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Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1

Since 2003, H5N1 highly pathogenic avian influenza viruses (HPAIV) have not only caused outbreaks in poultry but were also transmitted to humans with high mortality rates. Vaccination is an efficient and economical means of increasing immunity against infections to decrease the shedding of infectiou...

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Autores principales: Phan, Hoang Trong, Pham, Van Thi, Ho, Thuong Thi, Pham, Ngoc Bich, Chu, Ha Hoang, Vu, Trang Huyen, Abdelwhab, Elsayed M., Scheibner, David, Mettenleiter, Thomas C., Hanh, Tran Xuan, Meister, Armin, Gresch, Ulrike, Conrad, Udo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712794/
https://www.ncbi.nlm.nih.gov/pubmed/33050224
http://dx.doi.org/10.3390/vaccines8040593
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author Phan, Hoang Trong
Pham, Van Thi
Ho, Thuong Thi
Pham, Ngoc Bich
Chu, Ha Hoang
Vu, Trang Huyen
Abdelwhab, Elsayed M.
Scheibner, David
Mettenleiter, Thomas C.
Hanh, Tran Xuan
Meister, Armin
Gresch, Ulrike
Conrad, Udo
author_facet Phan, Hoang Trong
Pham, Van Thi
Ho, Thuong Thi
Pham, Ngoc Bich
Chu, Ha Hoang
Vu, Trang Huyen
Abdelwhab, Elsayed M.
Scheibner, David
Mettenleiter, Thomas C.
Hanh, Tran Xuan
Meister, Armin
Gresch, Ulrike
Conrad, Udo
author_sort Phan, Hoang Trong
collection PubMed
description Since 2003, H5N1 highly pathogenic avian influenza viruses (HPAIV) have not only caused outbreaks in poultry but were also transmitted to humans with high mortality rates. Vaccination is an efficient and economical means of increasing immunity against infections to decrease the shedding of infectious agents in immunized animals and to reduce the probability of further infections. Subunit vaccines from plants are the focus of modern vaccine developments. In this study, plant-made hemagglutinin (H5) trimers were purified from transiently transformed N. benthamiana plants. All chickens immunized with purified H5 trimers were fully protected against the severe HPAIV H5N1 challenge. We further developed a proof-of-principle approach by using disulfide bonds, homoantiparallel peptides or homodimer proteins to combine H5 trimers leading to production of H5 oligomers. Mice vaccinated with crude leaf extracts containing H5 oligomers induced neutralizing antibodies better than those induced by crude leaf extracts containing trimers. As a major result, eleven out of twelve chickens (92%) immunized with adjuvanted H5 oligomer crude extracts were protected from lethal disease while nine out of twelve chickens (75%) vaccinated with adjuvanted H5 trimer crude extracts survived. The solid protective immune response achieved by immunization with crude extracts and the stability of the oligomers form the basis for the development of inexpensive protective veterinary vaccines.
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spelling pubmed-77127942020-12-04 Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1 Phan, Hoang Trong Pham, Van Thi Ho, Thuong Thi Pham, Ngoc Bich Chu, Ha Hoang Vu, Trang Huyen Abdelwhab, Elsayed M. Scheibner, David Mettenleiter, Thomas C. Hanh, Tran Xuan Meister, Armin Gresch, Ulrike Conrad, Udo Vaccines (Basel) Article Since 2003, H5N1 highly pathogenic avian influenza viruses (HPAIV) have not only caused outbreaks in poultry but were also transmitted to humans with high mortality rates. Vaccination is an efficient and economical means of increasing immunity against infections to decrease the shedding of infectious agents in immunized animals and to reduce the probability of further infections. Subunit vaccines from plants are the focus of modern vaccine developments. In this study, plant-made hemagglutinin (H5) trimers were purified from transiently transformed N. benthamiana plants. All chickens immunized with purified H5 trimers were fully protected against the severe HPAIV H5N1 challenge. We further developed a proof-of-principle approach by using disulfide bonds, homoantiparallel peptides or homodimer proteins to combine H5 trimers leading to production of H5 oligomers. Mice vaccinated with crude leaf extracts containing H5 oligomers induced neutralizing antibodies better than those induced by crude leaf extracts containing trimers. As a major result, eleven out of twelve chickens (92%) immunized with adjuvanted H5 oligomer crude extracts were protected from lethal disease while nine out of twelve chickens (75%) vaccinated with adjuvanted H5 trimer crude extracts survived. The solid protective immune response achieved by immunization with crude extracts and the stability of the oligomers form the basis for the development of inexpensive protective veterinary vaccines. MDPI 2020-10-09 /pmc/articles/PMC7712794/ /pubmed/33050224 http://dx.doi.org/10.3390/vaccines8040593 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Phan, Hoang Trong
Pham, Van Thi
Ho, Thuong Thi
Pham, Ngoc Bich
Chu, Ha Hoang
Vu, Trang Huyen
Abdelwhab, Elsayed M.
Scheibner, David
Mettenleiter, Thomas C.
Hanh, Tran Xuan
Meister, Armin
Gresch, Ulrike
Conrad, Udo
Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1
title Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1
title_full Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1
title_fullStr Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1
title_full_unstemmed Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1
title_short Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1
title_sort immunization with plant-derived multimeric h5 hemagglutinins protect chicken against highly pathogenic avian influenza virus h5n1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712794/
https://www.ncbi.nlm.nih.gov/pubmed/33050224
http://dx.doi.org/10.3390/vaccines8040593
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