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Protective multi-epitope candidate vaccine for urinary tract infection

Urinary tract infections (UTIs) are induced by exogenous organisms including extraintestinal pathogenic such as Escherichia coli (ExPEC), Proteus mirabilis and Klebsiella pneumonia, which are closely related. These organisms can colonize in the urinary tract and cause UTIs. In this study, a cross-re...

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Autores principales: Oloomi, Mana, Javadi, Maryam, Asadi Karam, Mohammad Reza, Khezerloo, Jamil Kheirvari, Haghri, Zohreh, Bouzari, Saeid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711219/
https://www.ncbi.nlm.nih.gov/pubmed/33304840
http://dx.doi.org/10.1016/j.btre.2020.e00564
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author Oloomi, Mana
Javadi, Maryam
Asadi Karam, Mohammad Reza
Khezerloo, Jamil Kheirvari
Haghri, Zohreh
Bouzari, Saeid
author_facet Oloomi, Mana
Javadi, Maryam
Asadi Karam, Mohammad Reza
Khezerloo, Jamil Kheirvari
Haghri, Zohreh
Bouzari, Saeid
author_sort Oloomi, Mana
collection PubMed
description Urinary tract infections (UTIs) are induced by exogenous organisms including extraintestinal pathogenic such as Escherichia coli (ExPEC), Proteus mirabilis and Klebsiella pneumonia, which are closely related. These organisms can colonize in the urinary tract and cause UTIs. In this study, a cross-reactive multi-epitope vaccine was designed by two constructs to stimulate the immune system (CD8+ and CD4 + T cells) against ExPEC, Proteus mirabilis and Klebsiella pneumonia strains. Uropathogenic Escherichia coli (UPEC), Proteus mirabilis and Klebsiella pneumoniae are the main bacterial cause of UTI. They were used for designing experimental candidate vaccine, and their immunogenicity and protectivity were assessed. In this study, conserved antigens from their bacterial genomes were considered, and informatics-based immunological vaccine with cross-protective T and B-cells epitopes was designed and evaluated. The vaccine candidate was used as a broad immune system inducer, and its cross-protective immunity and protectivity were confirmed in in vivo experiments.
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spelling pubmed-77112192020-12-09 Protective multi-epitope candidate vaccine for urinary tract infection Oloomi, Mana Javadi, Maryam Asadi Karam, Mohammad Reza Khezerloo, Jamil Kheirvari Haghri, Zohreh Bouzari, Saeid Biotechnol Rep (Amst) Research Article Urinary tract infections (UTIs) are induced by exogenous organisms including extraintestinal pathogenic such as Escherichia coli (ExPEC), Proteus mirabilis and Klebsiella pneumonia, which are closely related. These organisms can colonize in the urinary tract and cause UTIs. In this study, a cross-reactive multi-epitope vaccine was designed by two constructs to stimulate the immune system (CD8+ and CD4 + T cells) against ExPEC, Proteus mirabilis and Klebsiella pneumonia strains. Uropathogenic Escherichia coli (UPEC), Proteus mirabilis and Klebsiella pneumoniae are the main bacterial cause of UTI. They were used for designing experimental candidate vaccine, and their immunogenicity and protectivity were assessed. In this study, conserved antigens from their bacterial genomes were considered, and informatics-based immunological vaccine with cross-protective T and B-cells epitopes was designed and evaluated. The vaccine candidate was used as a broad immune system inducer, and its cross-protective immunity and protectivity were confirmed in in vivo experiments. Elsevier 2020-11-26 /pmc/articles/PMC7711219/ /pubmed/33304840 http://dx.doi.org/10.1016/j.btre.2020.e00564 Text en © 2020 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Oloomi, Mana
Javadi, Maryam
Asadi Karam, Mohammad Reza
Khezerloo, Jamil Kheirvari
Haghri, Zohreh
Bouzari, Saeid
Protective multi-epitope candidate vaccine for urinary tract infection
title Protective multi-epitope candidate vaccine for urinary tract infection
title_full Protective multi-epitope candidate vaccine for urinary tract infection
title_fullStr Protective multi-epitope candidate vaccine for urinary tract infection
title_full_unstemmed Protective multi-epitope candidate vaccine for urinary tract infection
title_short Protective multi-epitope candidate vaccine for urinary tract infection
title_sort protective multi-epitope candidate vaccine for urinary tract infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711219/
https://www.ncbi.nlm.nih.gov/pubmed/33304840
http://dx.doi.org/10.1016/j.btre.2020.e00564
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