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Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia

We evaluated the performance of the (1,3)-β-d-glucan (BDG) assay on bronchoalveolar lavage fluid (BALF) as a possible aid to the diagnosis of Pneumocystis jirovecii pneumonia. BALF samples from 18 patients with well-characterized proven, probable, and possible Pneumocystis pneumonia and 18 well-matc...

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Autores principales: Zhou, Shiwei, Linder, Kathleen A., Kauffman, Carol A., Richards, Blair J., Kleiboeker, Steve, Miceli, Marisa H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712134/
https://www.ncbi.nlm.nih.gov/pubmed/33019729
http://dx.doi.org/10.3390/jof6040200
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author Zhou, Shiwei
Linder, Kathleen A.
Kauffman, Carol A.
Richards, Blair J.
Kleiboeker, Steve
Miceli, Marisa H.
author_facet Zhou, Shiwei
Linder, Kathleen A.
Kauffman, Carol A.
Richards, Blair J.
Kleiboeker, Steve
Miceli, Marisa H.
author_sort Zhou, Shiwei
collection PubMed
description We evaluated the performance of the (1,3)-β-d-glucan (BDG) assay on bronchoalveolar lavage fluid (BALF) as a possible aid to the diagnosis of Pneumocystis jirovecii pneumonia. BALF samples from 18 patients with well-characterized proven, probable, and possible Pneumocystis pneumonia and 18 well-matched controls were tested. We found that the best test performance was observed with a cut-off value of 128 pg/mL; receiver operating characteristic/area under the curve (ROC/AUC) was 0.70 (95% CI 0.52–0.87). Sensitivity and specificity were 78% and 56%, respectively; positive predictive value was 64%, and negative predictive value was 71%. The low specificity that we noted limits the utility of BALF BDG as a diagnostic tool for Pneumocystis pneumonia.
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spelling pubmed-77121342020-12-04 Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia Zhou, Shiwei Linder, Kathleen A. Kauffman, Carol A. Richards, Blair J. Kleiboeker, Steve Miceli, Marisa H. J Fungi (Basel) Brief Report We evaluated the performance of the (1,3)-β-d-glucan (BDG) assay on bronchoalveolar lavage fluid (BALF) as a possible aid to the diagnosis of Pneumocystis jirovecii pneumonia. BALF samples from 18 patients with well-characterized proven, probable, and possible Pneumocystis pneumonia and 18 well-matched controls were tested. We found that the best test performance was observed with a cut-off value of 128 pg/mL; receiver operating characteristic/area under the curve (ROC/AUC) was 0.70 (95% CI 0.52–0.87). Sensitivity and specificity were 78% and 56%, respectively; positive predictive value was 64%, and negative predictive value was 71%. The low specificity that we noted limits the utility of BALF BDG as a diagnostic tool for Pneumocystis pneumonia. MDPI 2020-10-01 /pmc/articles/PMC7712134/ /pubmed/33019729 http://dx.doi.org/10.3390/jof6040200 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Zhou, Shiwei
Linder, Kathleen A.
Kauffman, Carol A.
Richards, Blair J.
Kleiboeker, Steve
Miceli, Marisa H.
Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia
title Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia
title_full Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia
title_fullStr Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia
title_full_unstemmed Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia
title_short Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia
title_sort diagnostic performance of bronchoalveolar lavage (1,3)-β-d-glucan assay for pneumocystis jirovecii pneumonia
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712134/
https://www.ncbi.nlm.nih.gov/pubmed/33019729
http://dx.doi.org/10.3390/jof6040200
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