Cargando…
Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water
1. The early detection of invasive non‐native species (INNS) is important for informing management actions. Established monitoring methods require the collection or observation of specimens, which is unlikely at the beginning of an invasion when densities are likely to be low. Environmental DNA (eDN...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713958/ https://www.ncbi.nlm.nih.gov/pubmed/33304534 http://dx.doi.org/10.1002/ece3.6921 |
_version_ | 1783618652496461824 |
---|---|
author | Blackman, Rosetta C. Ling, Kar Keun Sean Harper, Lynsey R. Shum, Peter Hänfling, Bernd Lawson‐Handley, Lori |
author_facet | Blackman, Rosetta C. Ling, Kar Keun Sean Harper, Lynsey R. Shum, Peter Hänfling, Bernd Lawson‐Handley, Lori |
author_sort | Blackman, Rosetta C. |
collection | PubMed |
description | 1. The early detection of invasive non‐native species (INNS) is important for informing management actions. Established monitoring methods require the collection or observation of specimens, which is unlikely at the beginning of an invasion when densities are likely to be low. Environmental DNA (eDNA) analysis is a highly promising technique for the detection of INNS—particularly during the early stages of an invasion. 2. Here, we compared the use of traditional kick‐net sampling with two eDNA approaches (targeted detection using both conventional and quantitative PCR and passive detection via metabarcoding with conserved primers) for detection of quagga mussel, Dreissena rostriformis bugensis, a high priority INNS, along a density gradient on the River Wraysbury, UK. 3. All three molecular tools outperformed traditional sampling in terms of detection. Conventional PCR and qPCR both had 100% detection rate in all samples and outperformed metabarcoding when the target species was at low densities. Additionally, quagga mussel DNA copy number (qPCR) and relative read count (metabarcoding) were significantly influenced by both mussel density and distance from source population, with distance being the most significant predictor. 4. Synthesis and application. All three molecular approaches were more sensitive than traditional kick‐net sampling for the detection of the quagga mussel in flowing water, and both qPCR and metabarcoding enabled estimates of relative abundance. Targeted approaches were more sensitive than metabarcoding, but metabarcoding has the advantage of providing information on the wider community and consequently the impacts of INNS. |
format | Online Article Text |
id | pubmed-7713958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77139582020-12-09 Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water Blackman, Rosetta C. Ling, Kar Keun Sean Harper, Lynsey R. Shum, Peter Hänfling, Bernd Lawson‐Handley, Lori Ecol Evol Original Research 1. The early detection of invasive non‐native species (INNS) is important for informing management actions. Established monitoring methods require the collection or observation of specimens, which is unlikely at the beginning of an invasion when densities are likely to be low. Environmental DNA (eDNA) analysis is a highly promising technique for the detection of INNS—particularly during the early stages of an invasion. 2. Here, we compared the use of traditional kick‐net sampling with two eDNA approaches (targeted detection using both conventional and quantitative PCR and passive detection via metabarcoding with conserved primers) for detection of quagga mussel, Dreissena rostriformis bugensis, a high priority INNS, along a density gradient on the River Wraysbury, UK. 3. All three molecular tools outperformed traditional sampling in terms of detection. Conventional PCR and qPCR both had 100% detection rate in all samples and outperformed metabarcoding when the target species was at low densities. Additionally, quagga mussel DNA copy number (qPCR) and relative read count (metabarcoding) were significantly influenced by both mussel density and distance from source population, with distance being the most significant predictor. 4. Synthesis and application. All three molecular approaches were more sensitive than traditional kick‐net sampling for the detection of the quagga mussel in flowing water, and both qPCR and metabarcoding enabled estimates of relative abundance. Targeted approaches were more sensitive than metabarcoding, but metabarcoding has the advantage of providing information on the wider community and consequently the impacts of INNS. John Wiley and Sons Inc. 2020-10-29 /pmc/articles/PMC7713958/ /pubmed/33304534 http://dx.doi.org/10.1002/ece3.6921 Text en © 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Blackman, Rosetta C. Ling, Kar Keun Sean Harper, Lynsey R. Shum, Peter Hänfling, Bernd Lawson‐Handley, Lori Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water |
title | Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water |
title_full | Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water |
title_fullStr | Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water |
title_full_unstemmed | Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water |
title_short | Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water |
title_sort | targeted and passive environmental dna approaches outperform established methods for detection of quagga mussels, dreissena rostriformis bugensis in flowing water |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713958/ https://www.ncbi.nlm.nih.gov/pubmed/33304534 http://dx.doi.org/10.1002/ece3.6921 |
work_keys_str_mv | AT blackmanrosettac targetedandpassiveenvironmentaldnaapproachesoutperformestablishedmethodsfordetectionofquaggamusselsdreissenarostriformisbugensisinflowingwater AT lingkarkeunsean targetedandpassiveenvironmentaldnaapproachesoutperformestablishedmethodsfordetectionofquaggamusselsdreissenarostriformisbugensisinflowingwater AT harperlynseyr targetedandpassiveenvironmentaldnaapproachesoutperformestablishedmethodsfordetectionofquaggamusselsdreissenarostriformisbugensisinflowingwater AT shumpeter targetedandpassiveenvironmentaldnaapproachesoutperformestablishedmethodsfordetectionofquaggamusselsdreissenarostriformisbugensisinflowingwater AT hanflingbernd targetedandpassiveenvironmentaldnaapproachesoutperformestablishedmethodsfordetectionofquaggamusselsdreissenarostriformisbugensisinflowingwater AT lawsonhandleylori targetedandpassiveenvironmentaldnaapproachesoutperformestablishedmethodsfordetectionofquaggamusselsdreissenarostriformisbugensisinflowingwater |