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Identification of urinary microRNA biomarkers for in vivo gentamicin-induced nephrotoxicity models
BACKGROUND: Although previous in vivo studies explored urinary microRNA (miRNA), there is no agreement on nephrotoxicity-specific miRNA biomarkers. OBJECTIVES: In this study, we assessed whether urinary miRNAs could be employed as biomarkers for nephrotoxicity. METHODS: For this, literature-based ca...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710462/ https://www.ncbi.nlm.nih.gov/pubmed/33263228 http://dx.doi.org/10.4142/jvs.2020.21.e81 |
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author | Jeon, Byung-Suk Lee, Soo-ho Hwang, So-Ryeon Yi, Hee Bang, Ji-Hyun Tham, Nga Thi Thu Lee, Hyun-Kyoung Woo, Gye-Hyeong Kang, Hwan-Goo Ku, Hyun-Ok |
author_facet | Jeon, Byung-Suk Lee, Soo-ho Hwang, So-Ryeon Yi, Hee Bang, Ji-Hyun Tham, Nga Thi Thu Lee, Hyun-Kyoung Woo, Gye-Hyeong Kang, Hwan-Goo Ku, Hyun-Ok |
author_sort | Jeon, Byung-Suk |
collection | PubMed |
description | BACKGROUND: Although previous in vivo studies explored urinary microRNA (miRNA), there is no agreement on nephrotoxicity-specific miRNA biomarkers. OBJECTIVES: In this study, we assessed whether urinary miRNAs could be employed as biomarkers for nephrotoxicity. METHODS: For this, literature-based candidate miRNAs were identified by reviewing the previous studies. Female Sprague-Dawley rats received subcutaneous injections of a single dose or repeated doses (3 consecutive days) of gentamicin (GEN; 137 or 412 mg/kg). The expression of miRNAs was analyzed by real-time reverse transcription-polymerase chain reaction in 16 h pooled urine from GEN-treated rats. RESULTS: GEN-induced acute kidney injury was confirmed by the presence of tubular necrosis. We identified let-7g-5p, miR-21-3p, 26b-3p, 192-5p, and 378a-3p significantly upregulated in the urine of GEN-treated rats with the appearance of the necrosis in proximal tubules. Specifically, miR-26-3p, 192-5p, and 378a-3p with highly expressed levels in urine of rats with GEN-induced acute tubular injury were considered to have sensitivities comparable to clinical biomarkers, such as blood urea nitrogen, serum creatinine, and urinary kidney injury molecule protein. CONCLUSIONS: These results indicated the potential involvement of urinary miRNAs in chemical-induced nephrotoxicity, suggesting that certain miRNAs could serve as biomarkers for acute nephrotoxicity. |
format | Online Article Text |
id | pubmed-7710462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-77104622020-12-08 Identification of urinary microRNA biomarkers for in vivo gentamicin-induced nephrotoxicity models Jeon, Byung-Suk Lee, Soo-ho Hwang, So-Ryeon Yi, Hee Bang, Ji-Hyun Tham, Nga Thi Thu Lee, Hyun-Kyoung Woo, Gye-Hyeong Kang, Hwan-Goo Ku, Hyun-Ok J Vet Sci Original Article BACKGROUND: Although previous in vivo studies explored urinary microRNA (miRNA), there is no agreement on nephrotoxicity-specific miRNA biomarkers. OBJECTIVES: In this study, we assessed whether urinary miRNAs could be employed as biomarkers for nephrotoxicity. METHODS: For this, literature-based candidate miRNAs were identified by reviewing the previous studies. Female Sprague-Dawley rats received subcutaneous injections of a single dose or repeated doses (3 consecutive days) of gentamicin (GEN; 137 or 412 mg/kg). The expression of miRNAs was analyzed by real-time reverse transcription-polymerase chain reaction in 16 h pooled urine from GEN-treated rats. RESULTS: GEN-induced acute kidney injury was confirmed by the presence of tubular necrosis. We identified let-7g-5p, miR-21-3p, 26b-3p, 192-5p, and 378a-3p significantly upregulated in the urine of GEN-treated rats with the appearance of the necrosis in proximal tubules. Specifically, miR-26-3p, 192-5p, and 378a-3p with highly expressed levels in urine of rats with GEN-induced acute tubular injury were considered to have sensitivities comparable to clinical biomarkers, such as blood urea nitrogen, serum creatinine, and urinary kidney injury molecule protein. CONCLUSIONS: These results indicated the potential involvement of urinary miRNAs in chemical-induced nephrotoxicity, suggesting that certain miRNAs could serve as biomarkers for acute nephrotoxicity. The Korean Society of Veterinary Science 2020-11 2020-10-05 /pmc/articles/PMC7710462/ /pubmed/33263228 http://dx.doi.org/10.4142/jvs.2020.21.e81 Text en © 2020 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jeon, Byung-Suk Lee, Soo-ho Hwang, So-Ryeon Yi, Hee Bang, Ji-Hyun Tham, Nga Thi Thu Lee, Hyun-Kyoung Woo, Gye-Hyeong Kang, Hwan-Goo Ku, Hyun-Ok Identification of urinary microRNA biomarkers for in vivo gentamicin-induced nephrotoxicity models |
title | Identification of urinary microRNA biomarkers for in vivo gentamicin-induced nephrotoxicity models |
title_full | Identification of urinary microRNA biomarkers for in vivo gentamicin-induced nephrotoxicity models |
title_fullStr | Identification of urinary microRNA biomarkers for in vivo gentamicin-induced nephrotoxicity models |
title_full_unstemmed | Identification of urinary microRNA biomarkers for in vivo gentamicin-induced nephrotoxicity models |
title_short | Identification of urinary microRNA biomarkers for in vivo gentamicin-induced nephrotoxicity models |
title_sort | identification of urinary microrna biomarkers for in vivo gentamicin-induced nephrotoxicity models |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710462/ https://www.ncbi.nlm.nih.gov/pubmed/33263228 http://dx.doi.org/10.4142/jvs.2020.21.e81 |
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