence in situ hybridization assay revealed that both miR-637 and circHIPK3 were located in the cytoplasm. An in-depth mechanism investigation demonstrated that circHIPK3 expression was inversely correlated with miR-637 expression, and that circHIPK3 was a target of miR-637. In addition, it was revealed that histone deacetylase 4 (HDAC4) was another downstream target gene of miR-637, as demonstrated using a luciferase assay. It was revealed that miR-637 suppressed OS cell proliferation, migration and invasion via targeting of HDAC4. Finally, the present study demonstrated that circHIPK3 sponged miR-637 to promote HDAC4 expression and OS cell proliferation, migration and invasion. In conclusion, the present study uncovered the role of the circHIPK3/miR-637/HDAC4 axis in OS cell proliferation, migration and invasion. It was demonstrated that circHIPK3 promoted OS cell proliferation, migration and invasion by modulating miR-637/HDAC4 signaling. D.A. Spandidos 2021-01 2020-11-03 /pmc/articles/PMC7709833/ /pubmed/33416147 http://dx.doi.org/10.3892/or.2020.7833 Text en Copyright: © Wen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wen, Yu
Li, Bin
He, Ming
Teng, Songling
Sun, Yuxiu
Wang, Guangbin
circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells
title circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells
title_full circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells
title_fullStr circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells
title_full_unstemmed circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells
title_short circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells
title_sort circhipk3 promotes proliferation and migration and invasion via regulation of mir-637/hdac4 signaling in osteosarcoma cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709833/
https://www.ncbi.nlm.nih.gov/pubmed/33416147
http://dx.doi.org/10.3892/or.2020.7833
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