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Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line

Bovine endometritis is a serious pathogen-induced infectious disease that affects the physiological processes of estrus, pregnancy and the postpartum condition. The inflamed endometrium responds by activating an inflammatory intracellular signaling cascade that leads to increased expression of proin...

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Autores principales: Gugliandolo, Enrico, Fusco, Roberta, Licata, Patrizia, Peritore, Alessio Filippo, D’amico, Ramona, Cordaro, Marika, Siracusa, Rosalba, Cuzzocrea, Salvatore, Crupi, Rosalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712648/
https://www.ncbi.nlm.nih.gov/pubmed/33114084
http://dx.doi.org/10.3390/vetsci7040161
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author Gugliandolo, Enrico
Fusco, Roberta
Licata, Patrizia
Peritore, Alessio Filippo
D’amico, Ramona
Cordaro, Marika
Siracusa, Rosalba
Cuzzocrea, Salvatore
Crupi, Rosalia
author_facet Gugliandolo, Enrico
Fusco, Roberta
Licata, Patrizia
Peritore, Alessio Filippo
D’amico, Ramona
Cordaro, Marika
Siracusa, Rosalba
Cuzzocrea, Salvatore
Crupi, Rosalia
author_sort Gugliandolo, Enrico
collection PubMed
description Bovine endometritis is a serious pathogen-induced infectious disease that affects the physiological processes of estrus, pregnancy and the postpartum condition. The inflamed endometrium responds by activating an inflammatory intracellular signaling cascade that leads to increased expression of proinflammatory cytokines and reactive oxygen species (ROS). Oxidative stress is closely related to several pathological conditions in perinatal dairy cows and play a key role in tissue damage. Hydroxytyrosol (HT), a natural phenolic alcohol with a strong antioxidant activity, displayed a wide range of biological effect. The aim of this study was to evaluate the protective effects of HT in an in vitro model of lipopolysaccharide (LPS)-induced inflammation in bovine uterine endometrial cells. Our results showed that HT had a significant protective effect in LPS-induced inflammation and oxidative stress. HT was also able to increase the capacity of endogenous antioxidant systems through the up-regulation of the NRF2 pathway. Furthermore, HT restored the tight junction protein expressions. In conclusion, our results showed the protective effects of HT in LPS-stimulated BEND cells. Therefore, the results of this study suggest an important protective role of HT in the treatment and prevention of uterine pathologies in dairy cows.
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spelling pubmed-77126482020-12-04 Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line Gugliandolo, Enrico Fusco, Roberta Licata, Patrizia Peritore, Alessio Filippo D’amico, Ramona Cordaro, Marika Siracusa, Rosalba Cuzzocrea, Salvatore Crupi, Rosalia Vet Sci Article Bovine endometritis is a serious pathogen-induced infectious disease that affects the physiological processes of estrus, pregnancy and the postpartum condition. The inflamed endometrium responds by activating an inflammatory intracellular signaling cascade that leads to increased expression of proinflammatory cytokines and reactive oxygen species (ROS). Oxidative stress is closely related to several pathological conditions in perinatal dairy cows and play a key role in tissue damage. Hydroxytyrosol (HT), a natural phenolic alcohol with a strong antioxidant activity, displayed a wide range of biological effect. The aim of this study was to evaluate the protective effects of HT in an in vitro model of lipopolysaccharide (LPS)-induced inflammation in bovine uterine endometrial cells. Our results showed that HT had a significant protective effect in LPS-induced inflammation and oxidative stress. HT was also able to increase the capacity of endogenous antioxidant systems through the up-regulation of the NRF2 pathway. Furthermore, HT restored the tight junction protein expressions. In conclusion, our results showed the protective effects of HT in LPS-stimulated BEND cells. Therefore, the results of this study suggest an important protective role of HT in the treatment and prevention of uterine pathologies in dairy cows. MDPI 2020-10-23 /pmc/articles/PMC7712648/ /pubmed/33114084 http://dx.doi.org/10.3390/vetsci7040161 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gugliandolo, Enrico
Fusco, Roberta
Licata, Patrizia
Peritore, Alessio Filippo
D’amico, Ramona
Cordaro, Marika
Siracusa, Rosalba
Cuzzocrea, Salvatore
Crupi, Rosalia
Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line
title Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line
title_full Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line
title_fullStr Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line
title_full_unstemmed Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line
title_short Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line
title_sort protective effect of hydroxytyrosol on lps-induced inflammation and oxidative stress in bovine endometrial epithelial cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712648/
https://www.ncbi.nlm.nih.gov/pubmed/33114084
http://dx.doi.org/10.3390/vetsci7040161
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