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Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice

Non-typhoidal Salmonella (NTS) serovars are significant health burden worldwide. Although much effort has been devoted to developing typhoid-based vaccines for humans, currently there is no NTS vaccine available. Presented here is the efficacy of a live attenuated serovar Typhimurium strain (NC983)....

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Detalles Bibliográficos
Autores principales: Troxell, Bryan, Mendoza, Mary, Ali, Rizwana, Koci, Matthew, Hassan, Hosni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711481/
https://www.ncbi.nlm.nih.gov/pubmed/33153043
http://dx.doi.org/10.3390/vaccines8040646
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author Troxell, Bryan
Mendoza, Mary
Ali, Rizwana
Koci, Matthew
Hassan, Hosni
author_facet Troxell, Bryan
Mendoza, Mary
Ali, Rizwana
Koci, Matthew
Hassan, Hosni
author_sort Troxell, Bryan
collection PubMed
description Non-typhoidal Salmonella (NTS) serovars are significant health burden worldwide. Although much effort has been devoted to developing typhoid-based vaccines for humans, currently there is no NTS vaccine available. Presented here is the efficacy of a live attenuated serovar Typhimurium strain (NC983). Oral delivery of strain NC983 was capable of fully protecting C57BL/6 and BALB/c mice against challenge with virulent Typhimurium. Strain NC983 was found to elicit an anti-Typhimurium IgG response following administration of vaccine and boosting doses. Furthermore, in competition experiments with virulent S. Typhimurium (ATCC 14028), NC983 was highly defective in colonization of the murine liver and spleen. Collectively, these results indicate that strain NC983 is a potential live attenuated vaccine strain that warrants further development.
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spelling pubmed-77114812020-12-04 Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice Troxell, Bryan Mendoza, Mary Ali, Rizwana Koci, Matthew Hassan, Hosni Vaccines (Basel) Article Non-typhoidal Salmonella (NTS) serovars are significant health burden worldwide. Although much effort has been devoted to developing typhoid-based vaccines for humans, currently there is no NTS vaccine available. Presented here is the efficacy of a live attenuated serovar Typhimurium strain (NC983). Oral delivery of strain NC983 was capable of fully protecting C57BL/6 and BALB/c mice against challenge with virulent Typhimurium. Strain NC983 was found to elicit an anti-Typhimurium IgG response following administration of vaccine and boosting doses. Furthermore, in competition experiments with virulent S. Typhimurium (ATCC 14028), NC983 was highly defective in colonization of the murine liver and spleen. Collectively, these results indicate that strain NC983 is a potential live attenuated vaccine strain that warrants further development. MDPI 2020-11-03 /pmc/articles/PMC7711481/ /pubmed/33153043 http://dx.doi.org/10.3390/vaccines8040646 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Troxell, Bryan
Mendoza, Mary
Ali, Rizwana
Koci, Matthew
Hassan, Hosni
Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice
title Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice
title_full Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice
title_fullStr Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice
title_full_unstemmed Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice
title_short Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice
title_sort attenuated salmonella enterica serovar typhimurium, strain nc983, is immunogenic, and protective against virulent typhimurium challenges in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711481/
https://www.ncbi.nlm.nih.gov/pubmed/33153043
http://dx.doi.org/10.3390/vaccines8040646
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