Cargando…

Rapid Scanning Electron Microscopy Detection and Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 and Other Respiratory Viruses

There is an urgent need for accurate and rapid testing methods to quickly identify infected patients as well as asymptomatic carriers, in order to prevent the spread of emerging viruses. Here, we developed a rapid testing strategy by scanning electron microscopy capable of detecting severe acute res...

Descripción completa

Detalles Bibliográficos
Autores principales: Haddad, Gabriel, Bellali, Sara, Fontanini, Anthony, Francis, Rania, La Scola, Bernard, Levasseur, Anthony, Bou Khalil, Jacques, Raoult, Didier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711091/
https://www.ncbi.nlm.nih.gov/pubmed/33329483
http://dx.doi.org/10.3389/fmicb.2020.596180
_version_ 1783618071570677760
author Haddad, Gabriel
Bellali, Sara
Fontanini, Anthony
Francis, Rania
La Scola, Bernard
Levasseur, Anthony
Bou Khalil, Jacques
Raoult, Didier
author_facet Haddad, Gabriel
Bellali, Sara
Fontanini, Anthony
Francis, Rania
La Scola, Bernard
Levasseur, Anthony
Bou Khalil, Jacques
Raoult, Didier
author_sort Haddad, Gabriel
collection PubMed
description There is an urgent need for accurate and rapid testing methods to quickly identify infected patients as well as asymptomatic carriers, in order to prevent the spread of emerging viruses. Here, we developed a rapid testing strategy by scanning electron microscopy capable of detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other respiratory viruses directly from patients. We evaluated our results by comparing them to real-time reverse transcription-polymerase chain reaction (RT-PCR) and metagenomic sequencing results. We correlated the presence of the SARS-CoV-2 to the viral load, where samples with Ct values lower than 18 were all detected by scanning electron microscopy (SEM). The sensitivity deacresed progressively with higher Ct values. In addition, we found a correlation with metagenomic sequencing, where all samples detected by SEM were sequenced and viral sequences were easily recovered. Following this study, SEM proved its efficiency as a frontline method for directly detecting previously unknown microorganisms that cannot be targeted by molecular methods and can cause potential outbreaks.
format Online
Article
Text
id pubmed-7711091
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-77110912020-12-15 Rapid Scanning Electron Microscopy Detection and Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 and Other Respiratory Viruses Haddad, Gabriel Bellali, Sara Fontanini, Anthony Francis, Rania La Scola, Bernard Levasseur, Anthony Bou Khalil, Jacques Raoult, Didier Front Microbiol Microbiology There is an urgent need for accurate and rapid testing methods to quickly identify infected patients as well as asymptomatic carriers, in order to prevent the spread of emerging viruses. Here, we developed a rapid testing strategy by scanning electron microscopy capable of detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other respiratory viruses directly from patients. We evaluated our results by comparing them to real-time reverse transcription-polymerase chain reaction (RT-PCR) and metagenomic sequencing results. We correlated the presence of the SARS-CoV-2 to the viral load, where samples with Ct values lower than 18 were all detected by scanning electron microscopy (SEM). The sensitivity deacresed progressively with higher Ct values. In addition, we found a correlation with metagenomic sequencing, where all samples detected by SEM were sequenced and viral sequences were easily recovered. Following this study, SEM proved its efficiency as a frontline method for directly detecting previously unknown microorganisms that cannot be targeted by molecular methods and can cause potential outbreaks. Frontiers Media S.A. 2020-11-19 /pmc/articles/PMC7711091/ /pubmed/33329483 http://dx.doi.org/10.3389/fmicb.2020.596180 Text en Copyright © 2020 Haddad, Bellali, Fontanini, Francis, La Scola, Levasseur, Bou Khalil and Raoult. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Haddad, Gabriel
Bellali, Sara
Fontanini, Anthony
Francis, Rania
La Scola, Bernard
Levasseur, Anthony
Bou Khalil, Jacques
Raoult, Didier
Rapid Scanning Electron Microscopy Detection and Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 and Other Respiratory Viruses
title Rapid Scanning Electron Microscopy Detection and Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 and Other Respiratory Viruses
title_full Rapid Scanning Electron Microscopy Detection and Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 and Other Respiratory Viruses
title_fullStr Rapid Scanning Electron Microscopy Detection and Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 and Other Respiratory Viruses
title_full_unstemmed Rapid Scanning Electron Microscopy Detection and Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 and Other Respiratory Viruses
title_short Rapid Scanning Electron Microscopy Detection and Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 and Other Respiratory Viruses
title_sort rapid scanning electron microscopy detection and sequencing of severe acute respiratory syndrome coronavirus 2 and other respiratory viruses
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711091/
https://www.ncbi.nlm.nih.gov/pubmed/33329483
http://dx.doi.org/10.3389/fmicb.2020.596180
work_keys_str_mv AT haddadgabriel rapidscanningelectronmicroscopydetectionandsequencingofsevereacuterespiratorysyndromecoronavirus2andotherrespiratoryviruses
AT bellalisara rapidscanningelectronmicroscopydetectionandsequencingofsevereacuterespiratorysyndromecoronavirus2andotherrespiratoryviruses
AT fontaninianthony rapidscanningelectronmicroscopydetectionandsequencingofsevereacuterespiratorysyndromecoronavirus2andotherrespiratoryviruses
AT francisrania rapidscanningelectronmicroscopydetectionandsequencingofsevereacuterespiratorysyndromecoronavirus2andotherrespiratoryviruses
AT lascolabernard rapidscanningelectronmicroscopydetectionandsequencingofsevereacuterespiratorysyndromecoronavirus2andotherrespiratoryviruses
AT levasseuranthony rapidscanningelectronmicroscopydetectionandsequencingofsevereacuterespiratorysyndromecoronavirus2andotherrespiratoryviruses
AT boukhaliljacques rapidscanningelectronmicroscopydetectionandsequencingofsevereacuterespiratorysyndromecoronavirus2andotherrespiratoryviruses
AT raoultdidier rapidscanningelectronmicroscopydetectionandsequencingofsevereacuterespiratorysyndromecoronavirus2andotherrespiratoryviruses