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Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots

A portable reconfigurable platform for hemoglobin determination based on inner filter quenching of room-temperature phosphorescent carbon dots (CDs) in the presence of H(2)O(2) is described. The electronic setup consists of a light-emitting diode (LED) as the carbon dot optical exciter and a photodi...

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Autores principales: Murru, Fabio, Romero, Francisco J., Sánchez-Mudarra, Roberto, García Ruiz, Francisco J., Morales, Diego P., Capitán-Vallvey, Luis Fermín, Salinas-Castillo, Alfonso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711904/
https://www.ncbi.nlm.nih.gov/pubmed/32357422
http://dx.doi.org/10.3390/nano10050825
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author Murru, Fabio
Romero, Francisco J.
Sánchez-Mudarra, Roberto
García Ruiz, Francisco J.
Morales, Diego P.
Capitán-Vallvey, Luis Fermín
Salinas-Castillo, Alfonso
author_facet Murru, Fabio
Romero, Francisco J.
Sánchez-Mudarra, Roberto
García Ruiz, Francisco J.
Morales, Diego P.
Capitán-Vallvey, Luis Fermín
Salinas-Castillo, Alfonso
author_sort Murru, Fabio
collection PubMed
description A portable reconfigurable platform for hemoglobin determination based on inner filter quenching of room-temperature phosphorescent carbon dots (CDs) in the presence of H(2)O(2) is described. The electronic setup consists of a light-emitting diode (LED) as the carbon dot optical exciter and a photodiode as a light-to-current converter integrated in the same instrument. The reconfigurable feature provides adaptability to use the platform as an analytical probe for CDs coming from different batches with some variations in luminescence characteristics. The variables of the reaction were optimized, such as pH, concentration of reagents, and response time; as well as the variables of the portable device, such as LED voltage, photodiode sensitivity, and adjustment of the measuring range by a reconfigurable electronic system. The portable device allowed the determination of hemoglobin with good sensitivity, with a detection limit of 6.2 nM and range up to 125 nM.
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spelling pubmed-77119042020-12-04 Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots Murru, Fabio Romero, Francisco J. Sánchez-Mudarra, Roberto García Ruiz, Francisco J. Morales, Diego P. Capitán-Vallvey, Luis Fermín Salinas-Castillo, Alfonso Nanomaterials (Basel) Article A portable reconfigurable platform for hemoglobin determination based on inner filter quenching of room-temperature phosphorescent carbon dots (CDs) in the presence of H(2)O(2) is described. The electronic setup consists of a light-emitting diode (LED) as the carbon dot optical exciter and a photodiode as a light-to-current converter integrated in the same instrument. The reconfigurable feature provides adaptability to use the platform as an analytical probe for CDs coming from different batches with some variations in luminescence characteristics. The variables of the reaction were optimized, such as pH, concentration of reagents, and response time; as well as the variables of the portable device, such as LED voltage, photodiode sensitivity, and adjustment of the measuring range by a reconfigurable electronic system. The portable device allowed the determination of hemoglobin with good sensitivity, with a detection limit of 6.2 nM and range up to 125 nM. MDPI 2020-04-26 /pmc/articles/PMC7711904/ /pubmed/32357422 http://dx.doi.org/10.3390/nano10050825 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Murru, Fabio
Romero, Francisco J.
Sánchez-Mudarra, Roberto
García Ruiz, Francisco J.
Morales, Diego P.
Capitán-Vallvey, Luis Fermín
Salinas-Castillo, Alfonso
Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots
title Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots
title_full Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots
title_fullStr Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots
title_full_unstemmed Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots
title_short Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots
title_sort portable instrument for hemoglobin determination using room-temperature phosphorescent carbon dots
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711904/
https://www.ncbi.nlm.nih.gov/pubmed/32357422
http://dx.doi.org/10.3390/nano10050825
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