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Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia
We evaluated the performance of the (1,3)-β-d-glucan (BDG) assay on bronchoalveolar lavage fluid (BALF) as a possible aid to the diagnosis of Pneumocystis jirovecii pneumonia. BALF samples from 18 patients with well-characterized proven, probable, and possible Pneumocystis pneumonia and 18 well-matc...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712134/ https://www.ncbi.nlm.nih.gov/pubmed/33019729 http://dx.doi.org/10.3390/jof6040200 |
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author | Zhou, Shiwei Linder, Kathleen A. Kauffman, Carol A. Richards, Blair J. Kleiboeker, Steve Miceli, Marisa H. |
author_facet | Zhou, Shiwei Linder, Kathleen A. Kauffman, Carol A. Richards, Blair J. Kleiboeker, Steve Miceli, Marisa H. |
author_sort | Zhou, Shiwei |
collection | PubMed |
description | We evaluated the performance of the (1,3)-β-d-glucan (BDG) assay on bronchoalveolar lavage fluid (BALF) as a possible aid to the diagnosis of Pneumocystis jirovecii pneumonia. BALF samples from 18 patients with well-characterized proven, probable, and possible Pneumocystis pneumonia and 18 well-matched controls were tested. We found that the best test performance was observed with a cut-off value of 128 pg/mL; receiver operating characteristic/area under the curve (ROC/AUC) was 0.70 (95% CI 0.52–0.87). Sensitivity and specificity were 78% and 56%, respectively; positive predictive value was 64%, and negative predictive value was 71%. The low specificity that we noted limits the utility of BALF BDG as a diagnostic tool for Pneumocystis pneumonia. |
format | Online Article Text |
id | pubmed-7712134 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77121342020-12-04 Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia Zhou, Shiwei Linder, Kathleen A. Kauffman, Carol A. Richards, Blair J. Kleiboeker, Steve Miceli, Marisa H. J Fungi (Basel) Brief Report We evaluated the performance of the (1,3)-β-d-glucan (BDG) assay on bronchoalveolar lavage fluid (BALF) as a possible aid to the diagnosis of Pneumocystis jirovecii pneumonia. BALF samples from 18 patients with well-characterized proven, probable, and possible Pneumocystis pneumonia and 18 well-matched controls were tested. We found that the best test performance was observed with a cut-off value of 128 pg/mL; receiver operating characteristic/area under the curve (ROC/AUC) was 0.70 (95% CI 0.52–0.87). Sensitivity and specificity were 78% and 56%, respectively; positive predictive value was 64%, and negative predictive value was 71%. The low specificity that we noted limits the utility of BALF BDG as a diagnostic tool for Pneumocystis pneumonia. MDPI 2020-10-01 /pmc/articles/PMC7712134/ /pubmed/33019729 http://dx.doi.org/10.3390/jof6040200 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Zhou, Shiwei Linder, Kathleen A. Kauffman, Carol A. Richards, Blair J. Kleiboeker, Steve Miceli, Marisa H. Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia |
title | Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia |
title_full | Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia |
title_fullStr | Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia |
title_full_unstemmed | Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia |
title_short | Diagnostic Performance of Bronchoalveolar Lavage (1,3)-β-d-Glucan Assay for Pneumocystis jirovecii Pneumonia |
title_sort | diagnostic performance of bronchoalveolar lavage (1,3)-β-d-glucan assay for pneumocystis jirovecii pneumonia |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712134/ https://www.ncbi.nlm.nih.gov/pubmed/33019729 http://dx.doi.org/10.3390/jof6040200 |
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