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Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber
Parallel plate flow chambers are widely used to expose cultured cells to physiological flows for the investigation of a variety of diseases. These applications usually involve the generation of continuous and steady fluid flow over cell monolayers for extended durations, usually a few days. Another...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712398/ https://www.ncbi.nlm.nih.gov/pubmed/32987681 http://dx.doi.org/10.3390/mps3040065 |
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author | Shurbaji, Samar Al-Ruweidi, Mahmoud Khatib A. A. Ali, Fatma Hassan Benslimane, Fatiha M. Yalcin, Huseyin C. |
author_facet | Shurbaji, Samar Al-Ruweidi, Mahmoud Khatib A. A. Ali, Fatma Hassan Benslimane, Fatiha M. Yalcin, Huseyin C. |
author_sort | Shurbaji, Samar |
collection | PubMed |
description | Parallel plate flow chambers are widely used to expose cultured cells to physiological flows for the investigation of a variety of diseases. These applications usually involve the generation of continuous and steady fluid flow over cell monolayers for extended durations, usually a few days. Another technique is to generate a fast high-stress wave over the cells to see the immediate effect of flow-induced stresses. This can be achieved by propagating an air/liquid interface, in other words, a bubble, over cell monolayers. The approach is relevant to the reopening event of fluid-filled lung bronchioles and alveoli during mechanical ventilation therapy of Acute Respiratory Distress Syndrome. This article explains how we generate a stress wave using a parallel plate flow chamber and presents representative results of this wave on cultured lung epithelial cells. |
format | Online Article Text |
id | pubmed-7712398 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77123982020-12-04 Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber Shurbaji, Samar Al-Ruweidi, Mahmoud Khatib A. A. Ali, Fatma Hassan Benslimane, Fatiha M. Yalcin, Huseyin C. Methods Protoc Protocol Parallel plate flow chambers are widely used to expose cultured cells to physiological flows for the investigation of a variety of diseases. These applications usually involve the generation of continuous and steady fluid flow over cell monolayers for extended durations, usually a few days. Another technique is to generate a fast high-stress wave over the cells to see the immediate effect of flow-induced stresses. This can be achieved by propagating an air/liquid interface, in other words, a bubble, over cell monolayers. The approach is relevant to the reopening event of fluid-filled lung bronchioles and alveoli during mechanical ventilation therapy of Acute Respiratory Distress Syndrome. This article explains how we generate a stress wave using a parallel plate flow chamber and presents representative results of this wave on cultured lung epithelial cells. MDPI 2020-09-24 /pmc/articles/PMC7712398/ /pubmed/32987681 http://dx.doi.org/10.3390/mps3040065 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Shurbaji, Samar Al-Ruweidi, Mahmoud Khatib A. A. Ali, Fatma Hassan Benslimane, Fatiha M. Yalcin, Huseyin C. Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber |
title | Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber |
title_full | Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber |
title_fullStr | Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber |
title_full_unstemmed | Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber |
title_short | Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber |
title_sort | application of a flow-induced stress wave and investigation of associated injuries on cell monolayers using a parallel plate flow chamber |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712398/ https://www.ncbi.nlm.nih.gov/pubmed/32987681 http://dx.doi.org/10.3390/mps3040065 |
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