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Disposable silicon-based all-in-one micro-qPCR for apid on-site detection of pathogens

Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or sim...

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Detalles Bibliográficos
Autores principales: Nunez-Bajo, Estefania, Silva Pinto Collins, Alexander, Kasimatis, Michael, Cotur, Yasin, Asfour, Tarek, Tanriverdi, Ugur, Grell, Max, Kaisti, Matti, Senesi, Guglielmo, Stevenson, Karen, Güder, Firat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710731/
https://www.ncbi.nlm.nih.gov/pubmed/33268779
http://dx.doi.org/10.1038/s41467-020-19911-6
Descripción
Sumario:Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences of nucleic acids. TriSilix can be produced at wafer-scale in a standard laboratory (37 chips of 10 × 10 × 0.65 mm in size can be produced in 7 h, costing ~0.35 USD per device). We are able to quantitatively detect a 563 bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20 fg, equivalent to a single bacterium, at the 35(th) cycle. Using TriSilix, we also detect the cDNA from SARS-CoV-2 (1 pg) with high specificity against SARS-CoV (2003).