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Pullulan-Coated Iron Oxide Nanoparticles for Blood-Stage Malaria Vaccine Delivery

Vaccines against blood-stage malaria often aim to induce antibodies to neutralize parasite entry into red blood cells, interferon gamma (IFNγ) produced by T helper 1 (Th1) CD4+ T cells or interleukin 4 (IL-4) produced by T helper 2 (Th2) cells to provide B cell help. One vaccine delivery method for...

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Detalles Bibliográficos
Autores principales: Powles, Liam, Wilson, Kirsty L., Xiang, Sue D., Coppel, Ross L., Ma, Charles, Selomulya, Cordelia, Plebanski, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711541/
https://www.ncbi.nlm.nih.gov/pubmed/33153189
http://dx.doi.org/10.3390/vaccines8040651
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author Powles, Liam
Wilson, Kirsty L.
Xiang, Sue D.
Coppel, Ross L.
Ma, Charles
Selomulya, Cordelia
Plebanski, Magdalena
author_facet Powles, Liam
Wilson, Kirsty L.
Xiang, Sue D.
Coppel, Ross L.
Ma, Charles
Selomulya, Cordelia
Plebanski, Magdalena
author_sort Powles, Liam
collection PubMed
description Vaccines against blood-stage malaria often aim to induce antibodies to neutralize parasite entry into red blood cells, interferon gamma (IFNγ) produced by T helper 1 (Th1) CD4+ T cells or interleukin 4 (IL-4) produced by T helper 2 (Th2) cells to provide B cell help. One vaccine delivery method for suitable putative malaria protein antigens is the use of nanoparticles as vaccine carriers. It has been previously shown that antigen conjugated to inorganic nanoparticles in the viral-particle size range (~40–60 nm) can induce protective antibodies and T cells against malaria antigens in a rodent malaria challenge model. Herein, it is shown that biodegradable pullulan-coated iron oxide nanoparticles (pIONPs) can be synthesized in this same size range. The pIONPs are non-toxic and do not induce conventional pro-inflammatory cytokines in vitro and in vivo. We show that murine blood-stage antigen MSP4/5 from Plasmodium yoelii could be chemically conjugated to pIONPs and the use of these conjugates as immunogens led to the induction of both specific antibodies and IFNγ CD4+ T cells reactive to MSP4/5 in mice, comparable to responses to MSP4/5 mixed with classical adjuvants (e.g., CpG or Alum) that preferentially induce Th1 or Th2 cells individually. These results suggest that biodegradable pIONPs warrant further exploration as carriers for developing blood-stage malaria vaccines.
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spelling pubmed-77115412020-12-04 Pullulan-Coated Iron Oxide Nanoparticles for Blood-Stage Malaria Vaccine Delivery Powles, Liam Wilson, Kirsty L. Xiang, Sue D. Coppel, Ross L. Ma, Charles Selomulya, Cordelia Plebanski, Magdalena Vaccines (Basel) Article Vaccines against blood-stage malaria often aim to induce antibodies to neutralize parasite entry into red blood cells, interferon gamma (IFNγ) produced by T helper 1 (Th1) CD4+ T cells or interleukin 4 (IL-4) produced by T helper 2 (Th2) cells to provide B cell help. One vaccine delivery method for suitable putative malaria protein antigens is the use of nanoparticles as vaccine carriers. It has been previously shown that antigen conjugated to inorganic nanoparticles in the viral-particle size range (~40–60 nm) can induce protective antibodies and T cells against malaria antigens in a rodent malaria challenge model. Herein, it is shown that biodegradable pullulan-coated iron oxide nanoparticles (pIONPs) can be synthesized in this same size range. The pIONPs are non-toxic and do not induce conventional pro-inflammatory cytokines in vitro and in vivo. We show that murine blood-stage antigen MSP4/5 from Plasmodium yoelii could be chemically conjugated to pIONPs and the use of these conjugates as immunogens led to the induction of both specific antibodies and IFNγ CD4+ T cells reactive to MSP4/5 in mice, comparable to responses to MSP4/5 mixed with classical adjuvants (e.g., CpG or Alum) that preferentially induce Th1 or Th2 cells individually. These results suggest that biodegradable pIONPs warrant further exploration as carriers for developing blood-stage malaria vaccines. MDPI 2020-11-03 /pmc/articles/PMC7711541/ /pubmed/33153189 http://dx.doi.org/10.3390/vaccines8040651 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Powles, Liam
Wilson, Kirsty L.
Xiang, Sue D.
Coppel, Ross L.
Ma, Charles
Selomulya, Cordelia
Plebanski, Magdalena
Pullulan-Coated Iron Oxide Nanoparticles for Blood-Stage Malaria Vaccine Delivery
title Pullulan-Coated Iron Oxide Nanoparticles for Blood-Stage Malaria Vaccine Delivery
title_full Pullulan-Coated Iron Oxide Nanoparticles for Blood-Stage Malaria Vaccine Delivery
title_fullStr Pullulan-Coated Iron Oxide Nanoparticles for Blood-Stage Malaria Vaccine Delivery
title_full_unstemmed Pullulan-Coated Iron Oxide Nanoparticles for Blood-Stage Malaria Vaccine Delivery
title_short Pullulan-Coated Iron Oxide Nanoparticles for Blood-Stage Malaria Vaccine Delivery
title_sort pullulan-coated iron oxide nanoparticles for blood-stage malaria vaccine delivery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711541/
https://www.ncbi.nlm.nih.gov/pubmed/33153189
http://dx.doi.org/10.3390/vaccines8040651
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