Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations

BACKGROUND: Plasmid propagation in recombination strains such as Escherichia coli DH5α is regarded as a beneficial instrument for stable amplification of the DNA materials. Here, we show trans-conjugation of pGEM-T cloning vector (modified Promega PCR product cloning vector with tra genes, transposa...

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Detalles Bibliográficos
Autores principales: Hamed, Ahmed A., Khedr, Mohamed, Abdelraof, Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710787/
https://www.ncbi.nlm.nih.gov/pubmed/33263861
http://dx.doi.org/10.1186/s43141-020-00096-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710787/
https://www.ncbi.nlm.nih.gov/pubmed/33263861
http://dx.doi.org/10.1186/s43141-020-00096-w

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