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Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes

Lentinula edodes is a globally important edible mushroom species that is appreciated for its medicinal properties as well as its nutritional value. During commercial cultivation, a mycelial brown film forms on the surface of the sawdust growth medium at the late vegetative stage. Mycelial film forma...

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Autores principales: Hong, Chang Pyo, Moon, Suyun, Yoo, Seung-il, Noh, Jong-Hyun, Ko, Han-Gyu, Kim, Hyun A., Ro, Hyeon-Su, Cho, Hyunwoo, Chung, Jong-Wook, Lee, Hwa-Yong, Ryu, Hojin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712820/
https://www.ncbi.nlm.nih.gov/pubmed/33182449
http://dx.doi.org/10.3390/jof6040272
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author Hong, Chang Pyo
Moon, Suyun
Yoo, Seung-il
Noh, Jong-Hyun
Ko, Han-Gyu
Kim, Hyun A.
Ro, Hyeon-Su
Cho, Hyunwoo
Chung, Jong-Wook
Lee, Hwa-Yong
Ryu, Hojin
author_facet Hong, Chang Pyo
Moon, Suyun
Yoo, Seung-il
Noh, Jong-Hyun
Ko, Han-Gyu
Kim, Hyun A.
Ro, Hyeon-Su
Cho, Hyunwoo
Chung, Jong-Wook
Lee, Hwa-Yong
Ryu, Hojin
author_sort Hong, Chang Pyo
collection PubMed
description Lentinula edodes is a globally important edible mushroom species that is appreciated for its medicinal properties as well as its nutritional value. During commercial cultivation, a mycelial brown film forms on the surface of the sawdust growth medium at the late vegetative stage. Mycelial film formation is a critical developmental process that contributes to the quantity and quality of the mushroom yield. However, little is known regarding the genetic underpinnings of brown film formation on the surface of mycelial tissue. A novel causal gene associated with the formation of the mycelial brown film, named ABL (Abnormal browning related to light), was identified in this study. The comparative genetic analysis by dihybrid crosses between normal and abnormal browning film cultivars demonstrated that a single dominant allele was responsible for the abnormal mycelium browning phenotype. Whole-genome sequencing analysis of hybrid isolates revealed five missense single-nucleotide polymorphisms (SNPs) in the ABL locus of individuals forming abnormal partial brown films. Additional whole-genome resequencing of a further 16 cultivars showed that three of the five missense SNPs were strongly associated with the abnormal browning phenotype. Overexpression of the dominant abl-D allele in a wild-type background conferred the abnormal mycelial browning phenotype upon transformants, with slender hyphae observed as a general defective mycelial growth phenotype. Our methodology will aid the future discovery of candidate genes associated with favorable traits in edible mushrooms. The discovery of a novel gene, ABL, associated with mycelial film formation will facilitate marker-associated breeding in L. edodes.
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spelling pubmed-77128202020-12-04 Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes Hong, Chang Pyo Moon, Suyun Yoo, Seung-il Noh, Jong-Hyun Ko, Han-Gyu Kim, Hyun A. Ro, Hyeon-Su Cho, Hyunwoo Chung, Jong-Wook Lee, Hwa-Yong Ryu, Hojin J Fungi (Basel) Article Lentinula edodes is a globally important edible mushroom species that is appreciated for its medicinal properties as well as its nutritional value. During commercial cultivation, a mycelial brown film forms on the surface of the sawdust growth medium at the late vegetative stage. Mycelial film formation is a critical developmental process that contributes to the quantity and quality of the mushroom yield. However, little is known regarding the genetic underpinnings of brown film formation on the surface of mycelial tissue. A novel causal gene associated with the formation of the mycelial brown film, named ABL (Abnormal browning related to light), was identified in this study. The comparative genetic analysis by dihybrid crosses between normal and abnormal browning film cultivars demonstrated that a single dominant allele was responsible for the abnormal mycelium browning phenotype. Whole-genome sequencing analysis of hybrid isolates revealed five missense single-nucleotide polymorphisms (SNPs) in the ABL locus of individuals forming abnormal partial brown films. Additional whole-genome resequencing of a further 16 cultivars showed that three of the five missense SNPs were strongly associated with the abnormal browning phenotype. Overexpression of the dominant abl-D allele in a wild-type background conferred the abnormal mycelial browning phenotype upon transformants, with slender hyphae observed as a general defective mycelial growth phenotype. Our methodology will aid the future discovery of candidate genes associated with favorable traits in edible mushrooms. The discovery of a novel gene, ABL, associated with mycelial film formation will facilitate marker-associated breeding in L. edodes. MDPI 2020-11-09 /pmc/articles/PMC7712820/ /pubmed/33182449 http://dx.doi.org/10.3390/jof6040272 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hong, Chang Pyo
Moon, Suyun
Yoo, Seung-il
Noh, Jong-Hyun
Ko, Han-Gyu
Kim, Hyun A.
Ro, Hyeon-Su
Cho, Hyunwoo
Chung, Jong-Wook
Lee, Hwa-Yong
Ryu, Hojin
Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes
title Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes
title_full Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes
title_fullStr Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes
title_full_unstemmed Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes
title_short Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes
title_sort functional analysis of a novel abl (abnormal browning related to light) gene in mycelial brown film formation of lentinula edodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712820/
https://www.ncbi.nlm.nih.gov/pubmed/33182449
http://dx.doi.org/10.3390/jof6040272
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