Cargando…
Description of Kinetic Behavior of Pathogenic Escherichia coli in Cooked Pig Trotters under Dynamic Storage Conditions Using Mathematical Equations
A dynamic model was developed to predict the Escherichia coli cell counts in pig trotters at changing temperatures. Five-strain mixture of pathogenic E. coli at 4 Log CFU/g were inoculated to cooked pig trotter samples. The samples were stored at 10°C, 20°C, and 25°C. The cell count data was analyze...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Food Science of Animal Resources
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713768/ https://www.ncbi.nlm.nih.gov/pubmed/33305278 http://dx.doi.org/10.5851/kosfa.2020.e64 |
_version_ | 1783618613690761216 |
---|---|
author | Ha, Jimyeong Lee, Jeeyeon Oh, Hyemin Kim, Hyun Jung Choi, Yukyung Lee, Yewon Kim, Yujin Lee, Heeyoung Kim, Sejeong Yoon, Yohan |
author_facet | Ha, Jimyeong Lee, Jeeyeon Oh, Hyemin Kim, Hyun Jung Choi, Yukyung Lee, Yewon Kim, Yujin Lee, Heeyoung Kim, Sejeong Yoon, Yohan |
author_sort | Ha, Jimyeong |
collection | PubMed |
description | A dynamic model was developed to predict the Escherichia coli cell counts in pig trotters at changing temperatures. Five-strain mixture of pathogenic E. coli at 4 Log CFU/g were inoculated to cooked pig trotter samples. The samples were stored at 10°C, 20°C, and 25°C. The cell count data was analyzed with the Baranyi model to compute the maximum specific growth rate (μ(max)) (Log CFU/g/h) and lag phase duration (LPD) (h). The kinetic parameters were analyzed using a polynomial equation, and a dynamic model was developed using the kinetic models. The model performance was evaluated using the accuracy factor (A(f)), bias factor (B(f)), and root mean square error (RMSE). E. coli cell counts increased (p<0.05) in pig trotter samples at all storage temperatures (10°C–25°C). LPD decreased (p<0.05) and μ(max) increased (p<0.05) as storage temperature increased. In addition, the value of h(0) was similar at 10°C and 20°C, implying that the physiological state was similar between 10°C and 20°C. The secondary models used were appropriate to evaluate the effect of storage temperature on LPD and μ(max). The developed kinetic models showed good performance with RMSE of 0.618, B(f) of 1.02, and A(f) of 1.08. Also, performance of the dynamic model was appropriate. Thus, the developed dynamic model in this study can be applied to describe the kinetic behavior of E. coli in cooked pig trotters during storage. |
format | Online Article Text |
id | pubmed-7713768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society for Food Science of Animal Resources |
record_format | MEDLINE/PubMed |
spelling | pubmed-77137682020-12-09 Description of Kinetic Behavior of Pathogenic Escherichia coli in Cooked Pig Trotters under Dynamic Storage Conditions Using Mathematical Equations Ha, Jimyeong Lee, Jeeyeon Oh, Hyemin Kim, Hyun Jung Choi, Yukyung Lee, Yewon Kim, Yujin Lee, Heeyoung Kim, Sejeong Yoon, Yohan Food Sci Anim Resour Article A dynamic model was developed to predict the Escherichia coli cell counts in pig trotters at changing temperatures. Five-strain mixture of pathogenic E. coli at 4 Log CFU/g were inoculated to cooked pig trotter samples. The samples were stored at 10°C, 20°C, and 25°C. The cell count data was analyzed with the Baranyi model to compute the maximum specific growth rate (μ(max)) (Log CFU/g/h) and lag phase duration (LPD) (h). The kinetic parameters were analyzed using a polynomial equation, and a dynamic model was developed using the kinetic models. The model performance was evaluated using the accuracy factor (A(f)), bias factor (B(f)), and root mean square error (RMSE). E. coli cell counts increased (p<0.05) in pig trotter samples at all storage temperatures (10°C–25°C). LPD decreased (p<0.05) and μ(max) increased (p<0.05) as storage temperature increased. In addition, the value of h(0) was similar at 10°C and 20°C, implying that the physiological state was similar between 10°C and 20°C. The secondary models used were appropriate to evaluate the effect of storage temperature on LPD and μ(max). The developed kinetic models showed good performance with RMSE of 0.618, B(f) of 1.02, and A(f) of 1.08. Also, performance of the dynamic model was appropriate. Thus, the developed dynamic model in this study can be applied to describe the kinetic behavior of E. coli in cooked pig trotters during storage. Korean Society for Food Science of Animal Resources 2020-11 2020-11-01 /pmc/articles/PMC7713768/ /pubmed/33305278 http://dx.doi.org/10.5851/kosfa.2020.e64 Text en © Korean Society for Food Science of Animal Resources http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Ha, Jimyeong Lee, Jeeyeon Oh, Hyemin Kim, Hyun Jung Choi, Yukyung Lee, Yewon Kim, Yujin Lee, Heeyoung Kim, Sejeong Yoon, Yohan Description of Kinetic Behavior of Pathogenic Escherichia coli in Cooked Pig Trotters under Dynamic Storage Conditions Using Mathematical Equations |
title | Description of Kinetic Behavior of Pathogenic Escherichia
coli in Cooked Pig Trotters under Dynamic Storage Conditions Using
Mathematical Equations |
title_full | Description of Kinetic Behavior of Pathogenic Escherichia
coli in Cooked Pig Trotters under Dynamic Storage Conditions Using
Mathematical Equations |
title_fullStr | Description of Kinetic Behavior of Pathogenic Escherichia
coli in Cooked Pig Trotters under Dynamic Storage Conditions Using
Mathematical Equations |
title_full_unstemmed | Description of Kinetic Behavior of Pathogenic Escherichia
coli in Cooked Pig Trotters under Dynamic Storage Conditions Using
Mathematical Equations |
title_short | Description of Kinetic Behavior of Pathogenic Escherichia
coli in Cooked Pig Trotters under Dynamic Storage Conditions Using
Mathematical Equations |
title_sort | description of kinetic behavior of pathogenic escherichia
coli in cooked pig trotters under dynamic storage conditions using
mathematical equations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713768/ https://www.ncbi.nlm.nih.gov/pubmed/33305278 http://dx.doi.org/10.5851/kosfa.2020.e64 |
work_keys_str_mv | AT hajimyeong descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations AT leejeeyeon descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations AT ohhyemin descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations AT kimhyunjung descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations AT choiyukyung descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations AT leeyewon descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations AT kimyujin descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations AT leeheeyoung descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations AT kimsejeong descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations AT yoonyohan descriptionofkineticbehaviorofpathogenicescherichiacoliincookedpigtrottersunderdynamicstorageconditionsusingmathematicalequations |