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Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China

Though several Diaporthe species have been reported in China, little is known about the species associated with nature reserves in Guizhou province. During a survey of fungi in six nature reserves in Guizhou province of China, thirty-one Diaporthe isolates were collected from different woody hosts....

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Autores principales: Dissanayake, Asha J., Chen, Ya-Ya, Liu, Jian-Kui (Jack)
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712415/
https://www.ncbi.nlm.nih.gov/pubmed/33121032
http://dx.doi.org/10.3390/jof6040251
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author Dissanayake, Asha J.
Chen, Ya-Ya
Liu, Jian-Kui (Jack)
author_facet Dissanayake, Asha J.
Chen, Ya-Ya
Liu, Jian-Kui (Jack)
author_sort Dissanayake, Asha J.
collection PubMed
description Though several Diaporthe species have been reported in China, little is known about the species associated with nature reserves in Guizhou province. During a survey of fungi in six nature reserves in Guizhou province of China, thirty-one Diaporthe isolates were collected from different woody hosts. Based on morphology, culture characteristics and molecular phylogenetic analysis, these isolates were characterized and identified. Phylogenetic analysis of internal transcribed spacer region (ITS), combined with translation elongation factor 1-alpha (tef), β-tubulin (tub), calmodulin (cal) and histone H3 (his) gene regions identified five known Diaporthe species and seven distinct lineages representing novel Diaporthe species. The details of five known species: Diaporthe cercidis, D. cinnamomi, D. conica, D. nobilis and D. sackstonii are given and the seven new species D. constrictospora, D. ellipsospora, D. guttulata, D. irregularis, D. lenispora, D. minima, and D. minusculata are introduced with detailed descriptions and illustrations. This study revealed a high diversity of previously undescribed Diaporthe species associated with woody hosts in various nature reserves of Guizhou province, indicating that there is a potential of Diaporthe species remains to be discovered in this unique landform (Karst formations) in China. Interestingly, the five known Diaporthe species have been reported as pathogens of various hosts, and this could indicate that those newly introduced species in this study could be potentially pathogenic pending further studies to confirm.
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spelling pubmed-77124152020-12-04 Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China Dissanayake, Asha J. Chen, Ya-Ya Liu, Jian-Kui (Jack) J Fungi (Basel) Article Though several Diaporthe species have been reported in China, little is known about the species associated with nature reserves in Guizhou province. During a survey of fungi in six nature reserves in Guizhou province of China, thirty-one Diaporthe isolates were collected from different woody hosts. Based on morphology, culture characteristics and molecular phylogenetic analysis, these isolates were characterized and identified. Phylogenetic analysis of internal transcribed spacer region (ITS), combined with translation elongation factor 1-alpha (tef), β-tubulin (tub), calmodulin (cal) and histone H3 (his) gene regions identified five known Diaporthe species and seven distinct lineages representing novel Diaporthe species. The details of five known species: Diaporthe cercidis, D. cinnamomi, D. conica, D. nobilis and D. sackstonii are given and the seven new species D. constrictospora, D. ellipsospora, D. guttulata, D. irregularis, D. lenispora, D. minima, and D. minusculata are introduced with detailed descriptions and illustrations. This study revealed a high diversity of previously undescribed Diaporthe species associated with woody hosts in various nature reserves of Guizhou province, indicating that there is a potential of Diaporthe species remains to be discovered in this unique landform (Karst formations) in China. Interestingly, the five known Diaporthe species have been reported as pathogens of various hosts, and this could indicate that those newly introduced species in this study could be potentially pathogenic pending further studies to confirm. MDPI 2020-10-27 /pmc/articles/PMC7712415/ /pubmed/33121032 http://dx.doi.org/10.3390/jof6040251 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dissanayake, Asha J.
Chen, Ya-Ya
Liu, Jian-Kui (Jack)
Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China
title Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China
title_full Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China
title_fullStr Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China
title_full_unstemmed Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China
title_short Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China
title_sort unravelling diaporthe species associated with woody hosts from karst formations (guizhou) in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712415/
https://www.ncbi.nlm.nih.gov/pubmed/33121032
http://dx.doi.org/10.3390/jof6040251
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