Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice

Anti-aging research suggests that immunosenescent cells can play deleterious roles in the immune system. Here, young (2 months old) and old (14 months old) C57BL/6 mice received a daily oral dose (100 or 750 mg/kg/day) of acid-hydrolyzed silk peptide (SP) for a period of 5 weeks. Mouse spleen, lymph...

Descripción completa

Detalles Bibliográficos
Autores principales: Chei, Sungwoo, Oh, Hyun-Ji, Lee, Kippeum, Jin, Heegu, Lee, Jeong-Yong, Lee, Boo-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Nutrition
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710868/
https://www.ncbi.nlm.nih.gov/pubmed/33330583
http://dx.doi.org/10.3389/fnut.2020.583186
_version_ 1783618024412020736
author Chei, Sungwoo
Oh, Hyun-Ji
Lee, Kippeum
Jin, Heegu
Lee, Jeong-Yong
Lee, Boo-Yong
author_facet Chei, Sungwoo
Oh, Hyun-Ji
Lee, Kippeum
Jin, Heegu
Lee, Jeong-Yong
Lee, Boo-Yong
author_sort Chei, Sungwoo
collection PubMed
description Anti-aging research suggests that immunosenescent cells can play deleterious roles in the immune system. Here, young (2 months old) and old (14 months old) C57BL/6 mice received a daily oral dose (100 or 750 mg/kg/day) of acid-hydrolyzed silk peptide (SP) for a period of 5 weeks. Mouse spleen, lymph node, and serum were analyzed to determine the immune homeostasis of SP by flow cytometry, Western blotting, ELISA, and qRT-PCR. The results suggest that SP ameliorates age-related dysfunction of T and B cells. Amelioration of B cell dysfunction improved the immunoglobulin response in aged mice. Taken together, the results suggest that SP restores immune homeostasis with respect to immunosenescent cells.
format Online
Article
Text
id pubmed-7710868
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-77108682020-12-15 Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice Chei, Sungwoo Oh, Hyun-Ji Lee, Kippeum Jin, Heegu Lee, Jeong-Yong Lee, Boo-Yong Front Nutr Nutrition Anti-aging research suggests that immunosenescent cells can play deleterious roles in the immune system. Here, young (2 months old) and old (14 months old) C57BL/6 mice received a daily oral dose (100 or 750 mg/kg/day) of acid-hydrolyzed silk peptide (SP) for a period of 5 weeks. Mouse spleen, lymph node, and serum were analyzed to determine the immune homeostasis of SP by flow cytometry, Western blotting, ELISA, and qRT-PCR. The results suggest that SP ameliorates age-related dysfunction of T and B cells. Amelioration of B cell dysfunction improved the immunoglobulin response in aged mice. Taken together, the results suggest that SP restores immune homeostasis with respect to immunosenescent cells. Frontiers Media S.A. 2020-11-19 /pmc/articles/PMC7710868/ /pubmed/33330583 http://dx.doi.org/10.3389/fnut.2020.583186 Text en Copyright © 2020 Chei, Oh, Lee, Jin, Lee and Lee. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Chei, Sungwoo
Oh, Hyun-Ji
Lee, Kippeum
Jin, Heegu
Lee, Jeong-Yong
Lee, Boo-Yong
Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice
title Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice
title_full Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice
title_fullStr Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice
title_full_unstemmed Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice
title_short Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice
title_sort dysfunction of b cell leading to failure of immunoglobulin response is ameliorated by dietary silk peptide in 14-month-old c57bl/6 mice
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710868/
https://www.ncbi.nlm.nih.gov/pubmed/33330583
http://dx.doi.org/10.3389/fnut.2020.583186
work_keys_str_mv AT cheisungwoo dysfunctionofbcellleadingtofailureofimmunoglobulinresponseisamelioratedbydietarysilkpeptidein14montholdc57bl6mice
AT ohhyunji dysfunctionofbcellleadingtofailureofimmunoglobulinresponseisamelioratedbydietarysilkpeptidein14montholdc57bl6mice
AT leekippeum dysfunctionofbcellleadingtofailureofimmunoglobulinresponseisamelioratedbydietarysilkpeptidein14montholdc57bl6mice
AT jinheegu dysfunctionofbcellleadingtofailureofimmunoglobulinresponseisamelioratedbydietarysilkpeptidein14montholdc57bl6mice
AT leejeongyong dysfunctionofbcellleadingtofailureofimmunoglobulinresponseisamelioratedbydietarysilkpeptidein14montholdc57bl6mice
AT leebooyong dysfunctionofbcellleadingtofailureofimmunoglobulinresponseisamelioratedbydietarysilkpeptidein14montholdc57bl6mice

Ejemplares similares