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Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with Paracoccidioides brasiliensis: More Is Not Necessarily Better
Most people infected with the fungus Paracoccidioides spp. do not get sick, but approximately 5% develop paracoccidioidomycosis. Understanding how host immunity determinants influence disease development could lead to novel preventative or therapeutic strategies; hence, we used two mouse strains tha...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712260/ https://www.ncbi.nlm.nih.gov/pubmed/33255176 http://dx.doi.org/10.3390/jof6040311 |
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author | de-Souza-Silva, Calliandra M. Hurtado, Fabián Andrés Tavares, Aldo Henrique de Oliveira, Getúlio P. Raiol, Taina Nishibe, Christiane Agustinho, Daniel Paiva Almeida, Nalvo Franco Walter, Maria Emília Machado Telles Nicola, André Moraes Bocca, Anamélia Lorenzetti Albuquerque, Patrícia Silva-Pereira, Ildinete |
author_facet | de-Souza-Silva, Calliandra M. Hurtado, Fabián Andrés Tavares, Aldo Henrique de Oliveira, Getúlio P. Raiol, Taina Nishibe, Christiane Agustinho, Daniel Paiva Almeida, Nalvo Franco Walter, Maria Emília Machado Telles Nicola, André Moraes Bocca, Anamélia Lorenzetti Albuquerque, Patrícia Silva-Pereira, Ildinete |
author_sort | de-Souza-Silva, Calliandra M. |
collection | PubMed |
description | Most people infected with the fungus Paracoccidioides spp. do not get sick, but approximately 5% develop paracoccidioidomycosis. Understanding how host immunity determinants influence disease development could lead to novel preventative or therapeutic strategies; hence, we used two mouse strains that are resistant (A/J) or susceptible (B10.A) to P. brasiliensis to study how dendritic cells (DCs) respond to the infection. RNA sequencing analysis showed that the susceptible strain DCs remodeled their transcriptomes much more intensely than those from the resistant strain, agreeing with a previous model of more intense innate immunity response in the susceptible strain. Contrastingly, these cells also repress genes/processes involved in antigen processing and presentation, such as lysosomal activity and autophagy. After the interaction with P. brasiliensis, both DCs and macrophages from the susceptible mouse reduced the autophagy marker LC3-II recruitment to the fungal phagosome compared to the resistant strain cells, confirming this pathway’s repression. These results suggest that impairment in antigen processing and presentation processes might be partially responsible for the inefficient activation of the adaptive immune response in this model. |
format | Online Article Text |
id | pubmed-7712260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77122602020-12-04 Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with Paracoccidioides brasiliensis: More Is Not Necessarily Better de-Souza-Silva, Calliandra M. Hurtado, Fabián Andrés Tavares, Aldo Henrique de Oliveira, Getúlio P. Raiol, Taina Nishibe, Christiane Agustinho, Daniel Paiva Almeida, Nalvo Franco Walter, Maria Emília Machado Telles Nicola, André Moraes Bocca, Anamélia Lorenzetti Albuquerque, Patrícia Silva-Pereira, Ildinete J Fungi (Basel) Article Most people infected with the fungus Paracoccidioides spp. do not get sick, but approximately 5% develop paracoccidioidomycosis. Understanding how host immunity determinants influence disease development could lead to novel preventative or therapeutic strategies; hence, we used two mouse strains that are resistant (A/J) or susceptible (B10.A) to P. brasiliensis to study how dendritic cells (DCs) respond to the infection. RNA sequencing analysis showed that the susceptible strain DCs remodeled their transcriptomes much more intensely than those from the resistant strain, agreeing with a previous model of more intense innate immunity response in the susceptible strain. Contrastingly, these cells also repress genes/processes involved in antigen processing and presentation, such as lysosomal activity and autophagy. After the interaction with P. brasiliensis, both DCs and macrophages from the susceptible mouse reduced the autophagy marker LC3-II recruitment to the fungal phagosome compared to the resistant strain cells, confirming this pathway’s repression. These results suggest that impairment in antigen processing and presentation processes might be partially responsible for the inefficient activation of the adaptive immune response in this model. MDPI 2020-11-24 /pmc/articles/PMC7712260/ /pubmed/33255176 http://dx.doi.org/10.3390/jof6040311 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de-Souza-Silva, Calliandra M. Hurtado, Fabián Andrés Tavares, Aldo Henrique de Oliveira, Getúlio P. Raiol, Taina Nishibe, Christiane Agustinho, Daniel Paiva Almeida, Nalvo Franco Walter, Maria Emília Machado Telles Nicola, André Moraes Bocca, Anamélia Lorenzetti Albuquerque, Patrícia Silva-Pereira, Ildinete Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with Paracoccidioides brasiliensis: More Is Not Necessarily Better |
title | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with Paracoccidioides brasiliensis: More Is Not Necessarily Better |
title_full | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with Paracoccidioides brasiliensis: More Is Not Necessarily Better |
title_fullStr | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with Paracoccidioides brasiliensis: More Is Not Necessarily Better |
title_full_unstemmed | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with Paracoccidioides brasiliensis: More Is Not Necessarily Better |
title_short | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with Paracoccidioides brasiliensis: More Is Not Necessarily Better |
title_sort | transcriptional remodeling patterns in murine dendritic cells infected with paracoccidioides brasiliensis: more is not necessarily better |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712260/ https://www.ncbi.nlm.nih.gov/pubmed/33255176 http://dx.doi.org/10.3390/jof6040311 |
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