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Protective multi-epitope candidate vaccine for urinary tract infection
Urinary tract infections (UTIs) are induced by exogenous organisms including extraintestinal pathogenic such as Escherichia coli (ExPEC), Proteus mirabilis and Klebsiella pneumonia, which are closely related. These organisms can colonize in the urinary tract and cause UTIs. In this study, a cross-re...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711219/ https://www.ncbi.nlm.nih.gov/pubmed/33304840 http://dx.doi.org/10.1016/j.btre.2020.e00564 |
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author | Oloomi, Mana Javadi, Maryam Asadi Karam, Mohammad Reza Khezerloo, Jamil Kheirvari Haghri, Zohreh Bouzari, Saeid |
author_facet | Oloomi, Mana Javadi, Maryam Asadi Karam, Mohammad Reza Khezerloo, Jamil Kheirvari Haghri, Zohreh Bouzari, Saeid |
author_sort | Oloomi, Mana |
collection | PubMed |
description | Urinary tract infections (UTIs) are induced by exogenous organisms including extraintestinal pathogenic such as Escherichia coli (ExPEC), Proteus mirabilis and Klebsiella pneumonia, which are closely related. These organisms can colonize in the urinary tract and cause UTIs. In this study, a cross-reactive multi-epitope vaccine was designed by two constructs to stimulate the immune system (CD8+ and CD4 + T cells) against ExPEC, Proteus mirabilis and Klebsiella pneumonia strains. Uropathogenic Escherichia coli (UPEC), Proteus mirabilis and Klebsiella pneumoniae are the main bacterial cause of UTI. They were used for designing experimental candidate vaccine, and their immunogenicity and protectivity were assessed. In this study, conserved antigens from their bacterial genomes were considered, and informatics-based immunological vaccine with cross-protective T and B-cells epitopes was designed and evaluated. The vaccine candidate was used as a broad immune system inducer, and its cross-protective immunity and protectivity were confirmed in in vivo experiments. |
format | Online Article Text |
id | pubmed-7711219 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77112192020-12-09 Protective multi-epitope candidate vaccine for urinary tract infection Oloomi, Mana Javadi, Maryam Asadi Karam, Mohammad Reza Khezerloo, Jamil Kheirvari Haghri, Zohreh Bouzari, Saeid Biotechnol Rep (Amst) Research Article Urinary tract infections (UTIs) are induced by exogenous organisms including extraintestinal pathogenic such as Escherichia coli (ExPEC), Proteus mirabilis and Klebsiella pneumonia, which are closely related. These organisms can colonize in the urinary tract and cause UTIs. In this study, a cross-reactive multi-epitope vaccine was designed by two constructs to stimulate the immune system (CD8+ and CD4 + T cells) against ExPEC, Proteus mirabilis and Klebsiella pneumonia strains. Uropathogenic Escherichia coli (UPEC), Proteus mirabilis and Klebsiella pneumoniae are the main bacterial cause of UTI. They were used for designing experimental candidate vaccine, and their immunogenicity and protectivity were assessed. In this study, conserved antigens from their bacterial genomes were considered, and informatics-based immunological vaccine with cross-protective T and B-cells epitopes was designed and evaluated. The vaccine candidate was used as a broad immune system inducer, and its cross-protective immunity and protectivity were confirmed in in vivo experiments. Elsevier 2020-11-26 /pmc/articles/PMC7711219/ /pubmed/33304840 http://dx.doi.org/10.1016/j.btre.2020.e00564 Text en © 2020 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Oloomi, Mana Javadi, Maryam Asadi Karam, Mohammad Reza Khezerloo, Jamil Kheirvari Haghri, Zohreh Bouzari, Saeid Protective multi-epitope candidate vaccine for urinary tract infection |
title | Protective multi-epitope candidate vaccine for urinary tract infection |
title_full | Protective multi-epitope candidate vaccine for urinary tract infection |
title_fullStr | Protective multi-epitope candidate vaccine for urinary tract infection |
title_full_unstemmed | Protective multi-epitope candidate vaccine for urinary tract infection |
title_short | Protective multi-epitope candidate vaccine for urinary tract infection |
title_sort | protective multi-epitope candidate vaccine for urinary tract infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711219/ https://www.ncbi.nlm.nih.gov/pubmed/33304840 http://dx.doi.org/10.1016/j.btre.2020.e00564 |
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