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The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments

Progress in metabolic engineering and synthetic and systems biology has made bioproduction an increasingly attractive and competitive strategy for synthesizing biomolecules, recombinant proteins and biofuels from renewable feedstocks. Yet, due to poor productivity, it remains difficult to make a bio...

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Autores principales: Pouzet, Sylvain, Banderas, Alvaro, Le Bec, Matthias, Lautier, Thomas, Truan, Gilles, Hersen, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712799/
https://www.ncbi.nlm.nih.gov/pubmed/33255280
http://dx.doi.org/10.3390/bioengineering7040151
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author Pouzet, Sylvain
Banderas, Alvaro
Le Bec, Matthias
Lautier, Thomas
Truan, Gilles
Hersen, Pascal
author_facet Pouzet, Sylvain
Banderas, Alvaro
Le Bec, Matthias
Lautier, Thomas
Truan, Gilles
Hersen, Pascal
author_sort Pouzet, Sylvain
collection PubMed
description Progress in metabolic engineering and synthetic and systems biology has made bioproduction an increasingly attractive and competitive strategy for synthesizing biomolecules, recombinant proteins and biofuels from renewable feedstocks. Yet, due to poor productivity, it remains difficult to make a bioproduction process economically viable at large scale. Achieving dynamic control of cellular processes could lead to even better yields by balancing the two characteristic phases of bioproduction, namely, growth versus production, which lie at the heart of a trade-off that substantially impacts productivity. The versatility and controllability offered by light will be a key element in attaining the level of control desired. The popularity of light-mediated control is increasing, with an expanding repertoire of optogenetic systems for novel applications, and many optogenetic devices have been designed to test optogenetic strains at various culture scales for bioproduction objectives. In this review, we aim to highlight the most important advances in this direction. We discuss how optogenetics is currently applied to control metabolism in the context of bioproduction, describe the optogenetic instruments and devices used at the laboratory scale for strain development, and explore how current industrial-scale bioproduction processes could be adapted for optogenetics or could benefit from existing photobioreactor designs. We then draw attention to the steps that must be undertaken to further optimize the control of biological systems in order to take full advantage of the potential offered by microbial factories.
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spelling pubmed-77127992020-12-04 The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments Pouzet, Sylvain Banderas, Alvaro Le Bec, Matthias Lautier, Thomas Truan, Gilles Hersen, Pascal Bioengineering (Basel) Review Progress in metabolic engineering and synthetic and systems biology has made bioproduction an increasingly attractive and competitive strategy for synthesizing biomolecules, recombinant proteins and biofuels from renewable feedstocks. Yet, due to poor productivity, it remains difficult to make a bioproduction process economically viable at large scale. Achieving dynamic control of cellular processes could lead to even better yields by balancing the two characteristic phases of bioproduction, namely, growth versus production, which lie at the heart of a trade-off that substantially impacts productivity. The versatility and controllability offered by light will be a key element in attaining the level of control desired. The popularity of light-mediated control is increasing, with an expanding repertoire of optogenetic systems for novel applications, and many optogenetic devices have been designed to test optogenetic strains at various culture scales for bioproduction objectives. In this review, we aim to highlight the most important advances in this direction. We discuss how optogenetics is currently applied to control metabolism in the context of bioproduction, describe the optogenetic instruments and devices used at the laboratory scale for strain development, and explore how current industrial-scale bioproduction processes could be adapted for optogenetics or could benefit from existing photobioreactor designs. We then draw attention to the steps that must be undertaken to further optimize the control of biological systems in order to take full advantage of the potential offered by microbial factories. MDPI 2020-11-24 /pmc/articles/PMC7712799/ /pubmed/33255280 http://dx.doi.org/10.3390/bioengineering7040151 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pouzet, Sylvain
Banderas, Alvaro
Le Bec, Matthias
Lautier, Thomas
Truan, Gilles
Hersen, Pascal
The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments
title The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments
title_full The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments
title_fullStr The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments
title_full_unstemmed The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments
title_short The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments
title_sort promise of optogenetics for bioproduction: dynamic control strategies and scale-up instruments
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712799/
https://www.ncbi.nlm.nih.gov/pubmed/33255280
http://dx.doi.org/10.3390/bioengineering7040151
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