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Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma

Onychomycosis is one of the most common nail disorders. Its current treatment is not satisfactorily effective and often causes adverse side effects. This study aims to determine the optimal conditions for non-thermal plasma (NTP) inactivation of the most common dermatophytes in vitro and to apply it...

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Autores principales: Lux, Jaroslav, Dobiáš, Radim, Kuklová, Ivana, Litvik, Radek, Scholtz, Vladimír, Soušková, Hana, Khun, Josef, Mrázek, Jakub, Kantorová, Michaela, Jaworská, Pavla, Prejdová, Táňa, Šnupárková, Jana, Hamal, Petr, Julák, Jaroslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712512/
https://www.ncbi.nlm.nih.gov/pubmed/33050542
http://dx.doi.org/10.3390/jof6040214
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author Lux, Jaroslav
Dobiáš, Radim
Kuklová, Ivana
Litvik, Radek
Scholtz, Vladimír
Soušková, Hana
Khun, Josef
Mrázek, Jakub
Kantorová, Michaela
Jaworská, Pavla
Prejdová, Táňa
Šnupárková, Jana
Hamal, Petr
Julák, Jaroslav
author_facet Lux, Jaroslav
Dobiáš, Radim
Kuklová, Ivana
Litvik, Radek
Scholtz, Vladimír
Soušková, Hana
Khun, Josef
Mrázek, Jakub
Kantorová, Michaela
Jaworská, Pavla
Prejdová, Táňa
Šnupárková, Jana
Hamal, Petr
Julák, Jaroslav
author_sort Lux, Jaroslav
collection PubMed
description Onychomycosis is one of the most common nail disorders. Its current treatment is not satisfactorily effective and often causes adverse side effects. This study aims to determine the optimal conditions for non-thermal plasma (NTP) inactivation of the most common dermatophytes in vitro and to apply it in patient`s therapy. The in vitro exposure to NTP produced by negative DC corona discharge caused full inactivation of Trichophyton spp. if applied during the early growth phases. This effect decreased to negligible inactivation with the exposure applied six days after inoculation. In a group of 40 patients with onychomycosis, NTP therapy was combined with nail plate abrasion and refreshment (NPAR) or treatment with antimycotics. The cohort included 17 patients treated with NPAR combined with NTP, 11 patients treated with antimycotics and NTP, and 12 patients treated with NPAR alone. The combination of NPAR and NTP resulted in clinical cure in more than 70% of patients. The synergistic effect of NPAR and NTP caused 85.7% improvement of mycological cure confirmed by negative microscopy and culture of the affected nail plate. We conclude that NTP can significantly improve the treatment of onychomycosis.
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spelling pubmed-77125122020-12-04 Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma Lux, Jaroslav Dobiáš, Radim Kuklová, Ivana Litvik, Radek Scholtz, Vladimír Soušková, Hana Khun, Josef Mrázek, Jakub Kantorová, Michaela Jaworská, Pavla Prejdová, Táňa Šnupárková, Jana Hamal, Petr Julák, Jaroslav J Fungi (Basel) Article Onychomycosis is one of the most common nail disorders. Its current treatment is not satisfactorily effective and often causes adverse side effects. This study aims to determine the optimal conditions for non-thermal plasma (NTP) inactivation of the most common dermatophytes in vitro and to apply it in patient`s therapy. The in vitro exposure to NTP produced by negative DC corona discharge caused full inactivation of Trichophyton spp. if applied during the early growth phases. This effect decreased to negligible inactivation with the exposure applied six days after inoculation. In a group of 40 patients with onychomycosis, NTP therapy was combined with nail plate abrasion and refreshment (NPAR) or treatment with antimycotics. The cohort included 17 patients treated with NPAR combined with NTP, 11 patients treated with antimycotics and NTP, and 12 patients treated with NPAR alone. The combination of NPAR and NTP resulted in clinical cure in more than 70% of patients. The synergistic effect of NPAR and NTP caused 85.7% improvement of mycological cure confirmed by negative microscopy and culture of the affected nail plate. We conclude that NTP can significantly improve the treatment of onychomycosis. MDPI 2020-10-10 /pmc/articles/PMC7712512/ /pubmed/33050542 http://dx.doi.org/10.3390/jof6040214 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lux, Jaroslav
Dobiáš, Radim
Kuklová, Ivana
Litvik, Radek
Scholtz, Vladimír
Soušková, Hana
Khun, Josef
Mrázek, Jakub
Kantorová, Michaela
Jaworská, Pavla
Prejdová, Táňa
Šnupárková, Jana
Hamal, Petr
Julák, Jaroslav
Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma
title Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma
title_full Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma
title_fullStr Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma
title_full_unstemmed Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma
title_short Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma
title_sort inactivation of dermatophytes causing onychomycosis and its therapy using non-thermal plasma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712512/
https://www.ncbi.nlm.nih.gov/pubmed/33050542
http://dx.doi.org/10.3390/jof6040214
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