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Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line
Bovine endometritis is a serious pathogen-induced infectious disease that affects the physiological processes of estrus, pregnancy and the postpartum condition. The inflamed endometrium responds by activating an inflammatory intracellular signaling cascade that leads to increased expression of proin...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712648/ https://www.ncbi.nlm.nih.gov/pubmed/33114084 http://dx.doi.org/10.3390/vetsci7040161 |
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author | Gugliandolo, Enrico Fusco, Roberta Licata, Patrizia Peritore, Alessio Filippo D’amico, Ramona Cordaro, Marika Siracusa, Rosalba Cuzzocrea, Salvatore Crupi, Rosalia |
author_facet | Gugliandolo, Enrico Fusco, Roberta Licata, Patrizia Peritore, Alessio Filippo D’amico, Ramona Cordaro, Marika Siracusa, Rosalba Cuzzocrea, Salvatore Crupi, Rosalia |
author_sort | Gugliandolo, Enrico |
collection | PubMed |
description | Bovine endometritis is a serious pathogen-induced infectious disease that affects the physiological processes of estrus, pregnancy and the postpartum condition. The inflamed endometrium responds by activating an inflammatory intracellular signaling cascade that leads to increased expression of proinflammatory cytokines and reactive oxygen species (ROS). Oxidative stress is closely related to several pathological conditions in perinatal dairy cows and play a key role in tissue damage. Hydroxytyrosol (HT), a natural phenolic alcohol with a strong antioxidant activity, displayed a wide range of biological effect. The aim of this study was to evaluate the protective effects of HT in an in vitro model of lipopolysaccharide (LPS)-induced inflammation in bovine uterine endometrial cells. Our results showed that HT had a significant protective effect in LPS-induced inflammation and oxidative stress. HT was also able to increase the capacity of endogenous antioxidant systems through the up-regulation of the NRF2 pathway. Furthermore, HT restored the tight junction protein expressions. In conclusion, our results showed the protective effects of HT in LPS-stimulated BEND cells. Therefore, the results of this study suggest an important protective role of HT in the treatment and prevention of uterine pathologies in dairy cows. |
format | Online Article Text |
id | pubmed-7712648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77126482020-12-04 Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line Gugliandolo, Enrico Fusco, Roberta Licata, Patrizia Peritore, Alessio Filippo D’amico, Ramona Cordaro, Marika Siracusa, Rosalba Cuzzocrea, Salvatore Crupi, Rosalia Vet Sci Article Bovine endometritis is a serious pathogen-induced infectious disease that affects the physiological processes of estrus, pregnancy and the postpartum condition. The inflamed endometrium responds by activating an inflammatory intracellular signaling cascade that leads to increased expression of proinflammatory cytokines and reactive oxygen species (ROS). Oxidative stress is closely related to several pathological conditions in perinatal dairy cows and play a key role in tissue damage. Hydroxytyrosol (HT), a natural phenolic alcohol with a strong antioxidant activity, displayed a wide range of biological effect. The aim of this study was to evaluate the protective effects of HT in an in vitro model of lipopolysaccharide (LPS)-induced inflammation in bovine uterine endometrial cells. Our results showed that HT had a significant protective effect in LPS-induced inflammation and oxidative stress. HT was also able to increase the capacity of endogenous antioxidant systems through the up-regulation of the NRF2 pathway. Furthermore, HT restored the tight junction protein expressions. In conclusion, our results showed the protective effects of HT in LPS-stimulated BEND cells. Therefore, the results of this study suggest an important protective role of HT in the treatment and prevention of uterine pathologies in dairy cows. MDPI 2020-10-23 /pmc/articles/PMC7712648/ /pubmed/33114084 http://dx.doi.org/10.3390/vetsci7040161 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gugliandolo, Enrico Fusco, Roberta Licata, Patrizia Peritore, Alessio Filippo D’amico, Ramona Cordaro, Marika Siracusa, Rosalba Cuzzocrea, Salvatore Crupi, Rosalia Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line |
title | Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line |
title_full | Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line |
title_fullStr | Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line |
title_full_unstemmed | Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line |
title_short | Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line |
title_sort | protective effect of hydroxytyrosol on lps-induced inflammation and oxidative stress in bovine endometrial epithelial cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712648/ https://www.ncbi.nlm.nih.gov/pubmed/33114084 http://dx.doi.org/10.3390/vetsci7040161 |
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