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ProAlanase is an Effective Alternative to Trypsin for Proteomics Applications and Disulfide Bond Mapping

Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of the digestion solution. In this study we characterize ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on t...

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Detalles Bibliográficos
Autores principales:	Samodova, Diana, Hosfield, Christopher M., Cramer, Christian N., Giuli, Maria V., Cappellini, Enrico, Franciosa, Giulia, Rosenblatt, Michael M., Kelstrup, Christian D., Olsen, Jesper V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The American Society for Biochemistry and Molecular Biology 2020
Materias:	Technological Innovation and Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710147/ https://www.ncbi.nlm.nih.gov/pubmed/33020190 http://dx.doi.org/10.1074/mcp.TIR120.002129

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