Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir.

To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) Wes...

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Autores principales: Johnson, Benziwa Nathalie, Quashie, Marie Luce Akossiwoa, Chaix, Gilles, Camus‐Kulandaivelu, Letizia, Adjonou, Kossi, Segla, Kossi Novinyo, Kokutse, Adzo Dzifa, Ouinsavi, Christine, Bationo, Babou André, Rabiou, Habou, Kokou, Kouami, Vignes, Hélène
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713909/
https://www.ncbi.nlm.nih.gov/pubmed/33304547
http://dx.doi.org/10.1002/ece3.6944
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author Johnson, Benziwa Nathalie
Quashie, Marie Luce Akossiwoa
Chaix, Gilles
Camus‐Kulandaivelu, Letizia
Adjonou, Kossi
Segla, Kossi Novinyo
Kokutse, Adzo Dzifa
Ouinsavi, Christine
Bationo, Babou André
Rabiou, Habou
Kokou, Kouami
Vignes, Hélène
author_facet Johnson, Benziwa Nathalie
Quashie, Marie Luce Akossiwoa
Chaix, Gilles
Camus‐Kulandaivelu, Letizia
Adjonou, Kossi
Segla, Kossi Novinyo
Kokutse, Adzo Dzifa
Ouinsavi, Christine
Bationo, Babou André
Rabiou, Habou
Kokou, Kouami
Vignes, Hélène
author_sort Johnson, Benziwa Nathalie
collection PubMed
description To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it.
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spelling pubmed-77139092020-12-09 Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir. Johnson, Benziwa Nathalie Quashie, Marie Luce Akossiwoa Chaix, Gilles Camus‐Kulandaivelu, Letizia Adjonou, Kossi Segla, Kossi Novinyo Kokutse, Adzo Dzifa Ouinsavi, Christine Bationo, Babou André Rabiou, Habou Kokou, Kouami Vignes, Hélène Ecol Evol Original Research To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it. John Wiley and Sons Inc. 2020-10-27 /pmc/articles/PMC7713909/ /pubmed/33304547 http://dx.doi.org/10.1002/ece3.6944 Text en © 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Johnson, Benziwa Nathalie
Quashie, Marie Luce Akossiwoa
Chaix, Gilles
Camus‐Kulandaivelu, Letizia
Adjonou, Kossi
Segla, Kossi Novinyo
Kokutse, Adzo Dzifa
Ouinsavi, Christine
Bationo, Babou André
Rabiou, Habou
Kokou, Kouami
Vignes, Hélène
Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir.
title Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir.
title_full Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir.
title_fullStr Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir.
title_full_unstemmed Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir.
title_short Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir.
title_sort isolation and characterization of microsatellite markers for the threatened african endemic tree species pterocarpus erinaceus poir.
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713909/
https://www.ncbi.nlm.nih.gov/pubmed/33304547
http://dx.doi.org/10.1002/ece3.6944
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