Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir.

To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) Wes...

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Detalles Bibliográficos
Autores principales: Johnson, Benziwa Nathalie, Quashie, Marie Luce Akossiwoa, Chaix, Gilles, Camus‐Kulandaivelu, Letizia, Adjonou, Kossi, Segla, Kossi Novinyo, Kokutse, Adzo Dzifa, Ouinsavi, Christine, Bationo, Babou André, Rabiou, Habou, Kokou, Kouami, Vignes, Hélène
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713909/
https://www.ncbi.nlm.nih.gov/pubmed/33304547
http://dx.doi.org/10.1002/ece3.6944
Descripción
Sumario:To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it.

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