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Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil

Tandem repeat proteins (TRPs) are major immunoreactive proteins of Ehrlichia canis, which have been used in the serological diagnosis of different genotypes of the microorganism. TRP19 is preserved among different E. canis isolates expressed on both reticulate and dense-core cells and observed in th...

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Autores principales: Taques, Isis Indaiara Gonçalves Granjeiro, Campos, Amanda Noeli Silva, Kavasaki, Mayara Lima, de Almeida, Sayanne Luns Hatum, de Aguiar, Daniel Moura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712067/
https://www.ncbi.nlm.nih.gov/pubmed/33138252
http://dx.doi.org/10.3390/vetsci7040165
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author Taques, Isis Indaiara Gonçalves Granjeiro
Campos, Amanda Noeli Silva
Kavasaki, Mayara Lima
de Almeida, Sayanne Luns Hatum
de Aguiar, Daniel Moura
author_facet Taques, Isis Indaiara Gonçalves Granjeiro
Campos, Amanda Noeli Silva
Kavasaki, Mayara Lima
de Almeida, Sayanne Luns Hatum
de Aguiar, Daniel Moura
author_sort Taques, Isis Indaiara Gonçalves Granjeiro
collection PubMed
description Tandem repeat proteins (TRPs) are major immunoreactive proteins of Ehrlichia canis, which have been used in the serological diagnosis of different genotypes of the microorganism. TRP19 is preserved among different E. canis isolates expressed on both reticulate and dense-core cells and observed in the extracellular matrix or associated with the morula membrane. TRP36 is differentially expressed only on the surface of the dense-core form of the bacterium and exhibits more divergence among isolates. The aim of this study was to evaluate the distribution of the American (USTRP36), Brazilian (BrTRP36) and Costa Rican (CRTRP36) genotypes of E. canis in Brazil, using ELISA assays. Serum samples of 814 dogs from 49 municipalities from all over Brazil were analyzed. Our results showed that 34% of the samples were reactive to the USTRP36 genotype and 32.6% to the BrTRP36 genotype. The two genotypes appeared to occur equally throughout Brazil, although the frequency of seropositivity was lower in the south than in the country’s other regions. Dogs that reacted to at least one of the synthetic peptides (TRP19 and TRP36) were 456 (56%). A few dogs (n = 5; 0.61%) reactive to the E. canis TRP36 genotype (CRTRP36) were also detected in the northeast and southern regions. We concluded that the American and Brazilian genotypes of E. canis are distributed evenly in Brazil, especially in the tropical region, while the temperate region in the south presented the lowest prevalence rates. This study offers the first report of dogs seropositive for the Costa Rican genotype in Brazil.
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spelling pubmed-77120672020-12-04 Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil Taques, Isis Indaiara Gonçalves Granjeiro Campos, Amanda Noeli Silva Kavasaki, Mayara Lima de Almeida, Sayanne Luns Hatum de Aguiar, Daniel Moura Vet Sci Communication Tandem repeat proteins (TRPs) are major immunoreactive proteins of Ehrlichia canis, which have been used in the serological diagnosis of different genotypes of the microorganism. TRP19 is preserved among different E. canis isolates expressed on both reticulate and dense-core cells and observed in the extracellular matrix or associated with the morula membrane. TRP36 is differentially expressed only on the surface of the dense-core form of the bacterium and exhibits more divergence among isolates. The aim of this study was to evaluate the distribution of the American (USTRP36), Brazilian (BrTRP36) and Costa Rican (CRTRP36) genotypes of E. canis in Brazil, using ELISA assays. Serum samples of 814 dogs from 49 municipalities from all over Brazil were analyzed. Our results showed that 34% of the samples were reactive to the USTRP36 genotype and 32.6% to the BrTRP36 genotype. The two genotypes appeared to occur equally throughout Brazil, although the frequency of seropositivity was lower in the south than in the country’s other regions. Dogs that reacted to at least one of the synthetic peptides (TRP19 and TRP36) were 456 (56%). A few dogs (n = 5; 0.61%) reactive to the E. canis TRP36 genotype (CRTRP36) were also detected in the northeast and southern regions. We concluded that the American and Brazilian genotypes of E. canis are distributed evenly in Brazil, especially in the tropical region, while the temperate region in the south presented the lowest prevalence rates. This study offers the first report of dogs seropositive for the Costa Rican genotype in Brazil. MDPI 2020-10-29 /pmc/articles/PMC7712067/ /pubmed/33138252 http://dx.doi.org/10.3390/vetsci7040165 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Taques, Isis Indaiara Gonçalves Granjeiro
Campos, Amanda Noeli Silva
Kavasaki, Mayara Lima
de Almeida, Sayanne Luns Hatum
de Aguiar, Daniel Moura
Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil
title Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil
title_full Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil
title_fullStr Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil
title_full_unstemmed Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil
title_short Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil
title_sort geographic distribution of ehrlichia canis trp genotypes in brazil
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712067/
https://www.ncbi.nlm.nih.gov/pubmed/33138252
http://dx.doi.org/10.3390/vetsci7040165
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