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Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering

BACKGROUND: The physical factors of the extracellular matrix have a profound influence on the differentiation behavior of mesenchymal stem cells. In this study, the effect of the biophysical microenvironment on rat bone marrow mesenchymal stem cell (BMSC) osteogenesis was studied both in vitro and i...

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Autores principales: Yang, Yuchao, Feng, Yanting, Qu, Rongmei, Li, Qingtao, Rong, Dongming, Fan, Tingyu, Yang, Yiting, Sun, Bing, Bi, Zhenyu, Khan, Asmat Ullah, Deng, Ting, Dai, Jingxing, Ouyang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712560/
https://www.ncbi.nlm.nih.gov/pubmed/33272329
http://dx.doi.org/10.1186/s13287-020-02024-8
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author Yang, Yuchao
Feng, Yanting
Qu, Rongmei
Li, Qingtao
Rong, Dongming
Fan, Tingyu
Yang, Yiting
Sun, Bing
Bi, Zhenyu
Khan, Asmat Ullah
Deng, Ting
Dai, Jingxing
Ouyang, Jun
author_facet Yang, Yuchao
Feng, Yanting
Qu, Rongmei
Li, Qingtao
Rong, Dongming
Fan, Tingyu
Yang, Yiting
Sun, Bing
Bi, Zhenyu
Khan, Asmat Ullah
Deng, Ting
Dai, Jingxing
Ouyang, Jun
author_sort Yang, Yuchao
collection PubMed
description BACKGROUND: The physical factors of the extracellular matrix have a profound influence on the differentiation behavior of mesenchymal stem cells. In this study, the effect of the biophysical microenvironment on rat bone marrow mesenchymal stem cell (BMSC) osteogenesis was studied both in vitro and in vivo. METHODS: To prepare cell culture scaffolds of varying stiffness, increasing amounts of hydroxyapatite (HAp) were mixed into a polyethylene glycol/silk fibroin (PEG/SF) solution. The amount of HAp ranged from 25 to 100 mg, which provided for different ratios between HAp and the PEG/SF composite. In vitro, the effect of stiffness on the osteogenic differentiation of rat BMSCs was studied. The outcome measures, which were verified in vivo, included the protein expression of runt-related transcription factor 2 and osteocalcin, alkaline phosphatase activity, and the mRNA expression of osteogenesis-related markers. RESULTS: Increasing amounts of HAp resulted in an increased elastic modulus of the cell culture scaffolds. The PEG/SF/HAp fabricated with HAp (50 mg) significantly increased cell adhesion and viability (p < 0.05) as well as the expression of all the osteogenesis-related markers (p < 0.05). CONCLUSIONS: We developed a novel cell culture scaffold and demonstrated that substrate stiffness influenced the osteogenic differentiation of rat BMSCs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-020-02024-8.
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spelling pubmed-77125602020-12-03 Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering Yang, Yuchao Feng, Yanting Qu, Rongmei Li, Qingtao Rong, Dongming Fan, Tingyu Yang, Yiting Sun, Bing Bi, Zhenyu Khan, Asmat Ullah Deng, Ting Dai, Jingxing Ouyang, Jun Stem Cell Res Ther Research BACKGROUND: The physical factors of the extracellular matrix have a profound influence on the differentiation behavior of mesenchymal stem cells. In this study, the effect of the biophysical microenvironment on rat bone marrow mesenchymal stem cell (BMSC) osteogenesis was studied both in vitro and in vivo. METHODS: To prepare cell culture scaffolds of varying stiffness, increasing amounts of hydroxyapatite (HAp) were mixed into a polyethylene glycol/silk fibroin (PEG/SF) solution. The amount of HAp ranged from 25 to 100 mg, which provided for different ratios between HAp and the PEG/SF composite. In vitro, the effect of stiffness on the osteogenic differentiation of rat BMSCs was studied. The outcome measures, which were verified in vivo, included the protein expression of runt-related transcription factor 2 and osteocalcin, alkaline phosphatase activity, and the mRNA expression of osteogenesis-related markers. RESULTS: Increasing amounts of HAp resulted in an increased elastic modulus of the cell culture scaffolds. The PEG/SF/HAp fabricated with HAp (50 mg) significantly increased cell adhesion and viability (p < 0.05) as well as the expression of all the osteogenesis-related markers (p < 0.05). CONCLUSIONS: We developed a novel cell culture scaffold and demonstrated that substrate stiffness influenced the osteogenic differentiation of rat BMSCs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-020-02024-8. BioMed Central 2020-12-03 /pmc/articles/PMC7712560/ /pubmed/33272329 http://dx.doi.org/10.1186/s13287-020-02024-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Yang, Yuchao
Feng, Yanting
Qu, Rongmei
Li, Qingtao
Rong, Dongming
Fan, Tingyu
Yang, Yiting
Sun, Bing
Bi, Zhenyu
Khan, Asmat Ullah
Deng, Ting
Dai, Jingxing
Ouyang, Jun
Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering
title Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering
title_full Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering
title_fullStr Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering
title_full_unstemmed Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering
title_short Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering
title_sort synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712560/
https://www.ncbi.nlm.nih.gov/pubmed/33272329
http://dx.doi.org/10.1186/s13287-020-02024-8
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