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Partial enzyme digestion facilitates regeneration of crushed nerve in rat
Peripheral nerve injury is a life-changing disability with significant socioeconomic consequences. In this rat model, we propose that partial enzyme digestion can facilitate the functional recovery of a crushed nerve. The sciatic nerves were harvested and in vitro cultured with the addition of Liber...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711954/ https://www.ncbi.nlm.nih.gov/pubmed/33335765 http://dx.doi.org/10.1515/tnsci-2020-0112 |
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author | Tsuang, Fon-Yih Chen, Ming-Hong Lin, Feng-Huei Yang, Ming-Chia Liao, Chun-Jen Chang, Wen-Hsiang Sun, Jui-Sheng |
author_facet | Tsuang, Fon-Yih Chen, Ming-Hong Lin, Feng-Huei Yang, Ming-Chia Liao, Chun-Jen Chang, Wen-Hsiang Sun, Jui-Sheng |
author_sort | Tsuang, Fon-Yih |
collection | PubMed |
description | Peripheral nerve injury is a life-changing disability with significant socioeconomic consequences. In this rat model, we propose that partial enzyme digestion can facilitate the functional recovery of a crushed nerve. The sciatic nerves were harvested and in vitro cultured with the addition of Liberase to determine the appropriate enzyme amount in the hyaluronic acid (HA) membrane. Then, the sciatic nerve of adult male Sprague-Dawley rats was exposed, crushed, and then treated with partial enzyme digestion (either 0.001 or 0.002 unit/mm(2) Liberase-HA membrane). The sciatic function index (SFI) for functional recovery of the sciatic nerve was evaluated. After 2 h of in vitro digestion, fascicles and axons were separated from each other, with the cells mobilized. Greater destruction of histology structures occurred in the high enzyme (Liberase-HA membrane at 0.002 unit/mm(2)) group at 24 h than in the low enzyme (0.001 unit/mm(2)) group at 48 h. In the SFI evaluation, the improvement in 0.001 unit/mm(2) Liberase group was significantly better than control and 0.002 unit/mm(2) Liberase group. Our study demonstrated that appropriate enzyme digestion had a significantly faster and earlier recovery. |
format | Online Article Text |
id | pubmed-7711954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-77119542020-12-16 Partial enzyme digestion facilitates regeneration of crushed nerve in rat Tsuang, Fon-Yih Chen, Ming-Hong Lin, Feng-Huei Yang, Ming-Chia Liao, Chun-Jen Chang, Wen-Hsiang Sun, Jui-Sheng Transl Neurosci Research Article Peripheral nerve injury is a life-changing disability with significant socioeconomic consequences. In this rat model, we propose that partial enzyme digestion can facilitate the functional recovery of a crushed nerve. The sciatic nerves were harvested and in vitro cultured with the addition of Liberase to determine the appropriate enzyme amount in the hyaluronic acid (HA) membrane. Then, the sciatic nerve of adult male Sprague-Dawley rats was exposed, crushed, and then treated with partial enzyme digestion (either 0.001 or 0.002 unit/mm(2) Liberase-HA membrane). The sciatic function index (SFI) for functional recovery of the sciatic nerve was evaluated. After 2 h of in vitro digestion, fascicles and axons were separated from each other, with the cells mobilized. Greater destruction of histology structures occurred in the high enzyme (Liberase-HA membrane at 0.002 unit/mm(2)) group at 24 h than in the low enzyme (0.001 unit/mm(2)) group at 48 h. In the SFI evaluation, the improvement in 0.001 unit/mm(2) Liberase group was significantly better than control and 0.002 unit/mm(2) Liberase group. Our study demonstrated that appropriate enzyme digestion had a significantly faster and earlier recovery. De Gruyter 2020-08-17 /pmc/articles/PMC7711954/ /pubmed/33335765 http://dx.doi.org/10.1515/tnsci-2020-0112 Text en © 2020 Fon-Yih Tsuang et al., published by De Gruyter http://creativecommons.org/licenses/by/4.0 This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Article Tsuang, Fon-Yih Chen, Ming-Hong Lin, Feng-Huei Yang, Ming-Chia Liao, Chun-Jen Chang, Wen-Hsiang Sun, Jui-Sheng Partial enzyme digestion facilitates regeneration of crushed nerve in rat |
title | Partial enzyme digestion facilitates regeneration of crushed nerve in rat |
title_full | Partial enzyme digestion facilitates regeneration of crushed nerve in rat |
title_fullStr | Partial enzyme digestion facilitates regeneration of crushed nerve in rat |
title_full_unstemmed | Partial enzyme digestion facilitates regeneration of crushed nerve in rat |
title_short | Partial enzyme digestion facilitates regeneration of crushed nerve in rat |
title_sort | partial enzyme digestion facilitates regeneration of crushed nerve in rat |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711954/ https://www.ncbi.nlm.nih.gov/pubmed/33335765 http://dx.doi.org/10.1515/tnsci-2020-0112 |
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