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Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1
Since 2003, H5N1 highly pathogenic avian influenza viruses (HPAIV) have not only caused outbreaks in poultry but were also transmitted to humans with high mortality rates. Vaccination is an efficient and economical means of increasing immunity against infections to decrease the shedding of infectiou...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712794/ https://www.ncbi.nlm.nih.gov/pubmed/33050224 http://dx.doi.org/10.3390/vaccines8040593 |
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author | Phan, Hoang Trong Pham, Van Thi Ho, Thuong Thi Pham, Ngoc Bich Chu, Ha Hoang Vu, Trang Huyen Abdelwhab, Elsayed M. Scheibner, David Mettenleiter, Thomas C. Hanh, Tran Xuan Meister, Armin Gresch, Ulrike Conrad, Udo |
author_facet | Phan, Hoang Trong Pham, Van Thi Ho, Thuong Thi Pham, Ngoc Bich Chu, Ha Hoang Vu, Trang Huyen Abdelwhab, Elsayed M. Scheibner, David Mettenleiter, Thomas C. Hanh, Tran Xuan Meister, Armin Gresch, Ulrike Conrad, Udo |
author_sort | Phan, Hoang Trong |
collection | PubMed |
description | Since 2003, H5N1 highly pathogenic avian influenza viruses (HPAIV) have not only caused outbreaks in poultry but were also transmitted to humans with high mortality rates. Vaccination is an efficient and economical means of increasing immunity against infections to decrease the shedding of infectious agents in immunized animals and to reduce the probability of further infections. Subunit vaccines from plants are the focus of modern vaccine developments. In this study, plant-made hemagglutinin (H5) trimers were purified from transiently transformed N. benthamiana plants. All chickens immunized with purified H5 trimers were fully protected against the severe HPAIV H5N1 challenge. We further developed a proof-of-principle approach by using disulfide bonds, homoantiparallel peptides or homodimer proteins to combine H5 trimers leading to production of H5 oligomers. Mice vaccinated with crude leaf extracts containing H5 oligomers induced neutralizing antibodies better than those induced by crude leaf extracts containing trimers. As a major result, eleven out of twelve chickens (92%) immunized with adjuvanted H5 oligomer crude extracts were protected from lethal disease while nine out of twelve chickens (75%) vaccinated with adjuvanted H5 trimer crude extracts survived. The solid protective immune response achieved by immunization with crude extracts and the stability of the oligomers form the basis for the development of inexpensive protective veterinary vaccines. |
format | Online Article Text |
id | pubmed-7712794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77127942020-12-04 Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1 Phan, Hoang Trong Pham, Van Thi Ho, Thuong Thi Pham, Ngoc Bich Chu, Ha Hoang Vu, Trang Huyen Abdelwhab, Elsayed M. Scheibner, David Mettenleiter, Thomas C. Hanh, Tran Xuan Meister, Armin Gresch, Ulrike Conrad, Udo Vaccines (Basel) Article Since 2003, H5N1 highly pathogenic avian influenza viruses (HPAIV) have not only caused outbreaks in poultry but were also transmitted to humans with high mortality rates. Vaccination is an efficient and economical means of increasing immunity against infections to decrease the shedding of infectious agents in immunized animals and to reduce the probability of further infections. Subunit vaccines from plants are the focus of modern vaccine developments. In this study, plant-made hemagglutinin (H5) trimers were purified from transiently transformed N. benthamiana plants. All chickens immunized with purified H5 trimers were fully protected against the severe HPAIV H5N1 challenge. We further developed a proof-of-principle approach by using disulfide bonds, homoantiparallel peptides or homodimer proteins to combine H5 trimers leading to production of H5 oligomers. Mice vaccinated with crude leaf extracts containing H5 oligomers induced neutralizing antibodies better than those induced by crude leaf extracts containing trimers. As a major result, eleven out of twelve chickens (92%) immunized with adjuvanted H5 oligomer crude extracts were protected from lethal disease while nine out of twelve chickens (75%) vaccinated with adjuvanted H5 trimer crude extracts survived. The solid protective immune response achieved by immunization with crude extracts and the stability of the oligomers form the basis for the development of inexpensive protective veterinary vaccines. MDPI 2020-10-09 /pmc/articles/PMC7712794/ /pubmed/33050224 http://dx.doi.org/10.3390/vaccines8040593 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Phan, Hoang Trong Pham, Van Thi Ho, Thuong Thi Pham, Ngoc Bich Chu, Ha Hoang Vu, Trang Huyen Abdelwhab, Elsayed M. Scheibner, David Mettenleiter, Thomas C. Hanh, Tran Xuan Meister, Armin Gresch, Ulrike Conrad, Udo Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1 |
title | Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1 |
title_full | Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1 |
title_fullStr | Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1 |
title_full_unstemmed | Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1 |
title_short | Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1 |
title_sort | immunization with plant-derived multimeric h5 hemagglutinins protect chicken against highly pathogenic avian influenza virus h5n1 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712794/ https://www.ncbi.nlm.nih.gov/pubmed/33050224 http://dx.doi.org/10.3390/vaccines8040593 |
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