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The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments
Progress in metabolic engineering and synthetic and systems biology has made bioproduction an increasingly attractive and competitive strategy for synthesizing biomolecules, recombinant proteins and biofuels from renewable feedstocks. Yet, due to poor productivity, it remains difficult to make a bio...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712799/ https://www.ncbi.nlm.nih.gov/pubmed/33255280 http://dx.doi.org/10.3390/bioengineering7040151 |
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author | Pouzet, Sylvain Banderas, Alvaro Le Bec, Matthias Lautier, Thomas Truan, Gilles Hersen, Pascal |
author_facet | Pouzet, Sylvain Banderas, Alvaro Le Bec, Matthias Lautier, Thomas Truan, Gilles Hersen, Pascal |
author_sort | Pouzet, Sylvain |
collection | PubMed |
description | Progress in metabolic engineering and synthetic and systems biology has made bioproduction an increasingly attractive and competitive strategy for synthesizing biomolecules, recombinant proteins and biofuels from renewable feedstocks. Yet, due to poor productivity, it remains difficult to make a bioproduction process economically viable at large scale. Achieving dynamic control of cellular processes could lead to even better yields by balancing the two characteristic phases of bioproduction, namely, growth versus production, which lie at the heart of a trade-off that substantially impacts productivity. The versatility and controllability offered by light will be a key element in attaining the level of control desired. The popularity of light-mediated control is increasing, with an expanding repertoire of optogenetic systems for novel applications, and many optogenetic devices have been designed to test optogenetic strains at various culture scales for bioproduction objectives. In this review, we aim to highlight the most important advances in this direction. We discuss how optogenetics is currently applied to control metabolism in the context of bioproduction, describe the optogenetic instruments and devices used at the laboratory scale for strain development, and explore how current industrial-scale bioproduction processes could be adapted for optogenetics or could benefit from existing photobioreactor designs. We then draw attention to the steps that must be undertaken to further optimize the control of biological systems in order to take full advantage of the potential offered by microbial factories. |
format | Online Article Text |
id | pubmed-7712799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77127992020-12-04 The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments Pouzet, Sylvain Banderas, Alvaro Le Bec, Matthias Lautier, Thomas Truan, Gilles Hersen, Pascal Bioengineering (Basel) Review Progress in metabolic engineering and synthetic and systems biology has made bioproduction an increasingly attractive and competitive strategy for synthesizing biomolecules, recombinant proteins and biofuels from renewable feedstocks. Yet, due to poor productivity, it remains difficult to make a bioproduction process economically viable at large scale. Achieving dynamic control of cellular processes could lead to even better yields by balancing the two characteristic phases of bioproduction, namely, growth versus production, which lie at the heart of a trade-off that substantially impacts productivity. The versatility and controllability offered by light will be a key element in attaining the level of control desired. The popularity of light-mediated control is increasing, with an expanding repertoire of optogenetic systems for novel applications, and many optogenetic devices have been designed to test optogenetic strains at various culture scales for bioproduction objectives. In this review, we aim to highlight the most important advances in this direction. We discuss how optogenetics is currently applied to control metabolism in the context of bioproduction, describe the optogenetic instruments and devices used at the laboratory scale for strain development, and explore how current industrial-scale bioproduction processes could be adapted for optogenetics or could benefit from existing photobioreactor designs. We then draw attention to the steps that must be undertaken to further optimize the control of biological systems in order to take full advantage of the potential offered by microbial factories. MDPI 2020-11-24 /pmc/articles/PMC7712799/ /pubmed/33255280 http://dx.doi.org/10.3390/bioengineering7040151 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Pouzet, Sylvain Banderas, Alvaro Le Bec, Matthias Lautier, Thomas Truan, Gilles Hersen, Pascal The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments |
title | The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments |
title_full | The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments |
title_fullStr | The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments |
title_full_unstemmed | The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments |
title_short | The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments |
title_sort | promise of optogenetics for bioproduction: dynamic control strategies and scale-up instruments |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712799/ https://www.ncbi.nlm.nih.gov/pubmed/33255280 http://dx.doi.org/10.3390/bioengineering7040151 |
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